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Article: The inhibitory effects of silver diamine fluoride at different concentrations on matrix metalloproteinases

TitleThe inhibitory effects of silver diamine fluoride at different concentrations on matrix metalloproteinases
Authors
KeywordsArrest
Caries
Dentin
Matrix metalloproteinases
Sodium fluoride
Issue Date2012
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/dental
Citation
Dental Materials, 2012, v. 28 n. 8, p. 903-908 How to Cite?
AbstractOBJECTIVE: To study the inhibitory effect of various commercially available concentrations of silver diamine fluoride (SDF) solutions on matrix metalloproteinases (MMPs). METHODS: Three SDF solutions with concentrations at 38%, 30% and 12% were studied. Two sodium fluoride (NaF) solutions at 10% and 3% were prepared, and they had the same fluoride ion concentrations as 38% and 12% SDF, respectively. Two silver nitrate (AgNO(3)) solutions at 42% and 13% were also prepared, and they had the same silver ion concentrations as 38% and 12% SDF, respectively. Ten samples of each experimental solution were used to study their inhibitory effect on three MMPs, which were MMP-2 (gelatinase A), MMP-8 (neutrophil collagenase) and MMP-9 (gelatinase B) using MMP assay kits. Positive control containing assay buffer at pH 9 and MMPs dilution was used to calculate the percentage inhibition. RESULTS: The percentage inhibition of 38%, 30% and 12% SDF on MMP-2 were 79%, 60% and 17%, respectively (p<0.001); on MMP-8 were 94%, 85% and 77%, respectively (p<0.001); on MMP-9 were 82%, 65% and 60%, respectively (p<0.001). The percentage inhibition on MMP-2, MMP-8 and MMP-9 by 38% SDF was significantly higher than the corresponding percentage inhibition by 10% NaF and 42% AgNO(3). SIGNIFICANCE: Greater inhibitory effect on MMPs was found with higher concentration of SDF solution. SDF had more inhibition on MMPs than solutions of NaF and AgNO(3) containing equivalent concentration of fluoride and silver ions, respectively.
Persistent Identifierhttp://hdl.handle.net/10722/154722
ISSN
2015 Impact Factor: 3.931
2015 SCImago Journal Rankings: 1.339
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMei, MLen_US
dc.contributor.authorLi, QLen_US
dc.contributor.authorChu, CHen_US
dc.contributor.authorYiu, CKYen_US
dc.contributor.authorLo, ECMen_US
dc.date.accessioned2012-08-08T08:27:07Z-
dc.date.available2012-08-08T08:27:07Z-
dc.date.issued2012en_US
dc.identifier.citationDental Materials, 2012, v. 28 n. 8, p. 903-908en_US
dc.identifier.issn0109-5641en_US
dc.identifier.urihttp://hdl.handle.net/10722/154722-
dc.description.abstractOBJECTIVE: To study the inhibitory effect of various commercially available concentrations of silver diamine fluoride (SDF) solutions on matrix metalloproteinases (MMPs). METHODS: Three SDF solutions with concentrations at 38%, 30% and 12% were studied. Two sodium fluoride (NaF) solutions at 10% and 3% were prepared, and they had the same fluoride ion concentrations as 38% and 12% SDF, respectively. Two silver nitrate (AgNO(3)) solutions at 42% and 13% were also prepared, and they had the same silver ion concentrations as 38% and 12% SDF, respectively. Ten samples of each experimental solution were used to study their inhibitory effect on three MMPs, which were MMP-2 (gelatinase A), MMP-8 (neutrophil collagenase) and MMP-9 (gelatinase B) using MMP assay kits. Positive control containing assay buffer at pH 9 and MMPs dilution was used to calculate the percentage inhibition. RESULTS: The percentage inhibition of 38%, 30% and 12% SDF on MMP-2 were 79%, 60% and 17%, respectively (p<0.001); on MMP-8 were 94%, 85% and 77%, respectively (p<0.001); on MMP-9 were 82%, 65% and 60%, respectively (p<0.001). The percentage inhibition on MMP-2, MMP-8 and MMP-9 by 38% SDF was significantly higher than the corresponding percentage inhibition by 10% NaF and 42% AgNO(3). SIGNIFICANCE: Greater inhibitory effect on MMPs was found with higher concentration of SDF solution. SDF had more inhibition on MMPs than solutions of NaF and AgNO(3) containing equivalent concentration of fluoride and silver ions, respectively.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/dentalen_US
dc.relation.ispartofDental Materialsen_US
dc.subjectArresten_US
dc.subjectCariesen_US
dc.subjectDentinen_US
dc.subjectMatrix metalloproteinasesen_US
dc.subjectSodium fluorideen_US
dc.titleThe inhibitory effects of silver diamine fluoride at different concentrations on matrix metalloproteinasesen_US
dc.typeArticleen_US
dc.identifier.emailMei, ML: leimei@hku.hken_US
dc.identifier.emailChu, CH: chchu@hku.hken_US
dc.identifier.emailYiu, CKY: ckyyiu@hkucc.hku.hken_US
dc.identifier.emailLo, ECM: hrdplcm@hkucc.hku.hk-
dc.identifier.authorityChu, CH=rp00022en_US
dc.identifier.authorityYiu, CKY=rp00018en_US
dc.identifier.authorityLo, ECM=rp00015en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.dental.2012.04.011en_US
dc.identifier.pmid22578660-
dc.identifier.scopuseid_2-s2.0-84863778390-
dc.identifier.hkuros202685-
dc.identifier.volume28-
dc.identifier.issue8-
dc.identifier.spage903-
dc.identifier.epage908-
dc.identifier.eissn1879-0097-
dc.identifier.isiWOS:000306485100017-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLo, ECM=7101705982en_US
dc.identifier.scopusauthoridYiu, CKY=7007115156en_US
dc.identifier.scopusauthoridChu, CH=7404345729en_US
dc.identifier.scopusauthoridLi, QL=55210294000en_US
dc.identifier.scopusauthoridMei, ML=36137431900en_US
dc.identifier.citeulike10665008-

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