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Article: Enhancement of humoral and protective immune response induced by live- attenuated Salmonella typhi by ampicillin

TitleEnhancement of humoral and protective immune response induced by live- attenuated Salmonella typhi by ampicillin
Authors
Issue Date2000
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMIS
Citation
Apmis, 2000, v. 108 n. 5, p. 343-348 How to Cite?
AbstractEfficacy of live-attenuated Salmonella vaccines delivered by the mucosal route is limited by the dose and interference from mucosal flora of the alimentary tract. In a mouse model, the total antibody response towards lipopolysaccharide of S. typhi was significantly enhanced at day 21 post- immunization with live-attenuated S. typhi (Ty21a) when ampicillin was concomitantly administered (p<0.005), and the lethal dose 50 of mice in the ampicillin and control groups immunized with Ty21a after wild-type S. typhi challenge on day 24 was 4 x 107 and 1 x 107, respectively. The faecal bacterial counts of the ampicillin group at days 1 and 3 were significantly lower than those of the control group (p<0.01 and <0.05). On day 1, the number of mice with ≥10 Ty21a colonies isolated from the spleen was significantly higher in the ampicillin group than the control group (p<0.05). Furthermore, on the same day, Ty21a was isolated from the faeces of three mice from the ampicillin group, but only one from the control group. We conclude that ampicillin may have enhanced the humoral and protective immune responses by giving the Ty21a a selective advantage over the normal bacterial flora. This concept of antibiotic enhancement of immunization could have important implications for other live-attenuated vaccines, as well as the delivery of microbial antigens and DNA vaccines by live-attenuated Salmonella carriers.
Persistent Identifierhttp://hdl.handle.net/10722/157309
ISSN
2015 Impact Factor: 1.933
2015 SCImago Journal Rankings: 0.855
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_US
dc.contributor.authorTsoi, HWen_US
dc.contributor.authorLeung, HCHen_US
dc.contributor.authorYuen, KYen_US
dc.date.accessioned2012-08-08T08:48:49Z-
dc.date.available2012-08-08T08:48:49Z-
dc.date.issued2000en_US
dc.identifier.citationApmis, 2000, v. 108 n. 5, p. 343-348en_US
dc.identifier.issn0903-4641en_US
dc.identifier.urihttp://hdl.handle.net/10722/157309-
dc.description.abstractEfficacy of live-attenuated Salmonella vaccines delivered by the mucosal route is limited by the dose and interference from mucosal flora of the alimentary tract. In a mouse model, the total antibody response towards lipopolysaccharide of S. typhi was significantly enhanced at day 21 post- immunization with live-attenuated S. typhi (Ty21a) when ampicillin was concomitantly administered (p<0.005), and the lethal dose 50 of mice in the ampicillin and control groups immunized with Ty21a after wild-type S. typhi challenge on day 24 was 4 x 107 and 1 x 107, respectively. The faecal bacterial counts of the ampicillin group at days 1 and 3 were significantly lower than those of the control group (p<0.01 and <0.05). On day 1, the number of mice with ≥10 Ty21a colonies isolated from the spleen was significantly higher in the ampicillin group than the control group (p<0.05). Furthermore, on the same day, Ty21a was isolated from the faeces of three mice from the ampicillin group, but only one from the control group. We conclude that ampicillin may have enhanced the humoral and protective immune responses by giving the Ty21a a selective advantage over the normal bacterial flora. This concept of antibiotic enhancement of immunization could have important implications for other live-attenuated vaccines, as well as the delivery of microbial antigens and DNA vaccines by live-attenuated Salmonella carriers.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMISen_US
dc.relation.ispartofAPMISen_US
dc.subject.meshAmpicillin - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Bacterial - Blooden_US
dc.subject.meshBacterial Vaccines - Immunologyen_US
dc.subject.meshColony Count, Microbialen_US
dc.subject.meshFeces - Microbiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshLipopolysaccharides - Immunologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Balb Cen_US
dc.subject.meshPenicillins - Pharmacologyen_US
dc.subject.meshSalmonella Typhi - Immunologyen_US
dc.titleEnhancement of humoral and protective immune response induced by live- attenuated Salmonella typhi by ampicillinen_US
dc.typeArticleen_US
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_US
dc.identifier.emailTsoi, HW:hwtsoi@hkucc.hku.hken_US
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_US
dc.identifier.authorityWoo, PCY=rp00430en_US
dc.identifier.authorityTsoi, HW=rp00439en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid10937771-
dc.identifier.scopuseid_2-s2.0-0033920062en_US
dc.identifier.hkuros54282-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033920062&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume108en_US
dc.identifier.issue5en_US
dc.identifier.spage343en_US
dc.identifier.epage348en_US
dc.identifier.isiWOS:000088451500005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWoo, PCY=7201801340en_US
dc.identifier.scopusauthoridTsoi, HW=6603822102en_US
dc.identifier.scopusauthoridLeung, HCH=36755846800en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US

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