Article: In vivo chromium-enhanced MRI of the retina

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TitleIn vivo chromium-enhanced MRI of the retina
AuthorsChan, KC
Fan, SJ
Zhou, IY
Wu, EX
KeywordsChromium-Enhanced Mri
Manganese-Enhanced Mri
Oxidizable Lipid
Retina
Issue Date2012
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0740-3194/
CitationMagnetic Resonance In Medicine, 2012, v. 68 n. 4, p. 1202-1210 [How to Cite?]
DOI: http://dx.doi.org/10.1002/mrm.24123
AbstractChromium (Cr) has been used histologically to stabilize lipid fractions in the retina and is suggested to enhance oxidizable lipids in brain MRI. This study explored the feasibility, sensitivity, and specificity of in vivo chromium-enhanced MRI of retinal lipids by determining its spatiotemporal profiles and toxic effect after intravitreal Cr(VI) injection to normal adult rats. One day after 3 μL Cr(VI) administration at 1-100 mM, the retina exhibited a dose-dependent increase in T1-weighted hyperintensity until 50 mM. Time-dependently, significant T1-weighted hyperintensity persisted up to 2 weeks after 10 mM Cr(VI) administration. Three-dimensional chromium-enhanced MRI of ex vivo normal eyes at isotropic 50-μm resolution showed at least five alternating bands across retinal layers, with the outermost layer being the brightest. This agreed with histology indicating alternating lipid contents with the highest level in the photoreceptor layer of the outer retina. Although Cr(VI) reduction may induce oxidative stress and depolymerize microtubules, manganese-enhanced MRI after chromium-enhanced MRI showed a dose-dependent effect of Cr toxicity on manganese uptake and axonal transport along the visual pathway. These results potentiated future longitudinal chromium-enhanced MRI studies on retinal lipid metabolism upon further optimization of Cr doses with visual cell viability. Magn Reson Med, 2012. © 2011 Wiley Periodicals, Inc. Copyright © 2011 Wiley Periodicals, Inc.
ISSN0740-3194
2011 Impact Factor: 2.964
2011 SCImago Journal Rankings: 0.279
DOIhttp://dx.doi.org/10.1002/mrm.24123
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorChan, KC
dc.contributor.authorFan, SJ
dc.contributor.authorZhou, IY
dc.contributor.authorWu, EX
dc.date.accessioned2012-08-08T08:34:59Z
dc.date.available2012-08-08T08:34:59Z
dc.date.issued2012
dc.description.abstractChromium (Cr) has been used histologically to stabilize lipid fractions in the retina and is suggested to enhance oxidizable lipids in brain MRI. This study explored the feasibility, sensitivity, and specificity of in vivo chromium-enhanced MRI of retinal lipids by determining its spatiotemporal profiles and toxic effect after intravitreal Cr(VI) injection to normal adult rats. One day after 3 μL Cr(VI) administration at 1-100 mM, the retina exhibited a dose-dependent increase in T1-weighted hyperintensity until 50 mM. Time-dependently, significant T1-weighted hyperintensity persisted up to 2 weeks after 10 mM Cr(VI) administration. Three-dimensional chromium-enhanced MRI of ex vivo normal eyes at isotropic 50-μm resolution showed at least five alternating bands across retinal layers, with the outermost layer being the brightest. This agreed with histology indicating alternating lipid contents with the highest level in the photoreceptor layer of the outer retina. Although Cr(VI) reduction may induce oxidative stress and depolymerize microtubules, manganese-enhanced MRI after chromium-enhanced MRI showed a dose-dependent effect of Cr toxicity on manganese uptake and axonal transport along the visual pathway. These results potentiated future longitudinal chromium-enhanced MRI studies on retinal lipid metabolism upon further optimization of Cr doses with visual cell viability. Magn Reson Med, 2012. © 2011 Wiley Periodicals, Inc. Copyright © 2011 Wiley Periodicals, Inc.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationMagnetic Resonance In Medicine, 2012, v. 68 n. 4, p. 1202-1210 [How to Cite?]
DOI: http://dx.doi.org/10.1002/mrm.24123
dc.identifier.doihttp://dx.doi.org/10.1002/mrm.24123
dc.identifier.epage1210
dc.identifier.issn0740-3194
2011 Impact Factor: 2.964
2011 SCImago Journal Rankings: 0.279
dc.identifier.issue4
dc.identifier.pmid22213133
dc.identifier.scopuseid_2-s2.0-84866732347
dc.identifier.spage1202
dc.identifier.urihttp://hdl.handle.net/10722/155716
dc.identifier.volume68
dc.languageeng
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0740-3194/
dc.publisher.placeUnited States
dc.relation.ispartofMagnetic Resonance in Medicine
dc.relation.referencesReferences in Scopus
dc.subject.meshAnimals
dc.subject.meshChromium - adverse effects - diagnostic use - pharmacokinetics
dc.subject.meshContrast Media - adverse effects
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshImage Enhancement - methods
dc.subject.meshLipid Metabolism - physiology
dc.subject.meshMagnetic Resonance Imaging - methods
dc.subject.meshMolecular Imaging - methods
dc.subject.meshRats
dc.subject.meshRats, Sprague-Dawley
dc.subject.meshReproducibility of Results
dc.subject.meshRetina - anatomy & histology - drug effects - metabolism
dc.subject.meshRetinoscopy - methods
dc.subject.meshSensitivity and Specificity
dc.subjectChromium-Enhanced Mri
dc.subjectManganese-Enhanced Mri
dc.subjectOxidizable Lipid
dc.subjectRetina
dc.titleIn vivo chromium-enhanced MRI of the retina
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. University of Pittsburgh