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Article: Over-expression of AtPAP2 in Camelina sativa leads to faster plant growth and higher seed yield

TitleOver-expression of AtPAP2 in Camelina sativa leads to faster plant growth and higher seed yield
Authors
KeywordsAtpap2
Camelina Sativa
Fatty Acid
Photosynthesis
Sps
Sucrose
Issue Date2012
Citation
Biotechnology for Biofuels, 2012, v. 5, p. 19-28 How to Cite?
AbstractBackground: Lipids extracted from seeds of Camelina sativa have been successfully used as a reliable source of aviation biofuels. This biofuel is environmentally friendly because the drought resistance, frost tolerance and low fertilizer requirement of Camelina sativa allow it to grow on marginal lands. Improving the species growth and seed yield by genetic engineering is therefore a target for the biofuels industry. In Arabidopsis, overexpression of purple acid phosphatase 2 encoded by Arabidopsis (AtPAP2) promotes plant growth by modulating carbon metabolism. Overexpression lines bolt earlier and produce 50% more seeds per plant than wild type. In this study, we explored the effects of overexpressing AtPAP2 in Camelina sativa. Results: Under controlled environmental conditions, overexpression of AtPAP2 in Camelina sativa resulted in longer hypocotyls, earlier flowering, faster growth rate, higher photosynthetic rate and stomatal conductance, increased seed yield and seed size in comparison with the wild-type line and null-lines. Similar to transgenic Arabidopsis, activity of sucrose phosphate synthase in leaves of transgenic Camelina was also significantly up-regulated. Sucrose produced in photosynthetic tissues supplies the building blocks for cellulose, starch and lipids for growth and fuel for anabolic metabolism. Changes in carbon flow and sink/source activities in transgenic lines may affect floral, architectural, and reproductive traits of plants. Conclusions: Lipids extracted from the seeds of Camelina sativa have been used as a major constituent of aviation biofuels. The improved growth rate and seed yield of transgenic Camelina under controlled environmental conditions have the potential to boost oil yield on an area basis in field conditions and thus make Camelina-based biofuels more environmentally friendly and economically attractive. © 2012 Zhang et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/157184
ISSN
2015 Impact Factor: 6.444
2015 SCImago Journal Rankings: 2.557
ISI Accession Number ID
Funding AgencyGrant Number
Initiatives for the Clean Energy and Environment (ICEE) of the University of Hong Kong
General Research FundHKU772710M
HKSAR, ChinaITS158/09
HKU
Funding Information:

This project was supported by the Initiatives for the Clean Energy and Environment (ICEE) of the University of Hong Kong, the General Research Fund (HKU772710M) and the Innovation and Technology Fund (ITS158/09) of the HKSAR, China. We would like to thank Dr. Alice Pilgeram, (Montana State University) for providing WT Camelina sativa variety Suneson. We are thankful to Dr. Lawrence Ramsden for providing the HPLC apparatus. We would like to thank Prof. Samuel Sun (The Chinese University of Hong Kong) for providing the Licor LI-6400XT Portable Photosynthesis System. YZ and FS wish to thank HKU for postgraduate studentships.

References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Yen_US
dc.contributor.authorYu, Len_US
dc.contributor.authorYung, KFen_US
dc.contributor.authorLeung, DYen_US
dc.contributor.authorSun, Fen_US
dc.contributor.authorLim, BLen_US
dc.date.accessioned2012-08-08T08:45:42Z-
dc.date.available2012-08-08T08:45:42Z-
dc.date.issued2012en_US
dc.identifier.citationBiotechnology for Biofuels, 2012, v. 5, p. 19-28en_US
dc.identifier.issn1754-6834en_US
dc.identifier.urihttp://hdl.handle.net/10722/157184-
dc.description.abstractBackground: Lipids extracted from seeds of Camelina sativa have been successfully used as a reliable source of aviation biofuels. This biofuel is environmentally friendly because the drought resistance, frost tolerance and low fertilizer requirement of Camelina sativa allow it to grow on marginal lands. Improving the species growth and seed yield by genetic engineering is therefore a target for the biofuels industry. In Arabidopsis, overexpression of purple acid phosphatase 2 encoded by Arabidopsis (AtPAP2) promotes plant growth by modulating carbon metabolism. Overexpression lines bolt earlier and produce 50% more seeds per plant than wild type. In this study, we explored the effects of overexpressing AtPAP2 in Camelina sativa. Results: Under controlled environmental conditions, overexpression of AtPAP2 in Camelina sativa resulted in longer hypocotyls, earlier flowering, faster growth rate, higher photosynthetic rate and stomatal conductance, increased seed yield and seed size in comparison with the wild-type line and null-lines. Similar to transgenic Arabidopsis, activity of sucrose phosphate synthase in leaves of transgenic Camelina was also significantly up-regulated. Sucrose produced in photosynthetic tissues supplies the building blocks for cellulose, starch and lipids for growth and fuel for anabolic metabolism. Changes in carbon flow and sink/source activities in transgenic lines may affect floral, architectural, and reproductive traits of plants. Conclusions: Lipids extracted from the seeds of Camelina sativa have been used as a major constituent of aviation biofuels. The improved growth rate and seed yield of transgenic Camelina under controlled environmental conditions have the potential to boost oil yield on an area basis in field conditions and thus make Camelina-based biofuels more environmentally friendly and economically attractive. © 2012 Zhang et al; licensee BioMed Central Ltd.en_US
dc.languageengen_US
dc.relation.ispartofBiotechnology for Biofuelsen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectAtpap2en_US
dc.subjectCamelina Sativaen_US
dc.subjectFatty Aciden_US
dc.subjectPhotosynthesisen_US
dc.subjectSpsen_US
dc.subjectSucroseen_US
dc.titleOver-expression of AtPAP2 in Camelina sativa leads to faster plant growth and higher seed yielden_US
dc.typeArticleen_US
dc.identifier.emailLeung, DY:ycleung@hku.hken_US
dc.identifier.emailLim, BL:bllim@hkucc.hku.hken_US
dc.identifier.emailSun, F: sun2011@hku.hk-
dc.identifier.authorityLeung, DY=rp00149en_US
dc.identifier.authorityLim, BL=rp00744en_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1186/1754-6834-5-19en_US
dc.identifier.scopuseid_2-s2.0-84859142185en_US
dc.identifier.hkuros205298-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84859142185&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume5en_US
dc.identifier.isiWOS:000304468300001-
dc.identifier.scopusauthoridZhang, Y=55153292100en_US
dc.identifier.scopusauthoridYu, L=55155109000en_US
dc.identifier.scopusauthoridYung, KF=54796153300en_US
dc.identifier.scopusauthoridLeung, DY=7203002484en_US
dc.identifier.scopusauthoridSun, F=55155033900en_US
dc.identifier.scopusauthoridLim, BL=7201983917en_US

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