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Article: Three-dimensional microscopy and sectional image reconstruction using optical scanning holography

TitleThree-dimensional microscopy and sectional image reconstruction using optical scanning holography
Authors
Issue Date2009
PublisherOptical Society of America. The Journal's web site is located at http://ao.osa.org/journal/ao/about.cfm
Citation
Applied Optics, 2009, v. 48 n. 34, p. H113-H119 How to Cite?
AbstractFast acquisition and high axial resolution are two primary requirements for three-dimensional microscopy. However, they are sometimes conflicting: imaging modalities such as confocal imaging can deliver superior resolution at the expense of sequential acquisition at different axial planes, which is a time-consuming process. Optical scanning holography (OSH) promises to deliver a good trade-off between these two goals. With just a single scan, we can capture the entire three-dimensional volume in a digital hologram; the data can then be processed to obtain the individual sections. An accurate modeling of the imaging system is key to devising an appropriate image reconstruction algorithm, especially for real data where random noise and other imaging imperfections must be taken into account. In this paper we demonstrate sectional image reconstruction by applying an inverse imaging sectioning technique to experimental OSH data of biological specimens and visualizing the sections using the OSA Interactive Science Publishing software. © 2009 Optical Society of America.
Persistent Identifierhttp://hdl.handle.net/10722/155550
ISSN
2010 Impact Factor: 1.707
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLam, EYen_US
dc.contributor.authorZhang, Xen_US
dc.contributor.authorVo, Hen_US
dc.contributor.authorPoon, TCen_US
dc.contributor.authorIndebetouw, Gen_US
dc.date.accessioned2012-08-08T08:34:03Z-
dc.date.available2012-08-08T08:34:03Z-
dc.date.issued2009en_US
dc.identifier.citationApplied Optics, 2009, v. 48 n. 34, p. H113-H119en_US
dc.identifier.issn0003-6935en_US
dc.identifier.urihttp://hdl.handle.net/10722/155550-
dc.description.abstractFast acquisition and high axial resolution are two primary requirements for three-dimensional microscopy. However, they are sometimes conflicting: imaging modalities such as confocal imaging can deliver superior resolution at the expense of sequential acquisition at different axial planes, which is a time-consuming process. Optical scanning holography (OSH) promises to deliver a good trade-off between these two goals. With just a single scan, we can capture the entire three-dimensional volume in a digital hologram; the data can then be processed to obtain the individual sections. An accurate modeling of the imaging system is key to devising an appropriate image reconstruction algorithm, especially for real data where random noise and other imaging imperfections must be taken into account. In this paper we demonstrate sectional image reconstruction by applying an inverse imaging sectioning technique to experimental OSH data of biological specimens and visualizing the sections using the OSA Interactive Science Publishing software. © 2009 Optical Society of America.en_US
dc.languageengen_US
dc.publisherOptical Society of America. The Journal's web site is located at http://ao.osa.org/journal/ao/about.cfmen_US
dc.relation.ispartofApplied Opticsen_US
dc.subject.meshComputer-Aided Designen_US
dc.subject.meshEquipment Designen_US
dc.subject.meshEquipment Failure Analysisen_US
dc.subject.meshHolography - Instrumentationen_US
dc.subject.meshImage Enhancement - Instrumentationen_US
dc.subject.meshImage Interpretation, Computer-Assisted - Instrumentationen_US
dc.subject.meshImaging, Three-Dimensional - Instrumentationen_US
dc.subject.meshMicroscopy, Confocal - Instrumentationen_US
dc.titleThree-dimensional microscopy and sectional image reconstruction using optical scanning holographyen_US
dc.typeArticleen_US
dc.identifier.emailLam, EY:elam@eee.hku.hken_US
dc.identifier.authorityLam, EY=rp00131en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1364/AO.48.00H113en_US
dc.identifier.pmid19956281-
dc.identifier.scopuseid_2-s2.0-73649144216en_US
dc.identifier.hkuros171661-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-73649144216&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume48en_US
dc.identifier.issue34en_US
dc.identifier.spageH113en_US
dc.identifier.epageH119en_US
dc.identifier.eissn2155-3165-
dc.identifier.isiWOS:000272284600016-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLam, EY=7102890004en_US
dc.identifier.scopusauthoridZhang, X=7410271827en_US
dc.identifier.scopusauthoridVo, H=35323357400en_US
dc.identifier.scopusauthoridPoon, TC=7006151719en_US
dc.identifier.scopusauthoridIndebetouw, G=7006641624en_US

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