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Article: Characterization of AFMP1: A novel target for serodiagnosis of Aspergillosis

TitleCharacterization of AFMP1: A novel target for serodiagnosis of Aspergillosis
Authors
Issue Date2001
Citation
Journal Of Clinical Microbiology, 2001, v. 39 n. 11, p. 3830-3837 How to Cite?
AbstractWe cloned the AFMP1 gene, which encodes the first antigenic cell wall galactomannoprotein in Aspergillus fumigatus. AFMP1 codes for a protein, Afmp1p, of 284 amino acid residues, with a few sequence features that are present in Mp1p, the antigenic cell wall mannoprotein in Penicillium marneffei that we described previously, as well as several other cell wall proteins of Saccharomyces cerevisiae and Candida albicans. It contains a serine- and threonine-rich region for O glycosylation, a signal peptide, and a putative glycosylphosphatidyl inositol attachment signal sequence. Specific anti-Afmp1p antibody was generated with recombinant Afmp1p protein purified from Escherichia coli to allow further characterization of Afmp1p. Afmp1p has a high affinity for Galanthus nivalis agglutinin, a characteristic indicative of a mannoprotein. Furthermore, it was recognized by a rat monoclonal antibody against the galactofuran side chain of galactomannan, indicating that it is a galactomannoprotein. Ultrastructural analysis by immunogold staining indicated that Afmp1p is present in the cell walls of the hyphae and conidia of A. fumigatus. Finally, it was observed that patients with aspergilloma and invasive aspergillosis due to A. fumigatus develop a specific antibody response against Afmp1p. This suggested that the recombinant protein and its antibody may be useful for serodiagnosis in patients with aspergilloma or invasive aspergillosis, and the protein may represent a good cell surface target for host humoral immunity.
Persistent Identifierhttp://hdl.handle.net/10722/157320
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYuen, KYen_US
dc.contributor.authorChan, CMen_US
dc.contributor.authorChan, KMen_US
dc.contributor.authorWoo, PCYen_US
dc.contributor.authorChe, XYen_US
dc.contributor.authorLeung, ASPen_US
dc.contributor.authorCao, Len_US
dc.date.accessioned2012-08-08T08:48:54Z-
dc.date.available2012-08-08T08:48:54Z-
dc.date.issued2001en_US
dc.identifier.citationJournal Of Clinical Microbiology, 2001, v. 39 n. 11, p. 3830-3837en_US
dc.identifier.issn0095-1137en_US
dc.identifier.urihttp://hdl.handle.net/10722/157320-
dc.description.abstractWe cloned the AFMP1 gene, which encodes the first antigenic cell wall galactomannoprotein in Aspergillus fumigatus. AFMP1 codes for a protein, Afmp1p, of 284 amino acid residues, with a few sequence features that are present in Mp1p, the antigenic cell wall mannoprotein in Penicillium marneffei that we described previously, as well as several other cell wall proteins of Saccharomyces cerevisiae and Candida albicans. It contains a serine- and threonine-rich region for O glycosylation, a signal peptide, and a putative glycosylphosphatidyl inositol attachment signal sequence. Specific anti-Afmp1p antibody was generated with recombinant Afmp1p protein purified from Escherichia coli to allow further characterization of Afmp1p. Afmp1p has a high affinity for Galanthus nivalis agglutinin, a characteristic indicative of a mannoprotein. Furthermore, it was recognized by a rat monoclonal antibody against the galactofuran side chain of galactomannan, indicating that it is a galactomannoprotein. Ultrastructural analysis by immunogold staining indicated that Afmp1p is present in the cell walls of the hyphae and conidia of A. fumigatus. Finally, it was observed that patients with aspergilloma and invasive aspergillosis due to A. fumigatus develop a specific antibody response against Afmp1p. This suggested that the recombinant protein and its antibody may be useful for serodiagnosis in patients with aspergilloma or invasive aspergillosis, and the protein may represent a good cell surface target for host humoral immunity.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Clinical Microbiologyen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAntibodies, Fungal - Blooden_US
dc.subject.meshAntigens, Fungal - Analysis - Genetics - Immunologyen_US
dc.subject.meshAspergillosis - Diagnosisen_US
dc.subject.meshAspergillus Fumigatus - Genetics - Immunology - Ultrastructureen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshHumansen_US
dc.subject.meshMembrane Glycoproteins - Analysis - Genetics - Immunologyen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshSequence Analysis, Dnaen_US
dc.subject.meshSerologic Testsen_US
dc.titleCharacterization of AFMP1: A novel target for serodiagnosis of Aspergillosisen_US
dc.typeArticleen_US
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_US
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.identifier.authorityWoo, PCY=rp00430en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1128/JCM.39.11.3830-3837.2001en_US
dc.identifier.pmid11682494-
dc.identifier.scopuseid_2-s2.0-0034758027en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034758027&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume39en_US
dc.identifier.issue11en_US
dc.identifier.spage3830en_US
dc.identifier.epage3837en_US
dc.identifier.isiWOS:000171934200003-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US
dc.identifier.scopusauthoridChan, CM=7404814453en_US
dc.identifier.scopusauthoridChan, KM=26324790600en_US
dc.identifier.scopusauthoridWoo, PCY=7201801340en_US
dc.identifier.scopusauthoridChe, XY=7005743182en_US
dc.identifier.scopusauthoridLeung, ASP=7403012546en_US
dc.identifier.scopusauthoridCao, L=7401637818en_US

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