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Article: Temperature-controlled system of PCR chips
Title | Temperature-controlled system of PCR chips |
---|---|
Authors | |
Keywords | DNA PCR chips Temperature-controlled system |
Issue Date | 2001 |
Citation | Huazhong Ligong Daxue Xuebao/Journal Huazhong (Central China) University Of Science And Technology, 2001, v. 29 n. 12, p. 45-47 How to Cite? |
Abstract | A temperature-controlled system of PCR chips for DNA amplification which possesses the characteristics of low cost and high controlling precision is developed by using digital-circuit temperature-controlled technique based on microcontroller, PWM technique and PID temperature-controlled algorithm. The experimental results show that this system can well perform cycling control between different temperatures (37°C, 55°C, 72°C and 94°C) required for PCR chips and its controlling precision is ±0.1°C. The thermoelectric cooler is used as thermal generator and a heat sink (by controlling the polarity of supply power) and rise/fall rates of temperature are 4°C/s and 2.2°C/s respectively. |
Persistent Identifier | http://hdl.handle.net/10722/155155 |
ISSN |
DC Field | Value | Language |
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dc.contributor.author | Xu, J | en_HK |
dc.contributor.author | Lai, PT | en_HK |
dc.contributor.author | Gan, X | en_HK |
dc.contributor.author | Zhong, D | en_HK |
dc.date.accessioned | 2012-08-08T08:32:06Z | - |
dc.date.available | 2012-08-08T08:32:06Z | - |
dc.date.issued | 2001 | en_HK |
dc.identifier.citation | Huazhong Ligong Daxue Xuebao/Journal Huazhong (Central China) University Of Science And Technology, 2001, v. 29 n. 12, p. 45-47 | en_HK |
dc.identifier.issn | 1000-8616 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/155155 | - |
dc.description.abstract | A temperature-controlled system of PCR chips for DNA amplification which possesses the characteristics of low cost and high controlling precision is developed by using digital-circuit temperature-controlled technique based on microcontroller, PWM technique and PID temperature-controlled algorithm. The experimental results show that this system can well perform cycling control between different temperatures (37°C, 55°C, 72°C and 94°C) required for PCR chips and its controlling precision is ±0.1°C. The thermoelectric cooler is used as thermal generator and a heat sink (by controlling the polarity of supply power) and rise/fall rates of temperature are 4°C/s and 2.2°C/s respectively. | en_HK |
dc.language | eng | en_US |
dc.relation.ispartof | Huazhong Ligong Daxue Xuebao/Journal Huazhong (Central China) University of Science and Technology | en_HK |
dc.subject | DNA | en_HK |
dc.subject | PCR chips | en_HK |
dc.subject | Temperature-controlled system | en_HK |
dc.title | Temperature-controlled system of PCR chips | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Xu, J: jpxu@eee.hku.hk | en_HK |
dc.identifier.email | Lai, PT: laip@eee.hku.hk | en_HK |
dc.identifier.authority | Xu, J=rp00197 | en_HK |
dc.identifier.authority | Lai, PT=rp00130 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.scopus | eid_2-s2.0-0035706531 | en_HK |
dc.identifier.volume | 29 | en_HK |
dc.identifier.issue | 12 | en_HK |
dc.identifier.spage | 45 | en_HK |
dc.identifier.epage | 47 | en_HK |
dc.identifier.scopusauthorid | Xu, J=7407004696 | en_HK |
dc.identifier.scopusauthorid | Lai, PT=7202946460 | en_HK |
dc.identifier.scopusauthorid | Gan, X=7103333919 | en_HK |
dc.identifier.scopusauthorid | Zhong, D=23394223100 | en_HK |
dc.identifier.issnl | 1000-8616 | - |