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Article: Hot spot mutations in morphological transforming region II (ORF 79) of cytomegalovirus strains causing disease from bone marrow transplant recipients

TitleHot spot mutations in morphological transforming region II (ORF 79) of cytomegalovirus strains causing disease from bone marrow transplant recipients
Authors
Issue Date1999
PublisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705
Citation
Archives of Virology, 1999, v. 144 n. 3, p. 601-612 How to Cite?
AbstractNested polymerase chain reaction (PCR) amplifying the morphological transforming region II (mtrII) of cytomegalovirus (CMV) has been shown to be useful in the detection of CMV DNA in bone marrow transplant (BMT) recipients. However, there has never been any report on mutation hot spots and subtypes of this open reading frame. Using primers derived from sequences upstream and downstream of mtrII (ORF 79), CMV DNA from peripheral blood leukocytes (PBL) and conventional CMV culture of 16 BMT recipients were amplified by PCR, cloned into pUC 118, and sequenced. The amino acid sequences were predicted using the standard triplet code. The DNA sequences obtained from direct amplification of CMV in PBL obtained from the 16 patients were 100% identical to the corresponding ones obtained by amplification of CMV DNA extracted from conventional CMV culture. Within mtrII (ORF 79), hot spot single base mutations were observed at positions +40 (G→A), +123 (A→G), +213 (T→C), and +219 (T→C). However, because of third base degeneracy, only amino acid 14 was changed from valine to isoleucine in the predicted protein of 13 patients. This corresponded to the hot spot mutation at position +40 (GTC→ATC), while the rest were silent mutations. An insertion of 3 bases (ACG) was observed in the CMV DNA of 10 patients at positions +91 to +93, leading to a threonine insertion at amino acid 31 in these patients. For patient no. 147 there was a 65 bp deletion in the CMV DNA amplified later in the course of BMT as compared with that early in the course. This gave rise to a frame shift mutation and a change of more than 70% in the predicted amino acid sequence of the protein.
Persistent Identifierhttp://hdl.handle.net/10722/157295
ISSN
2015 Impact Factor: 2.255
2015 SCImago Journal Rankings: 1.086
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLo, SKFen_US
dc.contributor.authorWoo, PCYen_US
dc.contributor.authorYuen, KYen_US
dc.date.accessioned2012-08-08T08:48:43Z-
dc.date.available2012-08-08T08:48:43Z-
dc.date.issued1999en_US
dc.identifier.citationArchives of Virology, 1999, v. 144 n. 3, p. 601-612en_US
dc.identifier.issn0304-8608en_US
dc.identifier.urihttp://hdl.handle.net/10722/157295-
dc.description.abstractNested polymerase chain reaction (PCR) amplifying the morphological transforming region II (mtrII) of cytomegalovirus (CMV) has been shown to be useful in the detection of CMV DNA in bone marrow transplant (BMT) recipients. However, there has never been any report on mutation hot spots and subtypes of this open reading frame. Using primers derived from sequences upstream and downstream of mtrII (ORF 79), CMV DNA from peripheral blood leukocytes (PBL) and conventional CMV culture of 16 BMT recipients were amplified by PCR, cloned into pUC 118, and sequenced. The amino acid sequences were predicted using the standard triplet code. The DNA sequences obtained from direct amplification of CMV in PBL obtained from the 16 patients were 100% identical to the corresponding ones obtained by amplification of CMV DNA extracted from conventional CMV culture. Within mtrII (ORF 79), hot spot single base mutations were observed at positions +40 (G→A), +123 (A→G), +213 (T→C), and +219 (T→C). However, because of third base degeneracy, only amino acid 14 was changed from valine to isoleucine in the predicted protein of 13 patients. This corresponded to the hot spot mutation at position +40 (GTC→ATC), while the rest were silent mutations. An insertion of 3 bases (ACG) was observed in the CMV DNA of 10 patients at positions +91 to +93, leading to a threonine insertion at amino acid 31 in these patients. For patient no. 147 there was a 65 bp deletion in the CMV DNA amplified later in the course of BMT as compared with that early in the course. This gave rise to a frame shift mutation and a change of more than 70% in the predicted amino acid sequence of the protein.en_US
dc.languageengen_US
dc.publisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705en_US
dc.relation.ispartofArchives of Virologyen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBone Marrow Transplantationen_US
dc.subject.meshCell Transformation, Viralen_US
dc.subject.meshCytomegalovirus - Geneticsen_US
dc.subject.meshCytomegalovirus Infections - Virologyen_US
dc.subject.meshDna, Viralen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMutationen_US
dc.subject.meshOncogene Proteins - Geneticsen_US
dc.subject.meshOncogene Proteins, Viralen_US
dc.subject.meshSequence Homology, Nucleic Aciden_US
dc.subject.meshViral Proteins - Geneticsen_US
dc.titleHot spot mutations in morphological transforming region II (ORF 79) of cytomegalovirus strains causing disease from bone marrow transplant recipientsen_US
dc.typeArticleen_US
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hken_US
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_US
dc.identifier.authorityWoo, PCY=rp00430en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s007050050528en_US
dc.identifier.pmid10226623-
dc.identifier.scopuseid_2-s2.0-0032933148en_US
dc.identifier.hkuros46194-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032933148&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume144en_US
dc.identifier.issue3en_US
dc.identifier.spage601en_US
dc.identifier.epage612en_US
dc.identifier.isiWOS:000079232400013-
dc.publisher.placeAustriaen_US
dc.identifier.scopusauthoridLo, SKF=7401542391en_US
dc.identifier.scopusauthoridWoo, PCY=7201801340en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US
dc.customcontrol.immutablesml 130530-

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