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Article: Rotavirus infection of the oropharynx and respiratory tract in young children

TitleRotavirus infection of the oropharynx and respiratory tract in young children
Authors
Issue Date1991
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763
Citation
Journal Of Medical Virology, 1991, v. 34 n. 1, p. 29-37 How to Cite?
AbstractOropharyngeal aspirates wer obtained from 89 infants hospitalized with respiratory illnesses accompanied or not by diarrhea and 33 control patients without the diseases. Rotavirus was detected from 25 of these patients by immunocytology, isolation of the virus in cultures of MA104 cells, or both. None of the control patients gave a positive result. The infection involves squamoud cells and globet cells probably originating from the oropharynx, and ciliated columnar epithelial cells from the respiratory tract. The virus from 2 specimens was propagated by repeatedly passaging in the cultures and found to have characteristic morphology of rotavirus. The electrophoretic patterns of the viral RNA extracted from them are closely similar to those obtained with the rotavirus genome extracted from the stool of the same patients. Repeated stool specimens were also obtained, and sera were paired from some of these subjects. All but one of the patients who gave a positive virology for their aspirates also showed a significant rise in the titres of common group A rotavirus antibody, neutralizing antibody against one or more of serotypes of rotavirus, or both. Patients who excreted rotavirus in their stools were younger and had significantly lower titres of rotavirus antibodies in their acute sera, than those who shedded the virus in the oropharynx but did not excrete the virus in repeated stool specimens. The prevalence of rotavirus in the oropharyngeal aspirates from these patients surpassed that of adenovirus, respiratory syncytial virus, influenza virus, and herpes simplex virus combined.
Persistent Identifierhttp://hdl.handle.net/10722/157248
ISSN
2015 Impact Factor: 1.998
2015 SCImago Journal Rankings: 1.015
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorJian Zheng, Ben_US
dc.contributor.authorXu Chang, Ren_US
dc.contributor.authorZhang Ma, Gen_US
dc.contributor.authorMin Xie, Jen_US
dc.contributor.authorLiu, Qen_US
dc.contributor.authorRuo Liang, Xen_US
dc.contributor.authorNg, MHen_US
dc.date.accessioned2012-08-08T08:48:24Z-
dc.date.available2012-08-08T08:48:24Z-
dc.date.issued1991en_US
dc.identifier.citationJournal Of Medical Virology, 1991, v. 34 n. 1, p. 29-37en_US
dc.identifier.issn0146-6615en_US
dc.identifier.urihttp://hdl.handle.net/10722/157248-
dc.description.abstractOropharyngeal aspirates wer obtained from 89 infants hospitalized with respiratory illnesses accompanied or not by diarrhea and 33 control patients without the diseases. Rotavirus was detected from 25 of these patients by immunocytology, isolation of the virus in cultures of MA104 cells, or both. None of the control patients gave a positive result. The infection involves squamoud cells and globet cells probably originating from the oropharynx, and ciliated columnar epithelial cells from the respiratory tract. The virus from 2 specimens was propagated by repeatedly passaging in the cultures and found to have characteristic morphology of rotavirus. The electrophoretic patterns of the viral RNA extracted from them are closely similar to those obtained with the rotavirus genome extracted from the stool of the same patients. Repeated stool specimens were also obtained, and sera were paired from some of these subjects. All but one of the patients who gave a positive virology for their aspirates also showed a significant rise in the titres of common group A rotavirus antibody, neutralizing antibody against one or more of serotypes of rotavirus, or both. Patients who excreted rotavirus in their stools were younger and had significantly lower titres of rotavirus antibodies in their acute sera, than those who shedded the virus in the oropharynx but did not excrete the virus in repeated stool specimens. The prevalence of rotavirus in the oropharyngeal aspirates from these patients surpassed that of adenovirus, respiratory syncytial virus, influenza virus, and herpes simplex virus combined.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763en_US
dc.relation.ispartofJournal of Medical Virologyen_US
dc.subject.meshAntibodies, Viral - Analysisen_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshDiarrhea - Complications - Microbiologyen_US
dc.subject.meshFeces - Microbiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshInfanten_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshOropharynx - Immunology - Microbiologyen_US
dc.subject.meshPharyngeal Diseases - Immunology - Microbiologyen_US
dc.subject.meshRna, Viral - Analysisen_US
dc.subject.meshRespiratory Tract Infections - Complications - Immunology - Microbiology - Pathologyen_US
dc.subject.meshRotavirus - Isolation & Purificationen_US
dc.subject.meshRotavirus Infections - Complications - Microbiology - Pathologyen_US
dc.titleRotavirus infection of the oropharynx and respiratory tract in young childrenen_US
dc.typeArticleen_US
dc.identifier.emailJian Zheng, B:bzheng@hkucc.hku.hken_US
dc.identifier.authorityJian Zheng, B=rp00353en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1653306-
dc.identifier.scopuseid_2-s2.0-0025761537en_US
dc.identifier.volume34en_US
dc.identifier.issue1en_US
dc.identifier.spage29en_US
dc.identifier.epage37en_US
dc.identifier.isiWOS:A1991FN18000005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridJian Zheng, B=7201780588en_US
dc.identifier.scopusauthoridXu Chang, R=6503978771en_US
dc.identifier.scopusauthoridZhang Ma, G=6504410848en_US
dc.identifier.scopusauthoridMin Xie, J=6505625173en_US
dc.identifier.scopusauthoridLiu, Q=37099957800en_US
dc.identifier.scopusauthoridRuo Liang, X=6504072114en_US
dc.identifier.scopusauthoridNg, MH=7202076421en_US

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