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Article: Cloning of a functional Burkitt's lymphoma polypeptide-binding protein/78 kDa glucose-regulated protein (BiP/GRP78) gene promoter by the polymerase chain reaction, and its interaction with inducible cellular factors

TitleCloning of a functional Burkitt's lymphoma polypeptide-binding protein/78 kDa glucose-regulated protein (BiP/GRP78) gene promoter by the polymerase chain reaction, and its interaction with inducible cellular factors
Authors
Issue Date1992
PublisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.org
Citation
Biochemical Journal, 1992, v. 286 n. 2, p. 555-559 How to Cite?
AbstractThe promoter of the human gene encoding the stress-responsive protein polypeptide-binding protein/78 kDa glucose-regulated protein (BiP/GRP78) was isolated from Burkitt's lymphoma cells by PCR. This promoter DNA segment (termed BiP670) or one of its 5' deletion derivatives was fused to the bacterial chloramphenicol acetyltransferase gene and introduced into HeLa cells for transient expression. BiP670 retained transcriptional activity at both the basal and Ca2+ ionophore A23187-inducible levels. However, there was no significant increase in promoter activity following a 5h induction with 7 μM-A23187, and less than 5-fold induction at 15h. In contrast, the steady-state mRNA level was induced by 18-fold at 5h. The in vivo transactivation assays with BiP670 5' deletion derivatives indicate that the putative A23187-inducible element is located within a 70 bp DNA segment (i.e. spanning -39 to -107 bp upstream of the transcriptional initiation site). Using an in vitro gel mobility shift assay, A23187-inducible nuclear factors were identified from HeLa cell extracts. DNA-binding competition experiments also suggest that the 70 bp DNA segment contains a potential sequence motif for the binding of the A23187-inducible nuclear factors.
Persistent Identifierhttp://hdl.handle.net/10722/157255
ISSN
2015 Impact Factor: 3.562
2015 SCImago Journal Rankings: 2.582
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChao, CCKen_US
dc.contributor.authorYam, WCen_US
dc.contributor.authorChen, LKen_US
dc.contributor.authorLinChao, Sen_US
dc.date.accessioned2012-08-08T08:48:27Z-
dc.date.available2012-08-08T08:48:27Z-
dc.date.issued1992en_US
dc.identifier.citationBiochemical Journal, 1992, v. 286 n. 2, p. 555-559en_US
dc.identifier.issn0264-6021en_US
dc.identifier.urihttp://hdl.handle.net/10722/157255-
dc.description.abstractThe promoter of the human gene encoding the stress-responsive protein polypeptide-binding protein/78 kDa glucose-regulated protein (BiP/GRP78) was isolated from Burkitt's lymphoma cells by PCR. This promoter DNA segment (termed BiP670) or one of its 5' deletion derivatives was fused to the bacterial chloramphenicol acetyltransferase gene and introduced into HeLa cells for transient expression. BiP670 retained transcriptional activity at both the basal and Ca2+ ionophore A23187-inducible levels. However, there was no significant increase in promoter activity following a 5h induction with 7 μM-A23187, and less than 5-fold induction at 15h. In contrast, the steady-state mRNA level was induced by 18-fold at 5h. The in vivo transactivation assays with BiP670 5' deletion derivatives indicate that the putative A23187-inducible element is located within a 70 bp DNA segment (i.e. spanning -39 to -107 bp upstream of the transcriptional initiation site). Using an in vitro gel mobility shift assay, A23187-inducible nuclear factors were identified from HeLa cell extracts. DNA-binding competition experiments also suggest that the 70 bp DNA segment contains a potential sequence motif for the binding of the A23187-inducible nuclear factors.en_US
dc.languageengen_US
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.orgen_US
dc.relation.ispartofBiochemical Journalen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBinding, Competitiveen_US
dc.subject.meshBurkitt Lymphomaen_US
dc.subject.meshCalcimycin - Pharmacologyen_US
dc.subject.meshCarrier Proteins - Genetics - Metabolismen_US
dc.subject.meshChloramphenicol O-Acetyltransferase - Geneticsen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna - Metabolismen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshHela Cellsen_US
dc.subject.meshHeat-Shock Proteinsen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Chaperonesen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOligonucleotidesen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshRna - Genetics - Isolation & Purificationen_US
dc.subject.meshTranscriptional Activationen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleCloning of a functional Burkitt's lymphoma polypeptide-binding protein/78 kDa glucose-regulated protein (BiP/GRP78) gene promoter by the polymerase chain reaction, and its interaction with inducible cellular factorsen_US
dc.typeArticleen_US
dc.identifier.emailYam, WC:wcyam@hkucc.hku.hken_US
dc.identifier.authorityYam, WC=rp00313en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1382410-
dc.identifier.scopuseid_2-s2.0-0026800725en_US
dc.identifier.volume286en_US
dc.identifier.issue2en_US
dc.identifier.spage555en_US
dc.identifier.epage559en_US
dc.identifier.isiWOS:A1992JM26200035-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridChao, CCK=7403320221en_US
dc.identifier.scopusauthoridYam, WC=7004281720en_US
dc.identifier.scopusauthoridChen, LK=7409442240en_US
dc.identifier.scopusauthoridLinChao, S=7003293256en_US

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