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Article: Diffusion-weighted magnetic resonance spectroscopy: A novel approach to investigate intramyocellular lipids

TitleDiffusion-weighted magnetic resonance spectroscopy: A novel approach to investigate intramyocellular lipids
Authors
Issue Date2011
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0740-3194/
Citation
Magnetic Resonance In Medicine, 2011, v. 66 n. 4, p. 937-944 How to Cite?
AbstractMotivated by the potential difference between intramyocellular lipid (IMCL) and extramyocellular lipid (EMCL) in their microscopically restricted diffusion environments, proton diffusion properties in IMCL and EMCL were characterized by diffusion-weighted magnetic resonance spectroscopy. Ex vivo experiments were conducted on fresh pig lower hindlimb muscle samples. In vivo experiments were performed on the lower hindlimbs of normal adult Sprague-Dawley rats. Ex vivo apparent diffusion coefficients at 20°C were found to be in the ranges of (10.8 ± 0.5) × 10 -6 to (15.8 ± 0.8) × 10 -6 mm 2/s for EMCL and (1.6 ± 0.6) × 10 -6 to (2.4 ± 0.7) × 10 -6 mm 2/s for IMCL depending on the diffusion weighting direction relative to muscle fiber orientation. In vivo apparent diffusion coefficients of EMCL and IMCL were measured as (13.8 ± 0.9) × 10 -6 and (4.6 ± 0.7) × 10 -6 mm 2/s, respectively, along the direction perpendicular to muscle fiber orientation. Our results demonstrated that the apparent diffusion coefficient of EMCL was substantially higher than that of IMCL. Therefore, EMCL could be effectively suppressed by proper diffusion weighting to achieve reliable detection of IMCL despite unknown or/and multiple muscle fiber orientations. Such lipid proton diffusion approach can be used for robust IMCL separation and identification. Furthermore, it may provide new biophysical insights in the investigation of lipid metabolism in obesity and diabetes. © 2011 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/155658
ISSN
2015 Impact Factor: 3.782
2015 SCImago Journal Rankings: 2.197
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grant CouncilHKU7826/10M
Funding Information:

Grant sponsor: Hong Kong Research Grant Council; Grant number: HKU7826/10M.

References

 

DC FieldValueLanguage
dc.contributor.authorXiao, Len_US
dc.contributor.authorWu, EXen_US
dc.date.accessioned2012-08-08T08:34:42Z-
dc.date.available2012-08-08T08:34:42Z-
dc.date.issued2011en_US
dc.identifier.citationMagnetic Resonance In Medicine, 2011, v. 66 n. 4, p. 937-944en_US
dc.identifier.issn0740-3194en_US
dc.identifier.urihttp://hdl.handle.net/10722/155658-
dc.description.abstractMotivated by the potential difference between intramyocellular lipid (IMCL) and extramyocellular lipid (EMCL) in their microscopically restricted diffusion environments, proton diffusion properties in IMCL and EMCL were characterized by diffusion-weighted magnetic resonance spectroscopy. Ex vivo experiments were conducted on fresh pig lower hindlimb muscle samples. In vivo experiments were performed on the lower hindlimbs of normal adult Sprague-Dawley rats. Ex vivo apparent diffusion coefficients at 20°C were found to be in the ranges of (10.8 ± 0.5) × 10 -6 to (15.8 ± 0.8) × 10 -6 mm 2/s for EMCL and (1.6 ± 0.6) × 10 -6 to (2.4 ± 0.7) × 10 -6 mm 2/s for IMCL depending on the diffusion weighting direction relative to muscle fiber orientation. In vivo apparent diffusion coefficients of EMCL and IMCL were measured as (13.8 ± 0.9) × 10 -6 and (4.6 ± 0.7) × 10 -6 mm 2/s, respectively, along the direction perpendicular to muscle fiber orientation. Our results demonstrated that the apparent diffusion coefficient of EMCL was substantially higher than that of IMCL. Therefore, EMCL could be effectively suppressed by proper diffusion weighting to achieve reliable detection of IMCL despite unknown or/and multiple muscle fiber orientations. Such lipid proton diffusion approach can be used for robust IMCL separation and identification. Furthermore, it may provide new biophysical insights in the investigation of lipid metabolism in obesity and diabetes. © 2011 Wiley-Liss, Inc.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0740-3194/en_US
dc.relation.ispartofMagnetic Resonance in Medicineen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDiabetes Mellitus - Metabolismen_US
dc.subject.meshDiffusionen_US
dc.subject.meshExtracellular Space - Physiologyen_US
dc.subject.meshHindlimben_US
dc.subject.meshIntracellular Fluid - Physiologyen_US
dc.subject.meshLipid Metabolismen_US
dc.subject.meshLipids - Analysisen_US
dc.subject.meshMagnetic Resonance Spectroscopy - Methodsen_US
dc.subject.meshMuscle, Skeletal - Metabolismen_US
dc.subject.meshObesity - Metabolismen_US
dc.subject.meshPhantoms, Imagingen_US
dc.subject.meshRatsen_US
dc.subject.meshSwineen_US
dc.titleDiffusion-weighted magnetic resonance spectroscopy: A novel approach to investigate intramyocellular lipidsen_US
dc.typeArticleen_US
dc.identifier.emailWu, EX:ewu1@hkucc.hku.hken_US
dc.identifier.authorityWu, EX=rp00193en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/mrm.23121en_US
dc.identifier.pmid21928357-
dc.identifier.scopuseid_2-s2.0-80053040009en_US
dc.identifier.hkuros206794-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80053040009&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume66en_US
dc.identifier.issue4en_US
dc.identifier.spage937en_US
dc.identifier.epage944en_US
dc.identifier.eissn1522-2594-
dc.identifier.isiWOS:000295356500004-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridXiao, L=53064880500en_US
dc.identifier.scopusauthoridWu, EX=7202128034en_US

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