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Article: In vivo chromium-enhanced MRI of the retina

TitleIn vivo chromium-enhanced MRI of the retina
Authors
KeywordsChromium-Enhanced Mri
Manganese-Enhanced Mri
Oxidizable Lipid
Retina
Issue Date2012
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0740-3194/
Citation
Magnetic Resonance In Medicine, 2012, v. 68 n. 4, p. 1202-1210 How to Cite?
AbstractChromium (Cr) has been used histologically to stabilize lipid fractions in the retina and is suggested to enhance oxidizable lipids in brain MRI. This study explored the feasibility, sensitivity, and specificity of in vivo chromium-enhanced MRI of retinal lipids by determining its spatiotemporal profiles and toxic effect after intravitreal Cr(VI) injection to normal adult rats. One day after 3 μL Cr(VI) administration at 1-100 mM, the retina exhibited a dose-dependent increase in T1-weighted hyperintensity until 50 mM. Time-dependently, significant T1-weighted hyperintensity persisted up to 2 weeks after 10 mM Cr(VI) administration. Three-dimensional chromium-enhanced MRI of ex vivo normal eyes at isotropic 50-μm resolution showed at least five alternating bands across retinal layers, with the outermost layer being the brightest. This agreed with histology indicating alternating lipid contents with the highest level in the photoreceptor layer of the outer retina. Although Cr(VI) reduction may induce oxidative stress and depolymerize microtubules, manganese-enhanced MRI after chromium-enhanced MRI showed a dose-dependent effect of Cr toxicity on manganese uptake and axonal transport along the visual pathway. These results potentiated future longitudinal chromium-enhanced MRI studies on retinal lipid metabolism upon further optimization of Cr doses with visual cell viability. Magn Reson Med, 2012. © 2011 Wiley Periodicals, Inc. Copyright © 2011 Wiley Periodicals, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/155716
ISSN
2014 Impact Factor: 3.571
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, KCen_HK
dc.contributor.authorFan, SJen_HK
dc.contributor.authorZhou, IYen_HK
dc.contributor.authorWu, EXen_HK
dc.date.accessioned2012-08-08T08:34:59Z-
dc.date.available2012-08-08T08:34:59Z-
dc.date.issued2012en_HK
dc.identifier.citationMagnetic Resonance In Medicine, 2012, v. 68 n. 4, p. 1202-1210en_HK
dc.identifier.issn0740-3194en_HK
dc.identifier.urihttp://hdl.handle.net/10722/155716-
dc.description.abstractChromium (Cr) has been used histologically to stabilize lipid fractions in the retina and is suggested to enhance oxidizable lipids in brain MRI. This study explored the feasibility, sensitivity, and specificity of in vivo chromium-enhanced MRI of retinal lipids by determining its spatiotemporal profiles and toxic effect after intravitreal Cr(VI) injection to normal adult rats. One day after 3 μL Cr(VI) administration at 1-100 mM, the retina exhibited a dose-dependent increase in T1-weighted hyperintensity until 50 mM. Time-dependently, significant T1-weighted hyperintensity persisted up to 2 weeks after 10 mM Cr(VI) administration. Three-dimensional chromium-enhanced MRI of ex vivo normal eyes at isotropic 50-μm resolution showed at least five alternating bands across retinal layers, with the outermost layer being the brightest. This agreed with histology indicating alternating lipid contents with the highest level in the photoreceptor layer of the outer retina. Although Cr(VI) reduction may induce oxidative stress and depolymerize microtubules, manganese-enhanced MRI after chromium-enhanced MRI showed a dose-dependent effect of Cr toxicity on manganese uptake and axonal transport along the visual pathway. These results potentiated future longitudinal chromium-enhanced MRI studies on retinal lipid metabolism upon further optimization of Cr doses with visual cell viability. Magn Reson Med, 2012. © 2011 Wiley Periodicals, Inc. Copyright © 2011 Wiley Periodicals, Inc.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0740-3194/en_HK
dc.relation.ispartofMagnetic Resonance in Medicineen_HK
dc.subjectChromium-Enhanced Mrien_US
dc.subjectManganese-Enhanced Mrien_US
dc.subjectOxidizable Lipiden_US
dc.subjectRetinaen_US
dc.subject.meshAnimalsen_HK
dc.subject.meshChromium - adverse effects - diagnostic use - pharmacokineticsen_HK
dc.subject.meshContrast Media - adverse effectsen_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshImage Enhancement - methodsen_HK
dc.subject.meshLipid Metabolism - physiologyen_HK
dc.subject.meshMagnetic Resonance Imaging - methodsen_HK
dc.subject.meshMolecular Imaging - methodsen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshReproducibility of Resultsen_HK
dc.subject.meshRetina - anatomy & histology - drug effects - metabolismen_HK
dc.subject.meshRetinoscopy - methodsen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.titleIn vivo chromium-enhanced MRI of the retinaen_HK
dc.typeArticleen_HK
dc.identifier.emailZhou, IY: izhou@hku.hken_HK
dc.identifier.emailWu, EX: ewu1@hkucc.hku.hken_HK
dc.identifier.authorityZhou, IY=rp01739en_HK
dc.identifier.authorityWu, EX=rp00193en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/mrm.24123en_HK
dc.identifier.pmid22213133en_HK
dc.identifier.scopuseid_2-s2.0-84866732347en_HK
dc.identifier.hkuros225429-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84866732347&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume68en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1202en_HK
dc.identifier.epage1210en_HK
dc.identifier.isiWOS:000309203500021-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, KC=34968940300en_HK
dc.identifier.scopusauthoridFan, SJ=36514618100en_HK
dc.identifier.scopusauthoridZhou, IY=35424838500en_HK
dc.identifier.scopusauthoridWu, EX=7202128034en_HK

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