File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Expression of Epstein-Barr virus BamHI-A rightward transcripts in latently infected B cells from peripheral blood

TitleExpression of Epstein-Barr virus BamHI-A rightward transcripts in latently infected B cells from peripheral blood
Authors
Issue Date1999
PublisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
Citation
Blood, 1999, v. 93 n. 9, p. 3026-3032 How to Cite?
AbstractIn addition to the Epstein-Barr virus (EBV) EBNA and LMP latency genes, there is a family of alternatively spliced BamHI-A rightward transcripts (BARTs). These latency transcripts are highly expressed in the EBV-associated malignancies nasopharyngeal carcinoma and Burkitt's lymphoma, and are expressed at lower levels in latently EBV-infected B-cell lines. The contribution of the BARTs to EBV biology or pathogenesis is unknown. Resting B cells have recently been recognized as a reservoir for EBV persistence in the peripheral blood. In these cells, EBV gene expression is tightly restricted and the only viral gene known to be consistently expressed is LMP2A. We used cell sorting and reverse-transcriptase polymerase chain reaction (RT-PCR) to examine whether BARTs are expressed in the restricted form of in vivo latency. Our results demonstrated that RNAs with splicing diagnostic for transcripts containing the BART RPMS1 and BARFO open-reading frames (ORFs) were expressed in CD19+ but not in CD23+ B cells isolated from peripheral blood of healthy individuals. The product of the proximal RPMS1 ORF has not previously been characterized. The RPMS1 ORF was shown to encode a 15-kD protein that localized to the nucleus of transfected cells. Expression of the BARTs in peripheral blood B cells suggests that the proteins encoded by these transcripts are likely to be important for maintenance of in vivo latency.
Persistent Identifierhttp://hdl.handle.net/10722/157298
ISSN
2015 Impact Factor: 11.841
2015 SCImago Journal Rankings: 6.395
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Hen_US
dc.contributor.authorSmith, Pen_US
dc.contributor.authorAmbinder, RFen_US
dc.contributor.authorHayward, SDen_US
dc.date.accessioned2012-08-08T08:48:45Z-
dc.date.available2012-08-08T08:48:45Z-
dc.date.issued1999en_US
dc.identifier.citationBlood, 1999, v. 93 n. 9, p. 3026-3032en_US
dc.identifier.issn0006-4971en_US
dc.identifier.urihttp://hdl.handle.net/10722/157298-
dc.description.abstractIn addition to the Epstein-Barr virus (EBV) EBNA and LMP latency genes, there is a family of alternatively spliced BamHI-A rightward transcripts (BARTs). These latency transcripts are highly expressed in the EBV-associated malignancies nasopharyngeal carcinoma and Burkitt's lymphoma, and are expressed at lower levels in latently EBV-infected B-cell lines. The contribution of the BARTs to EBV biology or pathogenesis is unknown. Resting B cells have recently been recognized as a reservoir for EBV persistence in the peripheral blood. In these cells, EBV gene expression is tightly restricted and the only viral gene known to be consistently expressed is LMP2A. We used cell sorting and reverse-transcriptase polymerase chain reaction (RT-PCR) to examine whether BARTs are expressed in the restricted form of in vivo latency. Our results demonstrated that RNAs with splicing diagnostic for transcripts containing the BART RPMS1 and BARFO open-reading frames (ORFs) were expressed in CD19+ but not in CD23+ B cells isolated from peripheral blood of healthy individuals. The product of the proximal RPMS1 ORF has not previously been characterized. The RPMS1 ORF was shown to encode a 15-kD protein that localized to the nucleus of transfected cells. Expression of the BARTs in peripheral blood B cells suggests that the proteins encoded by these transcripts are likely to be important for maintenance of in vivo latency.en_US
dc.languageengen_US
dc.publisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/en_US
dc.relation.ispartofBlooden_US
dc.subject.meshAntigens, Cd - Analysisen_US
dc.subject.meshAntigens, Cd19 - Analysisen_US
dc.subject.meshB-Lymphocytes - Immunology - Virologyen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBurkitt Lymphoma - Geneticsen_US
dc.subject.meshDna Primersen_US
dc.subject.meshDeoxyribonuclease Bamhien_US
dc.subject.meshEpstein-Barr Virus Nuclear Antigens - Geneticsen_US
dc.subject.meshGene Expression Regulation, Viralen_US
dc.subject.meshHerpesvirus 4, Human - Genetics - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOpen Reading Framesen_US
dc.subject.meshRestriction Mappingen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSequence Alignmenten_US
dc.subject.meshSequence Homology, Nucleic Aciden_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshViral Matrix Proteins - Geneticsen_US
dc.subject.meshVirus Latency - Geneticsen_US
dc.titleExpression of Epstein-Barr virus BamHI-A rightward transcripts in latently infected B cells from peripheral blooden_US
dc.typeArticleen_US
dc.identifier.emailChen, H:hlchen@hkucc.hku.hken_US
dc.identifier.authorityChen, H=rp00383en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid10216099-
dc.identifier.scopuseid_2-s2.0-0033134768en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033134768&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume93en_US
dc.identifier.issue9en_US
dc.identifier.spage3026en_US
dc.identifier.epage3032en_US
dc.identifier.isiWOS:000079984800032-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChen, H=26643315400en_US
dc.identifier.scopusauthoridSmith, P=7406997818en_US
dc.identifier.scopusauthoridAmbinder, RF=7005598325en_US
dc.identifier.scopusauthoridHayward, SD=7102776214en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats