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Article: Immunogenicity in mice of tandem repeats of an epitope from herpes simplex gD protein when expressed by recombinant adenovirus vectors

TitleImmunogenicity in mice of tandem repeats of an epitope from herpes simplex gD protein when expressed by recombinant adenovirus vectors
Authors
Issue Date1993
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccine
Citation
Vaccine, 1993, v. 11 n. 12, p. 1191-1197 How to Cite?
AbstractThe antigenic and immunogenic potential was examined of human adenovirus type 5 (Ad) recombinants carrying and expressing from one to four tandem repeats of a linear neutralizing epitope from the gD protein of herpes simplex virus type 1 (HSV-1) as a fusion with the β-galactosidase protein. The fusion proteins produced by these Ad vectors in infected cell culture reacted with a herpes simplex virus (HSV) epitope-specific monoclonal antibody to a degree dependent on the number of epitope repeats in the protein. Mice immunized by intraperitoneal injection of the Ad vectors developed an anti-HSV immune response as measured by ELISA and by HSV-1 neutralization assays. The mean antibody titre induced by a single injection of the Ad vector increased with the number of epitope repeats expressed by the recombinant. Any animal that had developed a serum-neutralizing titre of at least 1:80 survived challenge with a normally lethal dose of HSV-2 administered by the intraperitoneal route. Recombinant vectors expressing four repeats of the HSV epitope were as effective in antibody induction and protection as an adenovirus vector carrying and expressing the entire HSV gD protein. These results suggest that the expression of tandem repeats of appropriate epitopic sequences by adenovirus vectors may provide a safe and effective method of immunizing against HSV infection.
Persistent Identifierhttp://hdl.handle.net/10722/157261
ISSN
2015 Impact Factor: 3.413
2015 SCImago Journal Rankings: 2.044
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZheng, Ben_US
dc.contributor.authorGraham, FLen_US
dc.contributor.authorJohnson, DCen_US
dc.contributor.authorHanke, Ten_US
dc.contributor.authorMcdermott, MRen_US
dc.contributor.authorPrevec, Len_US
dc.date.accessioned2012-08-08T08:48:30Z-
dc.date.available2012-08-08T08:48:30Z-
dc.date.issued1993en_US
dc.identifier.citationVaccine, 1993, v. 11 n. 12, p. 1191-1197en_US
dc.identifier.issn0264-410Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/157261-
dc.description.abstractThe antigenic and immunogenic potential was examined of human adenovirus type 5 (Ad) recombinants carrying and expressing from one to four tandem repeats of a linear neutralizing epitope from the gD protein of herpes simplex virus type 1 (HSV-1) as a fusion with the β-galactosidase protein. The fusion proteins produced by these Ad vectors in infected cell culture reacted with a herpes simplex virus (HSV) epitope-specific monoclonal antibody to a degree dependent on the number of epitope repeats in the protein. Mice immunized by intraperitoneal injection of the Ad vectors developed an anti-HSV immune response as measured by ELISA and by HSV-1 neutralization assays. The mean antibody titre induced by a single injection of the Ad vector increased with the number of epitope repeats expressed by the recombinant. Any animal that had developed a serum-neutralizing titre of at least 1:80 survived challenge with a normally lethal dose of HSV-2 administered by the intraperitoneal route. Recombinant vectors expressing four repeats of the HSV epitope were as effective in antibody induction and protection as an adenovirus vector carrying and expressing the entire HSV gD protein. These results suggest that the expression of tandem repeats of appropriate epitopic sequences by adenovirus vectors may provide a safe and effective method of immunizing against HSV infection.en_US
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccineen_US
dc.relation.ispartofVaccineen_US
dc.subject.meshAdenoviruses, Human - Genetics - Immunologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshDna - Geneticsen_US
dc.subject.meshEpitopes - Genetics - Immunologyen_US
dc.subject.meshGene Expression - Geneticsen_US
dc.subject.meshGenetic Vectors - Geneticsen_US
dc.subject.meshHela Cellsen_US
dc.subject.meshHerpes Genitalis - Prevention & Controlen_US
dc.subject.meshHerpesvirus 2, Human - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPeptide Fragments - Immunologyen_US
dc.subject.meshRepetitive Sequences, Nucleic Aciden_US
dc.subject.meshVaccines, Synthetic - Pharmacologyen_US
dc.subject.meshVero Cellsen_US
dc.subject.meshViral Envelope Proteins - Genetics - Immunologyen_US
dc.subject.meshViral Vaccines - Genetics - Pharmacologyen_US
dc.subject.meshBeta-Galactosidase - Geneticsen_US
dc.titleImmunogenicity in mice of tandem repeats of an epitope from herpes simplex gD protein when expressed by recombinant adenovirus vectorsen_US
dc.typeArticleen_US
dc.identifier.emailZheng, B:bzheng@hkucc.hku.hken_US
dc.identifier.authorityZheng, B=rp00353en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0264-410X(93)90042-Ven_US
dc.identifier.pmid7504857-
dc.identifier.scopuseid_2-s2.0-0027453680en_US
dc.identifier.volume11en_US
dc.identifier.issue12en_US
dc.identifier.spage1191en_US
dc.identifier.epage1197en_US
dc.identifier.isiWOS:A1993LZ90800004-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridZheng, B=7201780588en_US
dc.identifier.scopusauthoridGraham, FL=35821642700en_US
dc.identifier.scopusauthoridJohnson, DC=35518096100en_US
dc.identifier.scopusauthoridHanke, T=35415389000en_US
dc.identifier.scopusauthoridMcDermott, MR=7202058244en_US
dc.identifier.scopusauthoridPrevec, L=7003455531en_US

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