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Article: Transcription units of E1a, E1b and pIX regions of bovine adenovirus type 3

TitleTranscription units of E1a, E1b and pIX regions of bovine adenovirus type 3
Authors
Issue Date1999
PublisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org
Citation
Journal Of General Virology, 1999, v. 80 n. 7, p. 1735-1742 How to Cite?
AbstractThe major mRNA species in the E1 region of the genome of bovine adenovirus type 3 (BAV3) have been defined by using a combination of PCR, 5' RACE, Northern analysis and DNA sequencing. Independent transcription initiation sites were identified for each of the E1a, E1b and protein IX (pIX) transcription units, but all mRNA species terminated at the same poly(A) addition site immediately downstream of the pIX open reading frame. Thus, the BAV3 E1 region, which consists of the E1a and E1b genes together with that for pIX, functions as a nested overlapping transcription unit. One major mRNA species encoding the E1a protein was found and two mRNAs encoding E1b species, the smaller of which encodes the E1b 17K protein alone and the larger encodes both 17K and 47K E1b proteins, were identified. One mRNA species encodes pIX. The E1a transcript, encoding the predicted 214 residue E1a protein, has four exons. The smaller E1b mRNA has two exons, the second of which corresponds to the last exon of E1a. No introns were detected in the larger E1b mRNA that encodes both the E1b 17K and 47K proteins nor in the mRNA encoding pIX. The relative times of appearance of the mRNAs from the E1-pIX gene region following infection of bovine cells with BAV3 was determined.
Persistent Identifierhttp://hdl.handle.net/10722/157297
ISSN
2015 Impact Factor: 3.192
2015 SCImago Journal Rankings: 1.741
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZheng, BJen_US
dc.contributor.authorGraham, FLen_US
dc.contributor.authorPrevec, Len_US
dc.date.accessioned2012-08-08T08:48:44Z-
dc.date.available2012-08-08T08:48:44Z-
dc.date.issued1999en_US
dc.identifier.citationJournal Of General Virology, 1999, v. 80 n. 7, p. 1735-1742en_US
dc.identifier.issn0022-1317en_US
dc.identifier.urihttp://hdl.handle.net/10722/157297-
dc.description.abstractThe major mRNA species in the E1 region of the genome of bovine adenovirus type 3 (BAV3) have been defined by using a combination of PCR, 5' RACE, Northern analysis and DNA sequencing. Independent transcription initiation sites were identified for each of the E1a, E1b and protein IX (pIX) transcription units, but all mRNA species terminated at the same poly(A) addition site immediately downstream of the pIX open reading frame. Thus, the BAV3 E1 region, which consists of the E1a and E1b genes together with that for pIX, functions as a nested overlapping transcription unit. One major mRNA species encoding the E1a protein was found and two mRNAs encoding E1b species, the smaller of which encodes the E1b 17K protein alone and the larger encodes both 17K and 47K E1b proteins, were identified. One mRNA species encodes pIX. The E1a transcript, encoding the predicted 214 residue E1a protein, has four exons. The smaller E1b mRNA has two exons, the second of which corresponds to the last exon of E1a. No introns were detected in the larger E1b mRNA that encodes both the E1b 17K and 47K proteins nor in the mRNA encoding pIX. The relative times of appearance of the mRNAs from the E1-pIX gene region following infection of bovine cells with BAV3 was determined.en_US
dc.languageengen_US
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.orgen_US
dc.relation.ispartofJournal of General Virologyen_US
dc.subject.meshAdenovirus E1a Proteins - Geneticsen_US
dc.subject.meshAdenovirus E1b Proteins - Geneticsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCattleen_US
dc.subject.meshGenome, Viralen_US
dc.subject.meshMastadenovirus - Geneticsen_US
dc.subject.meshOpen Reading Frames - Geneticsen_US
dc.subject.meshRna, Messenger - Analysis - Geneticsen_US
dc.subject.meshRna, Viral - Analysis - Geneticsen_US
dc.subject.meshTranscription, Geneticen_US
dc.titleTranscription units of E1a, E1b and pIX regions of bovine adenovirus type 3en_US
dc.typeArticleen_US
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_US
dc.identifier.authorityZheng, BJ=rp00353en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid10423142-
dc.identifier.scopuseid_2-s2.0-0032987014en_US
dc.identifier.hkuros46294-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032987014&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume80en_US
dc.identifier.issue7en_US
dc.identifier.spage1735en_US
dc.identifier.epage1742en_US
dc.identifier.isiWOS:000081303900021-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridZheng, BJ=7201780588en_US
dc.identifier.scopusauthoridGraham, FL=35821642700en_US
dc.identifier.scopusauthoridPrevec, L=7003455531en_US

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