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Article: Biocide resistance of Candida and Escherichia coli biofilms is associated with higher antioxidative capacities

TitleBiocide resistance of Candida and Escherichia coli biofilms is associated with higher antioxidative capacities
Authors
KeywordsBiocides
Biofilm
Candida
Escherichia Coli
Issue Date2012
PublisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jhin
Citation
Journal Of Hospital Infection, 2012, v. 81 n. 2, p. 79-86 How to Cite?
AbstractBackground: Most clinical guidelines for the use of biocides have been developed for planktonic micro-organisms, but in nature, most micro-organisms live as surface-adherent communities or biofilms. Aim: To evaluate the effectiveness of commonly used biocides against . Escherichia coli and . Candida spp. in three distinct growth phases: planktonic, adhesion and biofilm. Methods: Ultrastructural, architectural and cellular viability changes following a 5 min exposure to biocide were monitored by scanning electron microscopy and confocal laser scanning microscopy using fluorescent dyes. Comparative transcript expression of the antioxidants . SOD1 and . CAT1 in the planktonic and biofilm phases was evaluated using quantitative real-time polymerase chain reaction. Findings: E. coli and . Candida spp. in the planktonic phase were susceptible to all the tested biocides at the recommended concentrations. However, early adhesion and late biofilm phases of both were less susceptible to the biocides, and exceeded the recommended concentrations on several occasions. A short period of biocide exposure failed to fully eradicate the adherent microbial cells, and they recovered from the biocide challenge, forming biofilm on the biocide-treated surfaces. The biofilm phase showed higher expression of . SOD1 and . CAT1. Conclusion: The recommended concentrations of biocides for clinical disinfection in the hospital setting may not fully eradicate the adhesion or biofilm phases of . E. coli and . Candida spp. Higher antioxidative capacities in microbial biofilms may be responsible for the resistance of biofilms against clinical biocides. © 2011 The Healthcare Infection Society.
Persistent Identifierhttp://hdl.handle.net/10722/154731
ISSN
2015 Impact Factor: 2.655
2015 SCImago Journal Rankings: 1.349
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants Council, RGCHKU 7624/06M
Funding Information:

This work was supported by the Hong Kong Research Grants Council, RGC No. HKU 7624/06M.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLeung, CYen_US
dc.contributor.authorChan, YCen_US
dc.contributor.authorSamaranayake, LPen_US
dc.contributor.authorSeneviratne, CJen_US
dc.date.accessioned2012-08-08T08:27:10Z-
dc.date.available2012-08-08T08:27:10Z-
dc.date.issued2012en_US
dc.identifier.citationJournal Of Hospital Infection, 2012, v. 81 n. 2, p. 79-86en_US
dc.identifier.issn0195-6701en_US
dc.identifier.urihttp://hdl.handle.net/10722/154731-
dc.description.abstractBackground: Most clinical guidelines for the use of biocides have been developed for planktonic micro-organisms, but in nature, most micro-organisms live as surface-adherent communities or biofilms. Aim: To evaluate the effectiveness of commonly used biocides against . Escherichia coli and . Candida spp. in three distinct growth phases: planktonic, adhesion and biofilm. Methods: Ultrastructural, architectural and cellular viability changes following a 5 min exposure to biocide were monitored by scanning electron microscopy and confocal laser scanning microscopy using fluorescent dyes. Comparative transcript expression of the antioxidants . SOD1 and . CAT1 in the planktonic and biofilm phases was evaluated using quantitative real-time polymerase chain reaction. Findings: E. coli and . Candida spp. in the planktonic phase were susceptible to all the tested biocides at the recommended concentrations. However, early adhesion and late biofilm phases of both were less susceptible to the biocides, and exceeded the recommended concentrations on several occasions. A short period of biocide exposure failed to fully eradicate the adherent microbial cells, and they recovered from the biocide challenge, forming biofilm on the biocide-treated surfaces. The biofilm phase showed higher expression of . SOD1 and . CAT1. Conclusion: The recommended concentrations of biocides for clinical disinfection in the hospital setting may not fully eradicate the adhesion or biofilm phases of . E. coli and . Candida spp. Higher antioxidative capacities in microbial biofilms may be responsible for the resistance of biofilms against clinical biocides. © 2011 The Healthcare Infection Society.en_US
dc.languageengen_US
dc.publisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jhinen_US
dc.relation.ispartofJournal of Hospital Infectionen_US
dc.subjectBiocidesen_US
dc.subjectBiofilmen_US
dc.subjectCandidaen_US
dc.subjectEscherichia Colien_US
dc.titleBiocide resistance of Candida and Escherichia coli biofilms is associated with higher antioxidative capacitiesen_US
dc.typeArticleen_US
dc.identifier.emailSeneviratne, CJ:jaya@hku.hken_US
dc.identifier.authoritySeneviratne, CJ=rp01372en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jhin.2011.09.014en_US
dc.identifier.pmid22595316-
dc.identifier.scopuseid_2-s2.0-84861713812en_US
dc.identifier.hkuros204601-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84861713812&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume81en_US
dc.identifier.issue2en_US
dc.identifier.spage79en_US
dc.identifier.epage86en_US
dc.identifier.eissn1532-2939-
dc.identifier.isiWOS:000304676400003-
dc.publisher.placeUnited Kingdomen_US
dc.relation.projectCandida biofilms: molecular mechanisms and clinical implications-
dc.identifier.scopusauthoridLeung, CY=55215126800en_US
dc.identifier.scopusauthoridChan, YC=55215479000en_US
dc.identifier.scopusauthoridSamaranayake, LP=55164536100en_US
dc.identifier.scopusauthoridSeneviratne, CJ=6701897753en_US

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