File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Storage media enhance osteoclastogenic potential of human periodontal ligament cells via RANKL-independent signaling

TitleStorage media enhance osteoclastogenic potential of human periodontal ligament cells via RANKL-independent signaling
Authors
KeywordsInflammatory Cytokines
Osteoclast-Like Cells
Periodontal Ligament Cells
Raw 264.7
Storage Media
Issue Date2013
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EDT
Citation
Dental Traumatology, 2013, v. 29 n. 1, p. 59-65 How to Cite?
AbstractBackground: Hank's balanced salt solution (HBSS) and milk have gained wide acceptance as storage media for avulsed tooth. However, the effect of the media and storage time on the periodontal ligament (PDL) cells involvement in the development of root resorption is still unclear. The purpose of this study was to evaluate whether precultured PDL cells in HBSS, milk, or modified Eagle's medium alpha (α-MEM) would affect osteoclastogenesis. Materials and methods: PDL cells were precultured in HBSS, milk, or α-MEM for 1h or 6h before being co-cultured with RAW 264.7 cells for an additional 3days for mRNA analysis and 11days for osteoclastogenesis assay. Results: Cyclooxygenase-2 (COX-2) mRNA was detected immediately in PDL cells precultured in the three storage media. The expression was up-regulated markedly in all co-cultures when compared with RAW cells alone. As a result of the co-culture, interleukin-1β (IL-1β) expression was detectable in both PDL and RAW cells. TRAP+ multinucleated, osteoclast-like cells developed in all co-cultures; the number of TRAP+ cells was highest (P<0.05) in the co-cultures that PDL cells precultured in milk for 6h. The mRNA level of receptor activator of nuclear factor-kappa B ligand (RANKL) was not detected in PDL cells. Osteoprotegerin (OPG) mRNA expression reduced with increased preculture time, but the difference was not significant (P>0.05). Conclusions: PDL cells kept in the three storage media led to TRAP+ multinucleated, osteoclast-like cells formation via RANKL-independent signaling. The ability to induce osteoclastogenesis may be considered as one of the factors to evaluate the ability of storage medium to maintain PDL viability after tooth avulsion. © 2012 John Wiley & Sons A/S.
Persistent Identifierhttp://hdl.handle.net/10722/154716
ISSN
2015 Impact Factor: 1.327
2015 SCImago Journal Rankings: 0.955
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhan, Xen_US
dc.contributor.authorZhang, Cen_US
dc.contributor.authorDissanayaka, WLen_US
dc.contributor.authorCheung, GSPen_US
dc.contributor.authorJin, Len_US
dc.contributor.authorYang, Yen_US
dc.contributor.authorYan, Fen_US
dc.contributor.authorTong, EHYen_US
dc.date.accessioned2012-08-08T08:27:05Z-
dc.date.available2012-08-08T08:27:05Z-
dc.date.issued2013en_US
dc.identifier.citationDental Traumatology, 2013, v. 29 n. 1, p. 59-65en_US
dc.identifier.issn1600-4469en_US
dc.identifier.urihttp://hdl.handle.net/10722/154716-
dc.description.abstractBackground: Hank's balanced salt solution (HBSS) and milk have gained wide acceptance as storage media for avulsed tooth. However, the effect of the media and storage time on the periodontal ligament (PDL) cells involvement in the development of root resorption is still unclear. The purpose of this study was to evaluate whether precultured PDL cells in HBSS, milk, or modified Eagle's medium alpha (α-MEM) would affect osteoclastogenesis. Materials and methods: PDL cells were precultured in HBSS, milk, or α-MEM for 1h or 6h before being co-cultured with RAW 264.7 cells for an additional 3days for mRNA analysis and 11days for osteoclastogenesis assay. Results: Cyclooxygenase-2 (COX-2) mRNA was detected immediately in PDL cells precultured in the three storage media. The expression was up-regulated markedly in all co-cultures when compared with RAW cells alone. As a result of the co-culture, interleukin-1β (IL-1β) expression was detectable in both PDL and RAW cells. TRAP+ multinucleated, osteoclast-like cells developed in all co-cultures; the number of TRAP+ cells was highest (P<0.05) in the co-cultures that PDL cells precultured in milk for 6h. The mRNA level of receptor activator of nuclear factor-kappa B ligand (RANKL) was not detected in PDL cells. Osteoprotegerin (OPG) mRNA expression reduced with increased preculture time, but the difference was not significant (P>0.05). Conclusions: PDL cells kept in the three storage media led to TRAP+ multinucleated, osteoclast-like cells formation via RANKL-independent signaling. The ability to induce osteoclastogenesis may be considered as one of the factors to evaluate the ability of storage medium to maintain PDL viability after tooth avulsion. © 2012 John Wiley & Sons A/S.en_US
dc.languageengen_US
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EDTen_US
dc.relation.ispartofDental Traumatologyen_US
dc.subjectInflammatory Cytokinesen_US
dc.subjectOsteoclast-Like Cellsen_US
dc.subjectPeriodontal Ligament Cellsen_US
dc.subjectRaw 264.7en_US
dc.subjectStorage Mediaen_US
dc.titleStorage media enhance osteoclastogenic potential of human periodontal ligament cells via RANKL-independent signalingen_US
dc.typeArticleen_US
dc.identifier.emailZhang, C:zhangcf@hku.hken_US
dc.identifier.emailJin, L:ljjin@hkucc.hku.hken_US
dc.identifier.authorityZhang, C=rp01408en_US
dc.identifier.authorityJin, L=rp00028en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1600-9657.2012.01138.xen_US
dc.identifier.pmid22487464-
dc.identifier.scopuseid_2-s2.0-84871948403en_US
dc.identifier.hkuros207816-
dc.identifier.eissn1600-9657-
dc.identifier.isiWOS:000312997600011-
dc.publisher.placeDenmarken_US
dc.identifier.scopusauthoridZhan, X=54908728000en_US
dc.identifier.scopusauthoridZhang, C=7405494609en_US
dc.identifier.scopusauthoridDissanayaka, WL=36196419000en_US
dc.identifier.scopusauthoridCheung, GSP=55175400600en_US
dc.identifier.scopusauthoridJin, L=7403328850en_US
dc.identifier.scopusauthoridYang, Y=55175690700en_US
dc.identifier.scopusauthoridYan, F=12789764400en_US
dc.identifier.scopusauthoridTong, EHY=54908752000en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats