File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: An evaluation of susceptibility testing methods for ampicillin-sulbactam using a panel of β-lactamase-producing bacteria

TitleAn evaluation of susceptibility testing methods for ampicillin-sulbactam using a panel of β-lactamase-producing bacteria
Authors
Issue Date1999
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMIS
Citation
Apmis, 1999, v. 107 n. 7, p. 703-708 How to Cite?
AbstractBacteria possessing TEM-1-like β-lactamases are generally regarded as susceptible to ampicillin-sulbactam (SAM), while those harboring OXA-1 enzymes are considered resistant. The current study was undertaken to compare susceptibility testing using various combinations of ampicillin and sulbactam to improve clinical correlation. Members of the Enterobacteriaceae family harboring TEM-1, SHV-1 or OXA-1-like β-lactamases were tested using the agar dilution method. A substantial proportion of strains harboring OXA-1-like β- lactamases showed false susceptibility to SAM at the 1:1 ratio or fixed sulbactam concentration of 8 μg/ml. At a fixed sulbactam concentration of 4 μg/ml, the activity of ampicillin-sulbactam appeared to be reduced, with large numbers of TEM-1 producers becoming frankly resistant. Results obtained with the 2:1 ratio exhibited the closest correlation with that obtained by the currently recommended disk diffusion test. However, very major errors were still found between the disk diffusion test and agar dilution test, suggesting the necessity for consideration of a change in criteria for interpretation of disk diffusion test results. In conclusion, SAM susceptibility testing by agar dilution using other than a 2:1 ratio is not recommended and results should be interpreted with caution.
Persistent Identifierhttp://hdl.handle.net/10722/157293
ISSN
2015 Impact Factor: 1.933
2015 SCImago Journal Rankings: 0.855
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSiu, LKen_US
dc.contributor.authorLo, JYCen_US
dc.contributor.authorCheng, WLen_US
dc.contributor.authorHo, PLen_US
dc.contributor.authorNg, WSen_US
dc.contributor.authorChau, PYen_US
dc.date.accessioned2012-08-08T08:48:43Z-
dc.date.available2012-08-08T08:48:43Z-
dc.date.issued1999en_US
dc.identifier.citationApmis, 1999, v. 107 n. 7, p. 703-708en_US
dc.identifier.issn0903-4641en_US
dc.identifier.urihttp://hdl.handle.net/10722/157293-
dc.description.abstractBacteria possessing TEM-1-like β-lactamases are generally regarded as susceptible to ampicillin-sulbactam (SAM), while those harboring OXA-1 enzymes are considered resistant. The current study was undertaken to compare susceptibility testing using various combinations of ampicillin and sulbactam to improve clinical correlation. Members of the Enterobacteriaceae family harboring TEM-1, SHV-1 or OXA-1-like β-lactamases were tested using the agar dilution method. A substantial proportion of strains harboring OXA-1-like β- lactamases showed false susceptibility to SAM at the 1:1 ratio or fixed sulbactam concentration of 8 μg/ml. At a fixed sulbactam concentration of 4 μg/ml, the activity of ampicillin-sulbactam appeared to be reduced, with large numbers of TEM-1 producers becoming frankly resistant. Results obtained with the 2:1 ratio exhibited the closest correlation with that obtained by the currently recommended disk diffusion test. However, very major errors were still found between the disk diffusion test and agar dilution test, suggesting the necessity for consideration of a change in criteria for interpretation of disk diffusion test results. In conclusion, SAM susceptibility testing by agar dilution using other than a 2:1 ratio is not recommended and results should be interpreted with caution.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMISen_US
dc.relation.ispartofAPMISen_US
dc.subject.meshAgaren_US
dc.subject.meshAmpicillin - Pharmacologyen_US
dc.subject.meshAnti-Bacterial Agents - Pharmacologyen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshDrug Evaluationen_US
dc.subject.meshEscherichia Coli - Drug Effectsen_US
dc.subject.meshHumansen_US
dc.subject.meshKlebsiella - Drug Effectsen_US
dc.subject.meshMicrobial Sensitivity Testsen_US
dc.subject.meshShigella - Drug Effectsen_US
dc.subject.meshSulbactam - Pharmacologyen_US
dc.subject.meshBeta-Lactamasesen_US
dc.titleAn evaluation of susceptibility testing methods for ampicillin-sulbactam using a panel of β-lactamase-producing bacteriaen_US
dc.typeArticleen_US
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_US
dc.identifier.authorityHo, PL=rp00406en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid10440070-
dc.identifier.scopuseid_2-s2.0-0032813395en_US
dc.identifier.hkuros50549-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032813395&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume107en_US
dc.identifier.issue7en_US
dc.identifier.spage703en_US
dc.identifier.epage708en_US
dc.identifier.isiWOS:000081697300015-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSiu, LK=7006651154en_US
dc.identifier.scopusauthoridLo, JYC=7201650939en_US
dc.identifier.scopusauthoridCheng, WL=20633800000en_US
dc.identifier.scopusauthoridHo, PL=7402211363en_US
dc.identifier.scopusauthoridNg, WS=36787042500en_US
dc.identifier.scopusauthoridChau, PY=36509704300en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats