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Article: Instant magnetic labeling of tumor cells by ultrasound in vitro

TitleInstant magnetic labeling of tumor cells by ultrasound in vitro
Authors
KeywordsIron Oxide Nanoparticle
Magnetic Labeling
Sonoporation
Tumor Cell
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jmmm
Citation
Journal of Magnetism and Magnetic Materials, 2011, v. 323 n. 17, p. 2287-2294 How to Cite?
AbstractMagnetic labeling of living cells creates opportunities for numerous biomedical applications. Here we describe an instantly cell magnetic labeling method based on ultrasound. We present a detailed study on the ultrasound performance of a simple and efficient labeling protocol for H-22 cells in vitro. High frequency focus ultrasound was investigated as an alternative method to achieve instant cell labeling with the magnetic particles without the need for adjunct agents or initiating cell cultures. Mean diameter of 168 nm dextran-T40 coated superparamagnetic iron oxide (SPIO) nanoparticles were prepared by means of classical coprecipitation in solution in our laboratory. H-22 tumor cells suspended in phosphate-buffered saline (PBS, pH=7.2) were exposed to ultrasound at 1.37 MHz for up to 120 s in the presence of SPIOs. The cellular uptake of iron oxide nanoparticles was detected by prussion blue staining. The viability of cells was determined by a trypan blue exclusion test. At 2 W power and 60 s ultrasound exposure in presence of 410 μg/ml SPIOs, H-22 cell labeling efficiency reached 69.4±6.3% and the labeled cells exhibited an iron content of 10.38±2.43 pg per cell. Furthermore, 95.2±3.2% cells remained viable. The results indicated that the ultrasound protocol could be potentially applied to label cells with large-sized magnetic particles. We also calculated the shear stress at the 2 W power and 1.37 MHz used in experiments. The results showed that the shear stress threshold for ultrasonically induced H-22 cell reparable sonoporation was 697 Pa. These findings provide a quantitative guidance in designing ultrasound protocols for cell labeling. Copyright © 2011 Published by Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/155616
ISSN
2015 Impact Factor: 2.357
2015 SCImago Journal Rankings: 0.776
ISI Accession Number ID
Funding AgencyGrant Number
Natural Science Foundation of China10674090
30970797
Natural Science Foundation of Shaanxi Province, China2010JM 1002
National Basic Research Program of China (973 Program)2010CB7332603
Funding Information:

This work was funded by the Natural Science Foundation of China (Grant no. 10674090, 30970797), the Natural Science Foundation of Shaanxi Province, China (grant 2010JM 1002) and National Basic Research Program of China (973 Program, Grant no. 2010CB7332603).

References

 

DC FieldValueLanguage
dc.contributor.authorMo, Ren_US
dc.contributor.authorYang, Jen_US
dc.contributor.authorWu, EXen_US
dc.contributor.authorLin, Sen_US
dc.date.accessioned2012-08-08T08:34:23Z-
dc.date.available2012-08-08T08:34:23Z-
dc.date.issued2011en_US
dc.identifier.citationJournal of Magnetism and Magnetic Materials, 2011, v. 323 n. 17, p. 2287-2294en_US
dc.identifier.issn0304-8853en_US
dc.identifier.urihttp://hdl.handle.net/10722/155616-
dc.description.abstractMagnetic labeling of living cells creates opportunities for numerous biomedical applications. Here we describe an instantly cell magnetic labeling method based on ultrasound. We present a detailed study on the ultrasound performance of a simple and efficient labeling protocol for H-22 cells in vitro. High frequency focus ultrasound was investigated as an alternative method to achieve instant cell labeling with the magnetic particles without the need for adjunct agents or initiating cell cultures. Mean diameter of 168 nm dextran-T40 coated superparamagnetic iron oxide (SPIO) nanoparticles were prepared by means of classical coprecipitation in solution in our laboratory. H-22 tumor cells suspended in phosphate-buffered saline (PBS, pH=7.2) were exposed to ultrasound at 1.37 MHz for up to 120 s in the presence of SPIOs. The cellular uptake of iron oxide nanoparticles was detected by prussion blue staining. The viability of cells was determined by a trypan blue exclusion test. At 2 W power and 60 s ultrasound exposure in presence of 410 μg/ml SPIOs, H-22 cell labeling efficiency reached 69.4±6.3% and the labeled cells exhibited an iron content of 10.38±2.43 pg per cell. Furthermore, 95.2±3.2% cells remained viable. The results indicated that the ultrasound protocol could be potentially applied to label cells with large-sized magnetic particles. We also calculated the shear stress at the 2 W power and 1.37 MHz used in experiments. The results showed that the shear stress threshold for ultrasonically induced H-22 cell reparable sonoporation was 697 Pa. These findings provide a quantitative guidance in designing ultrasound protocols for cell labeling. Copyright © 2011 Published by Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jmmmen_US
dc.relation.ispartofJournal of Magnetism and Magnetic Materialsen_US
dc.subjectIron Oxide Nanoparticleen_US
dc.subjectMagnetic Labelingen_US
dc.subjectSonoporationen_US
dc.subjectTumor Cellen_US
dc.titleInstant magnetic labeling of tumor cells by ultrasound in vitroen_US
dc.typeArticleen_US
dc.identifier.emailWu, EX:ewu1@hkucc.hku.hken_US
dc.identifier.authorityWu, EX=rp00193en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jmmm.2011.04.008en_US
dc.identifier.scopuseid_2-s2.0-79957504631en_US
dc.identifier.hkuros206810-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79957504631&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume323en_US
dc.identifier.issue17en_US
dc.identifier.spage2287en_US
dc.identifier.epage2294en_US
dc.identifier.isiWOS:000290900500010-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridMo, R=26321792100en_US
dc.identifier.scopusauthoridYang, J=37098357500en_US
dc.identifier.scopusauthoridWu, EX=7202128034en_US
dc.identifier.scopusauthoridLin, S=39661151100en_US
dc.identifier.citeulike9227539-

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