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Article: Effect of basic fibroblast growth factor: An in vitro study of tendon healing

TitleEffect of basic fibroblast growth factor: An in vitro study of tendon healing
Authors
Issue Date1997
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.corronline.com/
Citation
Clinical Orthopaedics And Related Research, 1997 n. 342, p. 239-247 How to Cite?
AbstractThe effect of basic fibroblast growth factor on the proliferative and chemotactic response of cultured rat patellar tendon fibroblasts was studied in an in vitro wound closure model. In quiescent confluent fibroblast culture, a uniform cell free zone, or wound, was generated mechanically as an in vitro wound. The width of the cell free zone was measured at 0, 6, 12, and 24 hours after the injury, in the presence of 0, 2, 10, or 50 ng/mL of basic fibroblast growth factor. Basic fibroblast growth factor, at a concentration of 10 ng/mL, significantly accelerated wound closure, resulting in almost complete closure by 24 hours after the injury. Basic fibroblast growth factor, at a concentration of 2 ng/mL, significantly enhanced cell proliferation as estimated by 5-Bromo-2'-deoxyuridine incorporation, but increasing the concentration of the growth factor to 50 ng/mL did not show additional improvement. Thus, the enhancement of wound closure by basic fibroblast growth factor may be caused by the cell proliferative response, rather than by chemotaxis.
Persistent Identifierhttp://hdl.handle.net/10722/156692
ISSN
2015 Impact Factor: 3.127
2015 SCImago Journal Rankings: 2.094
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, BPen_US
dc.contributor.authorChan, KMen_US
dc.contributor.authorMaffulli, Nen_US
dc.contributor.authorWebb, Sen_US
dc.contributor.authorLee, KKHen_US
dc.date.accessioned2012-08-08T08:43:34Z-
dc.date.available2012-08-08T08:43:34Z-
dc.date.issued1997en_US
dc.identifier.citationClinical Orthopaedics And Related Research, 1997 n. 342, p. 239-247en_US
dc.identifier.issn0009-921Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/156692-
dc.description.abstractThe effect of basic fibroblast growth factor on the proliferative and chemotactic response of cultured rat patellar tendon fibroblasts was studied in an in vitro wound closure model. In quiescent confluent fibroblast culture, a uniform cell free zone, or wound, was generated mechanically as an in vitro wound. The width of the cell free zone was measured at 0, 6, 12, and 24 hours after the injury, in the presence of 0, 2, 10, or 50 ng/mL of basic fibroblast growth factor. Basic fibroblast growth factor, at a concentration of 10 ng/mL, significantly accelerated wound closure, resulting in almost complete closure by 24 hours after the injury. Basic fibroblast growth factor, at a concentration of 2 ng/mL, significantly enhanced cell proliferation as estimated by 5-Bromo-2'-deoxyuridine incorporation, but increasing the concentration of the growth factor to 50 ng/mL did not show additional improvement. Thus, the enhancement of wound closure by basic fibroblast growth factor may be caused by the cell proliferative response, rather than by chemotaxis.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.corronline.com/en_US
dc.relation.ispartofClinical Orthopaedics and Related Researchen_US
dc.titleEffect of basic fibroblast growth factor: An in vitro study of tendon healingen_US
dc.typeArticleen_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9308546-
dc.identifier.scopuseid_2-s2.0-0343145724en_US
dc.identifier.issue342en_US
dc.identifier.spage239en_US
dc.identifier.epage247en_US
dc.identifier.isiWOS:A1997XV89000031-
dc.publisher.placeUnited Statesen_US

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