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Article: The effect of basic fffiroblast growth factors ( bfgf ) in tendon healing - An in vitro study

TitleThe effect of basic fffiroblast growth factors ( bfgf ) in tendon healing - An in vitro study
Authors
Issue Date1996
PublisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/
Citation
Faseb Journal, 1996, v. 10 n. 3, p. A666 How to Cite?
AbstractTendon healing is subjected to a number of regulating actors. Fibroblast growth factor, FGF, serving a wide spectrum of bioactivities, is a potential mitogenic and chemotactic factor to enhance wound healing. This study investigates the effect of basic FGF (bFGF) on an in vitro wound closure assay, and the proliferative and chemotactic response of cultured rat patellar tendon fibroblasts. In quiescent confluent cells of patellar tendon tissue culture, a cell-free-zone was generated. The width of the wound was measured at 0, 6, 12, 24 hours post injury, in the presence of 0, 2, 10, 50ng/ml of bFGF Cell proliferative response was measured by BrdU (5-Bromo-2'deoxyuridine) incorporation as the number of cells that are in S-phase per unit area. Chemotactic response was measured as the number of cells migrating along a concentration gradient of bFGF per unit area, in a blind well chamber. bFGF, at an optimal concentration of 2-10ng/ml, gives a statistically significant increase in wound closure rate, as well as cell proliferation. However, bFGF seems not to be a chemotactic agent under these conditions.
Persistent Identifierhttp://hdl.handle.net/10722/156847
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorChan, Ben_US
dc.contributor.authorChan, KMen_US
dc.contributor.authorLee, Ken_US
dc.contributor.authorMaffiilli, Nen_US
dc.contributor.authorWebb, Sen_US
dc.date.accessioned2012-08-08T08:44:14Z-
dc.date.available2012-08-08T08:44:14Z-
dc.date.issued1996en_US
dc.identifier.citationFaseb Journal, 1996, v. 10 n. 3, p. A666en_US
dc.identifier.issn0892-6638en_US
dc.identifier.urihttp://hdl.handle.net/10722/156847-
dc.description.abstractTendon healing is subjected to a number of regulating actors. Fibroblast growth factor, FGF, serving a wide spectrum of bioactivities, is a potential mitogenic and chemotactic factor to enhance wound healing. This study investigates the effect of basic FGF (bFGF) on an in vitro wound closure assay, and the proliferative and chemotactic response of cultured rat patellar tendon fibroblasts. In quiescent confluent cells of patellar tendon tissue culture, a cell-free-zone was generated. The width of the wound was measured at 0, 6, 12, 24 hours post injury, in the presence of 0, 2, 10, 50ng/ml of bFGF Cell proliferative response was measured by BrdU (5-Bromo-2'deoxyuridine) incorporation as the number of cells that are in S-phase per unit area. Chemotactic response was measured as the number of cells migrating along a concentration gradient of bFGF per unit area, in a blind well chamber. bFGF, at an optimal concentration of 2-10ng/ml, gives a statistically significant increase in wound closure rate, as well as cell proliferation. However, bFGF seems not to be a chemotactic agent under these conditions.en_US
dc.languageengen_US
dc.publisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/en_US
dc.relation.ispartofFASEB Journalen_US
dc.titleThe effect of basic fffiroblast growth factors ( bfgf ) in tendon healing - An in vitro studyen_US
dc.typeArticleen_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-33749167252en_US
dc.identifier.volume10en_US
dc.identifier.issue3en_US
dc.identifier.spageA666en_US
dc.publisher.placeUnited Statesen_US

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