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Article: Primary SIVsm isolates use the CCR5 coreceptor from sooty mangabeys naturally infected in West Africa: A comparison of coreceptor usage of primary SIVsm, HIV-2, and SIVmac

TitlePrimary SIVsm isolates use the CCR5 coreceptor from sooty mangabeys naturally infected in West Africa: A comparison of coreceptor usage of primary SIVsm, HIV-2, and SIVmac
Authors
Issue Date1998
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
Citation
Virology, 1998, v. 246 n. 1, p. 113-124 How to Cite?
AbstractGenetically divergent strains of simian immunodeficiency virus (SIV) from macaques (mac), chimpanzees, and sooty mangabeys (SM) efficiently used rhesus and human CCR5 (R5), but not CXCR4 (xR4), for cell entry. Thus far, however, no studies have characterized primary SIVsm strains for their use of coreceptors derived from their own natural host. Coreceptor usage of two primary, blood-derived SIVsm isolates, SIVsmSL92b and SIVsmFNS from naturally infected sooty mangabeys, was determined. Primary SIVsm efficiently used SM- CCR5 expressed on HOS.CD4 and U87.CD4 cells. Sequence polymorphisms in CCR5 found in four sooty mangabeys did not alter viral entry. Unlike primary rhesus blood-derived R5-tropic SIVmac251, primary SM blood-derived R5-tropic SIVsm was strongly CD4 dependent. The SM-CXCR4 gene was fully functional for xR4-tropic primate lentiviruses, but was not used by primary SIVsm. Therefore, the lack of xR4 tropism among naturally occurring SIVsm strains was not due to CxCR4 gene defects in the natural host. SIVmac derived from four macaques with AIDS also did not use macaque- or SM-derived CXCR4, showing that xR4 tropism did not develop during progression to disease as for humans infected with HIV-1. Three of four primary HIV-2 strains used CCR5 from human, sooty mangabey, and macaque. The fourth, HIV-27924A, obtained from a patient with AIDS, was xR4-tropic. Because SIVmac is most closely related to HIV-2, SIVmac might be expected to mimic tropisms of HIV-2 infections. However, the correlation between xR4 tropism and AIDS may be a species-specific phenomenon limited to humans. The R5-tropic primary SIVsm and HIV-2 strains grew in CCR5-negative human PBMC, consistent with their use of non-CCR5 coreceptors. However, primary SIVsmSL92b did not use non-CCR5 coreceptors efficiently. The two primary SIVsm isolates replicated poorly in CEMx174 cells, which do not express CCR5, compared to CCR5-positive PM1 cells. SIVmac grew equally well in both cell lines. The findings show that SM-chemokine receptors are fully functional for virus entry and that multicoreceptor tropism is a common property of primary lentiviruses within the SIVsm/HIV-2 subfamily.
