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Article: In vivo chromium-enhanced MRI of the retina
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TitleIn vivo chromium-enhanced MRI of the retina
 
AuthorsChan, KC1 2
Fan, SJ1
Zhou, IY1
Wu, EX1
 
KeywordsChromium-Enhanced Mri
Manganese-Enhanced Mri
Oxidizable Lipid
Retina
 
Issue Date2012
 
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0740-3194/
 
CitationMagnetic Resonance In Medicine, 2012, v. 68 n. 4, p. 1202-1210 [How to Cite?]
DOI: http://dx.doi.org/10.1002/mrm.24123
 
AbstractChromium (Cr) has been used histologically to stabilize lipid fractions in the retina and is suggested to enhance oxidizable lipids in brain MRI. This study explored the feasibility, sensitivity, and specificity of in vivo chromium-enhanced MRI of retinal lipids by determining its spatiotemporal profiles and toxic effect after intravitreal Cr(VI) injection to normal adult rats. One day after 3 μL Cr(VI) administration at 1-100 mM, the retina exhibited a dose-dependent increase in T1-weighted hyperintensity until 50 mM. Time-dependently, significant T1-weighted hyperintensity persisted up to 2 weeks after 10 mM Cr(VI) administration. Three-dimensional chromium-enhanced MRI of ex vivo normal eyes at isotropic 50-μm resolution showed at least five alternating bands across retinal layers, with the outermost layer being the brightest. This agreed with histology indicating alternating lipid contents with the highest level in the photoreceptor layer of the outer retina. Although Cr(VI) reduction may induce oxidative stress and depolymerize microtubules, manganese-enhanced MRI after chromium-enhanced MRI showed a dose-dependent effect of Cr toxicity on manganese uptake and axonal transport along the visual pathway. These results potentiated future longitudinal chromium-enhanced MRI studies on retinal lipid metabolism upon further optimization of Cr doses with visual cell viability. Magn Reson Med, 2012. © 2011 Wiley Periodicals, Inc. Copyright © 2011 Wiley Periodicals, Inc.
 
ISSN0740-3194
2013 Impact Factor: 3.398
 
DOIhttp://dx.doi.org/10.1002/mrm.24123
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChan, KC
 
dc.contributor.authorFan, SJ
 
dc.contributor.authorZhou, IY
 
dc.contributor.authorWu, EX
 
dc.date.accessioned2012-08-08T08:34:59Z
 
dc.date.available2012-08-08T08:34:59Z
 
dc.date.issued2012
 
dc.description.abstractChromium (Cr) has been used histologically to stabilize lipid fractions in the retina and is suggested to enhance oxidizable lipids in brain MRI. This study explored the feasibility, sensitivity, and specificity of in vivo chromium-enhanced MRI of retinal lipids by determining its spatiotemporal profiles and toxic effect after intravitreal Cr(VI) injection to normal adult rats. One day after 3 μL Cr(VI) administration at 1-100 mM, the retina exhibited a dose-dependent increase in T1-weighted hyperintensity until 50 mM. Time-dependently, significant T1-weighted hyperintensity persisted up to 2 weeks after 10 mM Cr(VI) administration. Three-dimensional chromium-enhanced MRI of ex vivo normal eyes at isotropic 50-μm resolution showed at least five alternating bands across retinal layers, with the outermost layer being the brightest. This agreed with histology indicating alternating lipid contents with the highest level in the photoreceptor layer of the outer retina. Although Cr(VI) reduction may induce oxidative stress and depolymerize microtubules, manganese-enhanced MRI after chromium-enhanced MRI showed a dose-dependent effect of Cr toxicity on manganese uptake and axonal transport along the visual pathway. These results potentiated future longitudinal chromium-enhanced MRI studies on retinal lipid metabolism upon further optimization of Cr doses with visual cell viability. Magn Reson Med, 2012. © 2011 Wiley Periodicals, Inc. Copyright © 2011 Wiley Periodicals, Inc.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationMagnetic Resonance In Medicine, 2012, v. 68 n. 4, p. 1202-1210 [How to Cite?]
DOI: http://dx.doi.org/10.1002/mrm.24123
 
dc.identifier.doihttp://dx.doi.org/10.1002/mrm.24123
 
dc.identifier.epage1210
 
dc.identifier.hkuros225429
 
dc.identifier.issn0740-3194
2013 Impact Factor: 3.398
 
dc.identifier.issue4
 
dc.identifier.pmid22213133
 
dc.identifier.scopuseid_2-s2.0-84866732347
 
dc.identifier.spage1202
 
dc.identifier.urihttp://hdl.handle.net/10722/155716
 
dc.identifier.volume68
 
dc.languageeng
 
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0740-3194/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofMagnetic Resonance in Medicine
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshChromium - adverse effects - diagnostic use - pharmacokinetics
 
dc.subject.meshContrast Media - adverse effects
 
dc.subject.meshDose-Response Relationship, Drug
 
dc.subject.meshImage Enhancement - methods
 
dc.subject.meshLipid Metabolism - physiology
 
dc.subject.meshMagnetic Resonance Imaging - methods
 
dc.subject.meshMolecular Imaging - methods
 
dc.subject.meshRats
 
dc.subject.meshRats, Sprague-Dawley
 
dc.subject.meshReproducibility of Results
 
dc.subject.meshRetina - anatomy & histology - drug effects - metabolism
 
dc.subject.meshRetinoscopy - methods
 
dc.subject.meshSensitivity and Specificity
 
dc.subjectChromium-Enhanced Mri
 
dc.subjectManganese-Enhanced Mri
 
dc.subjectOxidizable Lipid
 
dc.subjectRetina
 
dc.titleIn vivo chromium-enhanced MRI of the retina
 
dc.typeArticle
 
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<description.abstract>Chromium (Cr) has been used histologically to stabilize lipid fractions in the retina and is suggested to enhance oxidizable lipids in brain MRI. This study explored the feasibility, sensitivity, and specificity of in vivo chromium-enhanced MRI of retinal lipids by determining its spatiotemporal profiles and toxic effect after intravitreal Cr(VI) injection to normal adult rats. One day after 3 &#956;L Cr(VI) administration at 1-100 mM, the retina exhibited a dose-dependent increase in T1-weighted hyperintensity until 50 mM. Time-dependently, significant T1-weighted hyperintensity persisted up to 2 weeks after 10 mM Cr(VI) administration. Three-dimensional chromium-enhanced MRI of ex vivo normal eyes at isotropic 50-&#956;m resolution showed at least five alternating bands across retinal layers, with the outermost layer being the brightest. This agreed with histology indicating alternating lipid contents with the highest level in the photoreceptor layer of the outer retina. Although Cr(VI) reduction may induce oxidative stress and depolymerize microtubules, manganese-enhanced MRI after chromium-enhanced MRI showed a dose-dependent effect of Cr toxicity on manganese uptake and axonal transport along the visual pathway. These results potentiated future longitudinal chromium-enhanced MRI studies on retinal lipid metabolism upon further optimization of Cr doses with visual cell viability. Magn Reson Med, 2012. &#169; 2011 Wiley Periodicals, Inc. Copyright &#169; 2011 Wiley Periodicals, Inc.</description.abstract>
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Author Affiliations
  1. The University of Hong Kong
  2. University of Pittsburgh