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Article: Temperature-controlled system of PCR chips

TitleTemperature-controlled system of PCR chips
Authors
KeywordsDNA
PCR chips
Temperature-controlled system
Issue Date2001
Citation
Huazhong Ligong Daxue Xuebao/Journal Huazhong (Central China) University Of Science And Technology, 2001, v. 29 n. 12, p. 45-47 How to Cite?
AbstractA temperature-controlled system of PCR chips for DNA amplification which possesses the characteristics of low cost and high controlling precision is developed by using digital-circuit temperature-controlled technique based on microcontroller, PWM technique and PID temperature-controlled algorithm. The experimental results show that this system can well perform cycling control between different temperatures (37°C, 55°C, 72°C and 94°C) required for PCR chips and its controlling precision is ±0.1°C. The thermoelectric cooler is used as thermal generator and a heat sink (by controlling the polarity of supply power) and rise/fall rates of temperature are 4°C/s and 2.2°C/s respectively.
Persistent Identifierhttp://hdl.handle.net/10722/155155
ISSN

 

DC FieldValueLanguage
dc.contributor.authorXu, Jen_HK
dc.contributor.authorLai, PTen_HK
dc.contributor.authorGan, Xen_HK
dc.contributor.authorZhong, Den_HK
dc.date.accessioned2012-08-08T08:32:06Z-
dc.date.available2012-08-08T08:32:06Z-
dc.date.issued2001en_HK
dc.identifier.citationHuazhong Ligong Daxue Xuebao/Journal Huazhong (Central China) University Of Science And Technology, 2001, v. 29 n. 12, p. 45-47en_HK
dc.identifier.issn1000-8616en_HK
dc.identifier.urihttp://hdl.handle.net/10722/155155-
dc.description.abstractA temperature-controlled system of PCR chips for DNA amplification which possesses the characteristics of low cost and high controlling precision is developed by using digital-circuit temperature-controlled technique based on microcontroller, PWM technique and PID temperature-controlled algorithm. The experimental results show that this system can well perform cycling control between different temperatures (37°C, 55°C, 72°C and 94°C) required for PCR chips and its controlling precision is ±0.1°C. The thermoelectric cooler is used as thermal generator and a heat sink (by controlling the polarity of supply power) and rise/fall rates of temperature are 4°C/s and 2.2°C/s respectively.en_HK
dc.languageengen_US
dc.relation.ispartofHuazhong Ligong Daxue Xuebao/Journal Huazhong (Central China) University of Science and Technologyen_HK
dc.subjectDNAen_HK
dc.subjectPCR chipsen_HK
dc.subjectTemperature-controlled systemen_HK
dc.titleTemperature-controlled system of PCR chipsen_HK
dc.typeArticleen_HK
dc.identifier.emailXu, J: jpxu@eee.hku.hken_HK
dc.identifier.emailLai, PT: laip@eee.hku.hken_HK
dc.identifier.authorityXu, J=rp00197en_HK
dc.identifier.authorityLai, PT=rp00130en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0035706531en_HK
dc.identifier.volume29en_HK
dc.identifier.issue12en_HK
dc.identifier.spage45en_HK
dc.identifier.epage47en_HK
dc.identifier.scopusauthoridXu, J=7407004696en_HK
dc.identifier.scopusauthoridLai, PT=7202946460en_HK
dc.identifier.scopusauthoridGan, X=7103333919en_HK
dc.identifier.scopusauthoridZhong, D=23394223100en_HK

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