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Article: Enhancement of Secretory Aspartyl Protease production in biofilms of Candida albicans exposed to sub-inhibitory concentrations of fluconazole

TitleEnhancement of Secretory Aspartyl Protease production in biofilms of Candida albicans exposed to sub-inhibitory concentrations of fluconazole
Authors
Issue Date2011
PublisherWiley-Blackwell Verlag GmbH. The Journal's web site is located at http://www.blackwellpublishing.com/journals/MYC
Citation
Mycoses, 2011, v. 54 n. 3, p. 195-201 How to Cite?
AbstractThe production of Secretory Aspartyl Proteases (Sap) is an important virulence factor of Candida albicans. Many studies have shown that a challenge with sub-inhibitory concentrations of antifungals lead species of Candida to the secretion of higher concentrations of Sap. Nevertheless, published studies only reported the secretion of such enzymes by cells growing in planktonic phase, with few mention of biofilms. The present study evaluated the alterations in the secretion of Sap by C. albicans grown in biofilms and exposed to sub-inhibitory concentrations of fluconazole. The MICs for fluconazole of seven clinical strains were determined for planktonic cells. Biofilm and planktonic cells were grown in the presence of 1/2 MIC, 1/4 MIC, and no medication (control). The relative metabolic activity, indirectly related to cell loads, were estimated by the absorbance of reduced XTT and the Sap activity was evaluated by bovine albumin test. It was observed that 72h-old biofilms under the influence of 1/2 MIC had fewer cells than 1/4 MIC and control. The production of Sap was inversely proportional to the cell content, with higher secretion in 1/2 MIC, followed by 1/4 MIC and control. Biofilms of C. albicans challenged by sub-MICs of fluconazole tend to secrete higher quantities of Sap. © 2009 Blackwell Verlag GmbH.
Persistent Identifierhttp://hdl.handle.net/10722/154665
ISSN
2015 Impact Factor: 2.332
2015 SCImago Journal Rankings: 0.921
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMores, AUen_US
dc.contributor.authorSouza, RDen_US
dc.contributor.authorCavalca, Len_US
dc.contributor.authorDe Paula E Carvalho, Aen_US
dc.contributor.authorGursky, LCen_US
dc.contributor.authorRosa, RTen_US
dc.contributor.authorSamaranayake, LPen_US
dc.contributor.authorRosa, EARen_US
dc.date.accessioned2012-08-08T08:26:49Z-
dc.date.available2012-08-08T08:26:49Z-
dc.date.issued2011en_US
dc.identifier.citationMycoses, 2011, v. 54 n. 3, p. 195-201en_US
dc.identifier.issn0933-7407en_US
dc.identifier.urihttp://hdl.handle.net/10722/154665-
dc.description.abstractThe production of Secretory Aspartyl Proteases (Sap) is an important virulence factor of Candida albicans. Many studies have shown that a challenge with sub-inhibitory concentrations of antifungals lead species of Candida to the secretion of higher concentrations of Sap. Nevertheless, published studies only reported the secretion of such enzymes by cells growing in planktonic phase, with few mention of biofilms. The present study evaluated the alterations in the secretion of Sap by C. albicans grown in biofilms and exposed to sub-inhibitory concentrations of fluconazole. The MICs for fluconazole of seven clinical strains were determined for planktonic cells. Biofilm and planktonic cells were grown in the presence of 1/2 MIC, 1/4 MIC, and no medication (control). The relative metabolic activity, indirectly related to cell loads, were estimated by the absorbance of reduced XTT and the Sap activity was evaluated by bovine albumin test. It was observed that 72h-old biofilms under the influence of 1/2 MIC had fewer cells than 1/4 MIC and control. The production of Sap was inversely proportional to the cell content, with higher secretion in 1/2 MIC, followed by 1/4 MIC and control. Biofilms of C. albicans challenged by sub-MICs of fluconazole tend to secrete higher quantities of Sap. © 2009 Blackwell Verlag GmbH.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Verlag GmbH. The Journal's web site is located at http://www.blackwellpublishing.com/journals/MYCen_US
dc.relation.ispartofMycosesen_US
dc.subject.meshAntifungal Agents - Pharmacologyen_US
dc.subject.meshAspartic Acid Proteases - Secretionen_US
dc.subject.meshBiofilms - Drug Effectsen_US
dc.subject.meshCandida Albicans - Drug Effects - Enzymology - Metabolismen_US
dc.subject.meshFluconazole - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMicrobial Sensitivity Testsen_US
dc.subject.meshSpectrophotometryen_US
dc.subject.meshTetrazolium Salts - Metabolismen_US
dc.subject.meshVirulence Factors - Metabolismen_US
dc.titleEnhancement of Secretory Aspartyl Protease production in biofilms of Candida albicans exposed to sub-inhibitory concentrations of fluconazoleen_US
dc.typeArticleen_US
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1439-0507.2009.01793.xen_US
dc.identifier.pmid19878458-
dc.identifier.scopuseid_2-s2.0-79954452648en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79954452648&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume54en_US
dc.identifier.issue3en_US
dc.identifier.spage195en_US
dc.identifier.epage201en_US
dc.identifier.isiWOS:000289466500003-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridMores, AU=21739951100en_US
dc.identifier.scopusauthoridSouza, RD=49461844000en_US
dc.identifier.scopusauthoridCavalca, L=34771131200en_US
dc.identifier.scopusauthoridde Paula e Carvalho, A=26634977100en_US
dc.identifier.scopusauthoridGursky, LC=14827791100en_US
dc.identifier.scopusauthoridRosa, RT=35576111200en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US
dc.identifier.scopusauthoridRosa, EAR=7004952748en_US
dc.identifier.citeulike9156349-

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