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Article: Comparison of screening methods for detection of extended-spectrum β- lactamases and their prevalence among Escherichia coli and Klebsiella species in Hong Kong

TitleComparison of screening methods for detection of extended-spectrum β- lactamases and their prevalence among Escherichia coli and Klebsiella species in Hong Kong
Authors
Issue Date2000
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMIS
Citation
APMIS, 2000, v. 108 n. 3, p. 237-240 How to Cite?
AbstractThree tests, the disk diffusion test, the double-disc synergy test and the inhibitor-potentiated disc diffusion test, were compared for their abilities to detect production of extended-spectrum β-lactamases (ESBL) in 702 Escherichia coli and 472 Klebsiella spp. strains from four hospitals. Eleven percent E. coli and 13% Klebsiella spp. were found to produce ESBL. As an indicator of ESBL activity, the sensitivities of the five extended- spectrum β-lactams were as follows: cefotaxime (100%), cefpodoxime (99.3%), ceftriaxone (98.6%), aztreonam (93%) and ceftazidime (57.7%) when interpreted using the National Committee for Clinical Laboratory Standards criteria. Their positive predictive values ranged from 67.8-83.8%. Both the inhibitor- potentiated disc diffusion test and the double-disc synergy test (at three inter-disc widths of 20, 25 and 30 mm) were capable of identifying all the ESBL-producers. However, at a single inter-disc width of 30 mm, the double- disc synergy test has limited sensitivity (83.8%). As a second test for confirming ESBL activity in strains with reduced susceptibility to β- lactams, the inhibitor-potentiated disc diffusion test is therefore a simple and reliable option.
Persistent Identifierhttp://hdl.handle.net/10722/157314
ISSN
2015 Impact Factor: 1.933
2015 SCImago Journal Rankings: 0.855
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, PLen_US
dc.contributor.authorTsang, DNCen_US
dc.contributor.authorQue, TLen_US
dc.contributor.authorHo, Men_US
dc.contributor.authorYuen, KYen_US
dc.date.accessioned2012-08-08T08:48:51Z-
dc.date.available2012-08-08T08:48:51Z-
dc.date.issued2000en_US
dc.identifier.citationAPMIS, 2000, v. 108 n. 3, p. 237-240en_US
dc.identifier.issn0903-4641en_US
dc.identifier.urihttp://hdl.handle.net/10722/157314-
dc.description.abstractThree tests, the disk diffusion test, the double-disc synergy test and the inhibitor-potentiated disc diffusion test, were compared for their abilities to detect production of extended-spectrum β-lactamases (ESBL) in 702 Escherichia coli and 472 Klebsiella spp. strains from four hospitals. Eleven percent E. coli and 13% Klebsiella spp. were found to produce ESBL. As an indicator of ESBL activity, the sensitivities of the five extended- spectrum β-lactams were as follows: cefotaxime (100%), cefpodoxime (99.3%), ceftriaxone (98.6%), aztreonam (93%) and ceftazidime (57.7%) when interpreted using the National Committee for Clinical Laboratory Standards criteria. Their positive predictive values ranged from 67.8-83.8%. Both the inhibitor- potentiated disc diffusion test and the double-disc synergy test (at three inter-disc widths of 20, 25 and 30 mm) were capable of identifying all the ESBL-producers. However, at a single inter-disc width of 30 mm, the double- disc synergy test has limited sensitivity (83.8%). As a second test for confirming ESBL activity in strains with reduced susceptibility to β- lactams, the inhibitor-potentiated disc diffusion test is therefore a simple and reliable option.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMISen_US
dc.relation.ispartofAPMISen_US
dc.subject.meshEscherichia Coli - Enzymology - Growth & Developmenten_US
dc.subject.meshHong Kongen_US
dc.subject.meshHumansen_US
dc.subject.meshKlebsiella - Enzymology - Growth & Developmenten_US
dc.subject.meshBeta-Lactamases - Analysisen_US
dc.titleComparison of screening methods for detection of extended-spectrum β- lactamases and their prevalence among Escherichia coli and Klebsiella species in Hong Kongen_US
dc.typeArticleen_US
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_US
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_US
dc.identifier.authorityHo, PL=rp00406en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1034/j.1600-0463.2000.d01-50.x-
dc.identifier.pmid10752694-
dc.identifier.scopuseid_2-s2.0-0034058229en_US
dc.identifier.hkuros50543-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034058229&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume108en_US
dc.identifier.issue3en_US
dc.identifier.spage237en_US
dc.identifier.epage240en_US
dc.identifier.isiWOS:000086041700011-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHo, PL=7402211363en_US
dc.identifier.scopusauthoridTsang, DNC=7005609132en_US
dc.identifier.scopusauthoridQue, TL=7003786628en_US
dc.identifier.scopusauthoridHo, M=7403080752en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US

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