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Article: Transcription of BamHI-A region of the EBV genome in NPC tissues and B cells

TitleTranscription of BamHI-A region of the EBV genome in NPC tissues and B cells
Authors
Issue Date1992
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
Citation
Virology, 1992, v. 191 n. 1, p. 193-201 How to Cite?
AbstractAnalysis by Northern blotting and sequencing of cDNA clones from a transcription library of a tumor biopsy from a nasopharyngeal carcinoma (NPC) patient showed that the BamHI-A region of the Epstein-Barr virus genome is abundantly and regularly transcribed in tumor tissues from NPC patients. The transcription occurred in a rightward direction terminating between coordinates 160,965 and 160,995, where two polyadenylation sites are located. Rightward transcription of this region also occurred in B lymphoid cells harboring the viral genome, albeit at a lower level than in the tumor tissues. Differential splicing yields a family of related transcripts displaying at least four splicing patterns. Different promoters may be utilized, further contributing to the diversity of this family of transcripts. A 2.8-kb unspliced transcript present in B95-8 cells was probably initiated from a TATA box located in position 158,204, while the other transcripts may utilize other promoters localized to other regions. All the transcripts encompass a putative open reading frame, BARFO, which is predicted to encode a basic protein of about 20 kDa. It shares 40% colinear amino acid sequence homology with the DNA binding region of a transcription factor, ICP4, specified by herpes simplex virus.
Persistent Identifierhttp://hdl.handle.net/10722/157256
ISSN
2015 Impact Factor: 3.2
2015 SCImago Journal Rankings: 1.805
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, HLen_US
dc.contributor.authorLung, MMLen_US
dc.contributor.authorSham, JSTen_US
dc.contributor.authorChoy, DTKen_US
dc.contributor.authorGriffin, BEen_US
dc.contributor.authorNg, MHen_US
dc.date.accessioned2012-08-08T08:48:27Z-
dc.date.available2012-08-08T08:48:27Z-
dc.date.issued1992en_US
dc.identifier.citationVirology, 1992, v. 191 n. 1, p. 193-201en_US
dc.identifier.issn0042-6822en_US
dc.identifier.urihttp://hdl.handle.net/10722/157256-
dc.description.abstractAnalysis by Northern blotting and sequencing of cDNA clones from a transcription library of a tumor biopsy from a nasopharyngeal carcinoma (NPC) patient showed that the BamHI-A region of the Epstein-Barr virus genome is abundantly and regularly transcribed in tumor tissues from NPC patients. The transcription occurred in a rightward direction terminating between coordinates 160,965 and 160,995, where two polyadenylation sites are located. Rightward transcription of this region also occurred in B lymphoid cells harboring the viral genome, albeit at a lower level than in the tumor tissues. Differential splicing yields a family of related transcripts displaying at least four splicing patterns. Different promoters may be utilized, further contributing to the diversity of this family of transcripts. A 2.8-kb unspliced transcript present in B95-8 cells was probably initiated from a TATA box located in position 158,204, while the other transcripts may utilize other promoters localized to other regions. All the transcripts encompass a putative open reading frame, BARFO, which is predicted to encode a basic protein of about 20 kDa. It shares 40% colinear amino acid sequence homology with the DNA binding region of a transcription factor, ICP4, specified by herpes simplex virus.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviroen_US
dc.relation.ispartofVirologyen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshB-Lymphocytes - Microbiologyen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna, Viralen_US
dc.subject.meshDeoxyribonuclease Bamhi - Metabolismen_US
dc.subject.meshGenome, Viralen_US
dc.subject.meshHerpesvirus 4, Human - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshImmediate-Early Proteinsen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Nudeen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNasopharyngeal Neoplasms - Microbiologyen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshRna Splicingen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.subject.meshViral Regulatory And Accessory Proteins - Chemistryen_US
dc.titleTranscription of BamHI-A region of the EBV genome in NPC tissues and B cellsen_US
dc.typeArticleen_US
dc.identifier.emailChen, HL:hlchen@hkucc.hku.hken_US
dc.identifier.authorityChen, HL=rp00383en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0042-6822(92)90181-Nen_US
dc.identifier.pmid1329317-
dc.identifier.scopuseid_2-s2.0-0026802153en_US
dc.identifier.volume191en_US
dc.identifier.issue1en_US
dc.identifier.spage193en_US
dc.identifier.epage201en_US
dc.identifier.isiWOS:A1992JT44600022-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChen, HL=26643315400en_US
dc.identifier.scopusauthoridLung, MML=7006411776en_US
dc.identifier.scopusauthoridSham, JST=7101655565en_US
dc.identifier.scopusauthoridChoy, DTK=7102939127en_US
dc.identifier.scopusauthoridGriffin, BE=7201402684en_US
dc.identifier.scopusauthoridNg, MH=7202076421en_US

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