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Article: Oral colonization of aerobic and facultatively anaerobic gram-negative rods and cocci in irradiated, dentate, xerostomic individuals

TitleOral colonization of aerobic and facultatively anaerobic gram-negative rods and cocci in irradiated, dentate, xerostomic individuals
Authors
Issue Date2001
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/OMI
Citation
Oral Microbiology and Immunology, 2001, v. 16 n. 1, p. 1-9 How to Cite?
AbstractThis study aimed at investigating the oral colonization of aerobic and facultatively anaerobic gram-negative rods and cocci in head- and neck-irradiated, dentate, xerostomic individuals. Subjects were recruited from a nasopharyngeal carcinoma clinic and were segregated into group A: <60 years (n=25, 48±6years, 5±5 years post-irradiation) and group B: ≥60 years (n=8, 67±4 years, 2±2 years post-irradiation) and were compared with age- and sex-matched normal individuals, group C: <60 years (n=20, 44±12 years) and group D: ≥60 years (n=10, 70±3 years). Selective culture of the oral rinse samples was carried out to isolate, quantify and speciate (using API 20E kit) aerobic and facultatively anaerobic gram-negative rods and cocci recovery. All test subjects were put under comprehensive oral and preventive care for 3 months, and 12 group A and 5 group B subjects were recalled for reassessment of aerobic and facultatively anaerobic gram-negative rods and cocci colonization. All identical isolates, pre- and post-hygienic care, were phenotypically (Vitek, Hazelwood, MA and antibiogram profile) and genotypically (pulsed-field gel electrophoresis) evaluated. The aerobic and facultatively anaerobic gram-negative rods and cocci isolated from the first round oral rinse samples included: Acinetobacter, Neisseria, Chryseomonas, Flavimonas, Pseudomonas, Citrobacter, Enterobacter, Escherichia, Klebsiella, Flavobacterium and Weeksella species. The aerobic and facultatively anaerobic gram-negative rods and cocci isolation rate was high for irradiated individuals, and they were 64/25% and 100/80% for groups A/C and B/D, respectively. Recovery of aerobic and facultatively anaerobic gram-negative rods and cocci and Klebsiella pneumoniae subsp. pneumoniae in oral rinse samples were found to be significantly more prevalent in the irradiated subjects (groups A and B). Enterobacteriaceae were more frequently isolated from oral rinse samples of aged irradiated subjects (group B vs D, P<0.05), where the quantity of Citrobacter freundii (colony-forming units/ml oral rinse) was also significantly elevated. The isolation rate of aerobic and facultatively anaerobic gram-negative rods and cocci after hygienic care remained unchanged; 3 of 12 and 3 of 5 of the recalled subjects from groups A and B, respectively, harbored same aerobic and facultatively anaerobic gram-negative rods and cocci species. However, only two pairs of K. pneumoniae subsp. pneumoniae, sequentially isolated from same patients in group B, were found to be identical by pulsed-field gel electrophoresis.
Persistent Identifierhttp://hdl.handle.net/10722/157330
ISSN
2011 Impact Factor: 2.807
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, WKen_US
dc.contributor.authorJin, Len_US
dc.contributor.authorYam, WCen_US
dc.contributor.authorSamaranayake, LPen_US
dc.date.accessioned2012-08-08T08:48:58Z-
dc.date.available2012-08-08T08:48:58Z-
dc.date.issued2001en_US
dc.identifier.citationOral Microbiology and Immunology, 2001, v. 16 n. 1, p. 1-9en_US
dc.identifier.issn0902-0055en_US
dc.identifier.urihttp://hdl.handle.net/10722/157330-
dc.description.abstractThis study aimed at investigating the oral colonization of aerobic and facultatively anaerobic gram-negative rods and cocci in head- and neck-irradiated, dentate, xerostomic individuals. Subjects were recruited from a nasopharyngeal carcinoma clinic and were segregated into group A: <60 years (n=25, 48±6years, 5±5 years post-irradiation) and group B: ≥60 years (n=8, 67±4 years, 2±2 years post-irradiation) and were compared with age- and sex-matched normal individuals, group C: <60 years (n=20, 44±12 years) and group D: ≥60 years (n=10, 70±3 years). Selective culture of the oral rinse samples was carried out to isolate, quantify and speciate (using API 20E kit) aerobic and facultatively anaerobic gram-negative rods and cocci recovery. All test subjects were put under comprehensive oral and preventive care for 3 months, and 12 group A and 5 group B subjects were recalled for reassessment of aerobic and