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Article: Rapid in vivo monitoring of chemotherapeutic response using weighted sodium magnetic resonance imaging

TitleRapid in vivo monitoring of chemotherapeutic response using weighted sodium magnetic resonance imaging
Authors
Issue Date2000
Citation
Clinical Cancer Research, 2000, v. 6 n. 6, p. 2146-2156 How to Cite?
AbstractA novel pulse sequence strategy uses sodium magnetic resonance imaging to monitor the response to chemotherapy of mouse xenograft tumors propagated from human prostate cancer cell lines. An inversion pulse suppresses sodium with long longitudinal relaxation times, weighting the image toward intracellular sodium nuclei. Comparing these weighted sodium images before and 24 h after administration of antineoplastics, we measured a 36 ± 4% (P < 0.001; n = 16) increase in signal intensity. Experiments with these same drugs and cells, treated in culture, detected a significant intracellular sodium elevation (10-20 mM) using a ratiometric fluorescent dye. Flow cytometry studies showed that this elevation preceded cell death by apoptosis, as determined by fluorescent end-labeling of apoptotic nuclei or Annexin V binding. Histopathology on formalin-fixed sections of explanted tumors confirmed that drug administration reduces proliferation (2.2 versus 8.6 mitotic figures per high power field; P < 0.0001), an effect that inversely correlates with the sodium magnetic resonance image response on a tumor-to-tumor basis (P < 0.02; n = 10). Morphological features, such as central zones of nonviable cells, rims of active apoptosis, and areas of viable tumor, could be distinguished by comparing weighted and unweighted images. Advantages of this sodium imaging technique include rapid determination of drug efficacy, improved diagnosis of lesions, ease of coregistration with high resolution proton magnetic resonance imaging, and absence of costly or toxic reagents.
Persistent Identifierhttp://hdl.handle.net/10722/155127
ISSN
2015 Impact Factor: 8.738
2015 SCImago Journal Rankings: 5.314
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKline, RPen_US
dc.contributor.authorWu, EXen_US
dc.contributor.authorPetrylak, DPen_US
dc.contributor.authorSzabolcs, Men_US
dc.contributor.authorAlderson, POen_US
dc.contributor.authorWeisfeldt, MLen_US
dc.contributor.authorCannon, Pen_US
dc.contributor.authorKatz, Jen_US
dc.date.accessioned2012-08-08T08:31:58Z-
dc.date.available2012-08-08T08:31:58Z-
dc.date.issued2000en_US
dc.identifier.citationClinical Cancer Research, 2000, v. 6 n. 6, p. 2146-2156en_US
dc.identifier.issn1078-0432en_US
dc.identifier.urihttp://hdl.handle.net/10722/155127-
dc.description.abstractA novel pulse sequence strategy uses sodium magnetic resonance imaging to monitor the response to chemotherapy of mouse xenograft tumors propagated from human prostate cancer cell lines. An inversion pulse suppresses sodium with long longitudinal relaxation times, weighting the image toward intracellular sodium nuclei. Comparing these weighted sodium images before and 24 h after administration of antineoplastics, we measured a 36 ± 4% (P < 0.001; n = 16) increase in signal intensity. Experiments with these same drugs and cells, treated in culture, detected a significant intracellular sodium elevation (10-20 mM) using a ratiometric fluorescent dye. Flow cytometry studies showed that this elevation preceded cell death by apoptosis, as determined by fluorescent end-labeling of apoptotic nuclei or Annexin V binding. Histopathology on formalin-fixed sections of explanted tumors confirmed that drug administration reduces proliferation (2.2 versus 8.6 mitotic figures per high power field; P < 0.0001), an effect that inversely correlates with the sodium magnetic resonance image response on a tumor-to-tumor basis (P < 0.02; n = 10). Morphological features, such as central zones of nonviable cells, rims of active apoptosis, and areas of viable tumor, could be distinguished by comparing weighted and unweighted images. Advantages of this sodium imaging technique include rapid determination of drug efficacy, improved diagnosis of lesions, ease of coregistration with high resolution proton magnetic resonance imaging, and absence of costly or toxic reagents.en_US
dc.languageengen_US
dc.relation.ispartofClinical Cancer Researchen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnnexin A5 - Metabolismen_US
dc.subject.meshAntineoplastic Agents, Phytogenic - Pharmacologyen_US
dc.subject.meshApoptosisen_US
dc.subject.meshCell Nucleus - Metabolismen_US
dc.subject.meshEtoposide - Pharmacologyen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshFluorescent Antibody Techniqueen_US
dc.subject.meshFluorescent Dyes - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMagnetic Resonance Imaging - Methodsen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshNeoplasm Transplantation - Pathologyen_US
dc.subject.meshPaclitaxel - Analogs & Derivatives - Pharmacologyen_US
dc.subject.meshPhantoms, Imagingen_US
dc.subject.meshProstatic Neoplasms - Drug Therapy - Pathologyen_US
dc.subject.meshSodiumen_US
dc.subject.meshSodium Chloride - Chemistryen_US
dc.subject.meshTaxoidsen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTreatment Outcomeen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleRapid in vivo monitoring of chemotherapeutic response using weighted sodium magnetic resonance imagingen_US
dc.typeArticleen_US
dc.identifier.emailWu, EX:ewu1@hkucc.hku.hken_US
dc.identifier.authorityWu, EX=rp00193en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid10873063-
dc.identifier.scopuseid_2-s2.0-0034125873en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034125873&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume6en_US
dc.identifier.issue6en_US
dc.identifier.spage2146en_US
dc.identifier.epage2156en_US
dc.identifier.isiWOS:000087540300005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKline, RP=7103055795en_US
dc.identifier.scopusauthoridWu, EX=7202128034en_US
dc.identifier.scopusauthoridPetrylak, DP=7003419136en_US
dc.identifier.scopusauthoridSzabolcs, M=7007059450en_US
dc.identifier.scopusauthoridAlderson, PO=7102760522en_US
dc.identifier.scopusauthoridWeisfeldt, ML=7005272078en_US
dc.identifier.scopusauthoridCannon, P=7102373447en_US
dc.identifier.scopusauthoridKatz, J=7403968068en_US

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