Persistent Identifierhttp://hdl.handle.net/10722/157291
ISSN
2015 Impact Factor: 3.2
2015 SCImago Journal Rankings: 1.805
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Zen_US
dc.contributor.authorGettie, Aen_US
dc.contributor.authorHo, DDen_US
dc.contributor.authorMarx, PAen_US
dc.date.accessioned2012-08-08T08:48:42Z-
dc.date.available2012-08-08T08:48:42Z-
dc.date.issued1998en_US
dc.identifier.citationVirology, 1998, v. 246 n. 1, p. 113-124en_US
dc.identifier.issn0042-6822en_US
dc.identifier.urihttp://hdl.handle.net/10722/157291-
dc.description.abstractGenetically divergent strains of simian immunodeficiency virus (SIV) from macaques (mac), chimpanzees, and sooty mangabeys (SM) efficiently used rhesus and human CCR5 (R5), but not CXCR4 (xR4), for cell entry. Thus far, however, no studies have characterized primary SIVsm strains for their use of coreceptors derived from their own natural host. Coreceptor usage of two primary, blood-derived SIVsm isolates, SIVsmSL92b and SIVsmFNS from naturally infected sooty mangabeys, was determined. Primary SIVsm efficiently used SM- CCR5 expressed on HOS.CD4 and U87.CD4 cells. Sequence polymorphisms in CCR5 found in four sooty mangabeys did not alter viral entry. Unlike primary rhesus blood-derived R5-tropic SIVmac251, primary SM blood-derived R5-tropic SIVsm was strongly CD4 dependent. The SM-CXCR4 gene was fully functional for xR4-tropic primate lentiviruses, but was not used by primary SIVsm. Therefore, the lack of xR4 tropism among naturally occurring SIVsm strains was not due to CxCR4 gene defects in the natural host. SIVmac derived from four macaques with AIDS also did not use macaque- or SM-derived CXCR4, showing that xR4 tropism did not develop during progression to disease as for humans infected with HIV-1. Three of four primary HIV-2 strains used CCR5 from human, sooty mangabey, and macaque. The fourth, HIV-27924A, obtained from a patient with AIDS, was xR4-tropic. Because SIVmac is most closely related to HIV-2, SIVmac might be expected to mimic tropisms of HIV-2 infections. However, the correlation between xR4 tropism and AIDS may be a species-specific phenomenon limited to humans. The R5-tropic primary SIVsm and HIV-2 strains grew in CCR5-negative human PBMC, consistent with their use of non-CCR5 coreceptors. However, primary SIVsmSL92b did not use non-CCR5 coreceptors efficiently. The two primary SIVsm isolates replicated poorly in CEMx174 cells, which do not express CCR5, compared to CCR5-positive PM1 cells. SIVmac grew equally well in both cell lines. The findings show that SM-chemokine receptors are fully functional for virus entry and that multicoreceptor tropism is a common property of primary lentiviruses within the SIVsm/HIV-2 subfamily.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviroen_US
dc.relation.ispartofVirologyen_US
dc.subject.meshAcquired Immunodeficiency Syndrome - Virologyen_US
dc.subject.meshAfrica, Westernen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCd4-Positive T-Lymphocytesen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCercocebus Atys - Virologyen_US
dc.subject.meshGenes - Geneticsen_US
dc.subject.meshHiv-2 - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMacacaen_US
dc.subject.meshMacaca Mulattaen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPolymorphism, Geneticen_US
dc.subject.meshReceptors, Ccr5 - Genetics - Physiologyen_US
dc.subject.meshReceptors, Cxcr4 - Genetics - Physiologyen_US
dc.subject.meshReceptors, Hiv - Physiologyen_US
dc.subject.meshReceptors, Virus - Genetics - Physiologyen_US
dc.subject.meshSimian Acquired Immunodeficiency Syndrome - Virologyen_US
dc.subject.meshSimian Immunodeficiency Virus - Isolation & Purification - Physiologyen_US
dc.subject.meshVirus Replicationen_US
dc.titlePrimary SIVsm isolates use the CCR5 coreceptor from sooty mangabeys naturally infected in West Africa: A comparison of coreceptor usage of primary SIVsm, HIV-2, and SIVmacen_US
dc.typeArticleen_US
dc.identifier.emailChen, Z:zchenai@hkucc.hku.hken_US
dc.identifier.authorityChen, Z=rp00243en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/viro.1998.9174en_US
dc.identifier.pmid9656999-
dc.identifier.scopuseid_2-s2.0-0032551184en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032551184&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume246en_US
dc.identifier.issue1en_US
dc.identifier.spage113en_US
dc.identifier.epage124en_US
dc.identifier.isiWOS:000074462500011-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChen, Z=35271180800en_US
dc.identifier.scopusauthoridGettie, A=7003730114en_US
dc.identifier.scopusauthoridHo, DD=7402971998en_US
dc.identifier.scopusauthoridMarx, PA=7102894750en_US

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