facultatively anaerobic gram-negative rods and cocci colonization. All identical isolates, pre- and post-hygienic care, were phenotypically (Vitek, Hazelwood, MA and antibiogram profile) and genotypically (pulsed-field gel electrophoresis) evaluated. The aerobic and facultatively anaerobic gram-negative rods and cocci isolated from the first round oral rinse samples included: Acinetobacter, Neisseria, Chryseomonas, Flavimonas, Pseudomonas, Citrobacter, Enterobacter, Escherichia, Klebsiella, Flavobacterium and Weeksella species. The aerobic and facultatively anaerobic gram-negative rods and cocci isolation rate was high for irradiated individuals, and they were 64/25% and 100/80% for groups A/C and B/D, respectively. Recovery of aerobic and facultatively anaerobic gram-negative rods and cocci and Klebsiella pneumoniae subsp. pneumoniae in oral rinse samples were found to be significantly more prevalent in the irradiated subjects (groups A and B). Enterobacteriaceae were more frequently isolated from oral rinse samples of aged irradiated subjects (group B vs D, P<0.05), where the quantity of Citrobacter freundii (colony-forming units/ml oral rinse) was also significantly elevated. The isolation rate of aerobic and facultatively anaerobic gram-negative rods and cocci after hygienic care remained unchanged; 3 of 12 and 3 of 5 of the recalled subjects from groups A and B, respectively, harbored same aerobic and facultatively anaerobic gram-negative rods and cocci species. However, only two pairs of K. pneumoniae subsp. pneumoniae, sequentially isolated from same patients in group B, were found to be identical by pulsed-field gel electrophoresis.en_US
dc.languageengen_US
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/OMIen_US
dc.relation.ispartofOral Microbiology and Immunologyen_US
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.subject.meshAcinetobacter - Classificationen_US
dc.subject.meshAdulten_US
dc.subject.meshAge Factorsen_US
dc.subject.meshAgeden_US
dc.subject.meshBacterial Typing Techniquesen_US
dc.subject.meshCarcinoma - Radiotherapyen_US
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshCitrobacter - Classificationen_US
dc.subject.meshCohort Studiesen_US
dc.subject.meshDna, Bacterial - Analysisen_US
dc.subject.meshDrug Resistance, Bacterialen_US
dc.subject.meshElectrophoresis, Gel, Pulsed-Fielden_US
dc.subject.meshEnterobacter - Classificationen_US
dc.subject.meshEscherichia - Classificationen_US
dc.subject.meshFlavobacterium - Classificationen_US
dc.subject.meshFollow-Up Studiesen_US
dc.subject.meshGenotypeen_US
dc.subject.meshGram-Negative Aerobic Rods And Cocci - Classificationen_US
dc.subject.meshGram-Negative Facultatively Anaerobic Rods - Classificationen_US
dc.subject.meshHumansen_US
dc.subject.meshKlebsiella - Classificationen_US
dc.subject.meshKlebsiella Pneumoniae - Classificationen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMouth - Microbiology - Radiation Effectsen_US
dc.subject.meshNasopharyngeal Neoplasms - Radiotherapyen_US
dc.subject.meshNeisseria - Classificationen_US
dc.subject.meshOral Hygieneen_US
dc.subject.meshPhenotypeen_US
dc.subject.meshPseudomonas - Classificationen_US
dc.subject.meshStatistics As Topicen_US
dc.subject.meshStatistics, Nonparametricen_US
dc.subject.meshXerostomia - Etiology - Microbiologyen_US
dc.titleOral colonization of aerobic and facultatively anaerobic gram-negative rods and cocci in irradiated, dentate, xerostomic individualsen_US
dc.typeArticleen_US
dc.identifier.emailLeung, WK: ewkleung@hkucc.hku.hken_US
dc.identifier.emailJin, L: ljjin@hkucc.hku.hken_US
dc.identifier.emailYam, WC: wcyam@hkucc.hku.hken_US
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_US
dc.identifier.authorityLeung, WK=rp00019en_US
dc.identifier.authorityJin, L=rp00028en_US
dc.identifier.authorityYam, WC=rp00313en_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1034/j.1399-302X.2001.160101.xen_US
dc.identifier.pmid11169132-
dc.identifier.scopuseid_2-s2.0-0035136771en_US
dc.identifier.hkuros58178-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035136771&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume16en_US
dc.identifier.issue1en_US
dc.identifier.spage1en_US
dc.identifier.epage9en_US
dc.identifier.isiWOS:000166189500001-
dc.publisher.placeDenmarken_US
dc.identifier.scopusauthoridLeung, WK=25224691800en_US
dc.identifier.scopusauthoridJin, LJ=7403328850en_US
dc.identifier.scopusauthoridYam, WC=7004281720en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US

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