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Article: Biodegradable polycaprolactone-chitosan three-dimensional scaffolds fabricated by melt stretching and multilayer deposition for bone tissue engineering: Assessment of the physical properties and cellular response

TitleBiodegradable polycaprolactone-chitosan three-dimensional scaffolds fabricated by melt stretching and multilayer deposition for bone tissue engineering: Assessment of the physical properties and cellular response
Authors
Issue Date2011
PublisherInstitute of Physics Publishing Ltd.. The Journal's web site is located at http://www.iop.org/EJ/journal/BMM
Citation
Biomedical Materials, 2011, v. 6 n. 1, article no. 015009 How to Cite?
AbstractFabrication of polycaprolactone (PCL)-chitosan (CS) three-dimensional (3D) scaffolds using the novel technique of melt stretching and multilayer deposition was introduced. In brief, firstly, the PCL-CS monofilaments containing 0% (pure PCL), 10%, 20% and 30% CS by weight were fabricated by melting and stretching processes. Secondly, the desired multilayer (3D) scaffolds were fabricated by arranging and depositing the filaments. Physical properties of the filaments and the scaffolds were evaluated. MC3T3-E1 cell lines were seeded on the scaffolds to assess their proliferation. A typical micro-groove pattern was found on the surfaces of pure PCL filaments due to stretching. The filaments of PCL-30%CS had the highest tendency of fracture during stretching and could not be used to form the scaffold. Increasing CS proportions tended to reduce the micro-groove pattern, surface roughness, tensile strength and elasticity of the filaments, whilst compressive strength of the PCL-CS scaffolds was not affected. The average pore size and porosity of the scaffolds were 536.90 ± 17.91 μm and 45.99 ± 2.8% respectively. Over 60 days, degradation of the scaffolds gradually increased (p > 0.05). The more CS containing scaffolds were found to increase in water uptake, but decrease in degradation rate. During the culture period, the growth of the cells in PCL-CS groups was significantly higher than in the pure PCL group (p < 0.05). On culture-day 21, the growth in the PCL-20%CS group was significantly higher than the other groups (p < 0.05). In conclusion, the PCL-20%CS scaffolds obtained the optimum results in terms of physical properties and cellular response. © 2011 IOP Publishing Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/154655
ISSN
2015 Impact Factor: 3.361
2015 SCImago Journal Rankings: 0.936
ISI Accession Number ID
Funding AgencyGrant Number
Faculty of Graduate Studies, Prince of Songkla University, Hatyai, Songkhla, Thailand
Funding Information:

The study was supported by grants from the Faculty of Graduate Studies, Prince of Songkla University, Hatyai, Songkhla, Thailand.

References

 

DC FieldValueLanguage
dc.contributor.authorThuaksuban, Nen_US
dc.contributor.authorNuntanaranont, Ten_US
dc.contributor.authorPattanachot, Wen_US
dc.contributor.authorSuttapreyasri, Sen_US
dc.contributor.authorCheung, LKen_US
dc.date.accessioned2012-08-08T08:26:44Z-
dc.date.available2012-08-08T08:26:44Z-
dc.date.issued2011en_US
dc.identifier.citationBiomedical Materials, 2011, v. 6 n. 1, article no. 015009en_US
dc.identifier.issn1748-6041en_US
dc.identifier.urihttp://hdl.handle.net/10722/154655-
dc.description.abstractFabrication of polycaprolactone (PCL)-chitosan (CS) three-dimensional (3D) scaffolds using the novel technique of melt stretching and multilayer deposition was introduced. In brief, firstly, the PCL-CS monofilaments containing 0% (pure PCL), 10%, 20% and 30% CS by weight were fabricated by melting and stretching processes. Secondly, the desired multilayer (3D) scaffolds were fabricated by arranging and depositing the filaments. Physical properties of the filaments and the scaffolds were evaluated. MC3T3-E1 cell lines were seeded on the scaffolds to assess their proliferation. A typical micro-groove pattern was found on the surfaces of pure PCL filaments due to stretching. The filaments of PCL-30%CS had the highest tendency of fracture during stretching and could not be used to form the scaffold. Increasing CS proportions tended to reduce the micro-groove pattern, surface roughness, tensile strength and elasticity of the filaments, whilst compressive strength of the PCL-CS scaffolds was not affected. The average pore size and porosity of the scaffolds were 536.90 ± 17.91 μm and 45.99 ± 2.8% respectively. Over 60 days, degradation of the scaffolds gradually increased (p > 0.05). The more CS containing scaffolds were found to increase in water uptake, but decrease in degradation rate. During the culture period, the growth of the cells in PCL-CS groups was significantly higher than in the pure PCL group (p < 0.05). On culture-day 21, the growth in the PCL-20%CS group was significantly higher than the other groups (p < 0.05). In conclusion, the PCL-20%CS scaffolds obtained the optimum results in terms of physical properties and cellular response. © 2011 IOP Publishing Ltd.en_US
dc.languageengen_US
dc.publisherInstitute of Physics Publishing Ltd.. The Journal's web site is located at http://www.iop.org/EJ/journal/BMMen_US
dc.relation.ispartofBiomedical Materialsen_US
dc.rightsBiomedical Materials (Bristol). Copyright © Institute of Physics Publishing Ltd..-
dc.subject.mesh3T3 Cellsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiomechanicsen_US
dc.subject.meshBone Substitutes - Chemistryen_US
dc.subject.meshCell Proliferationen_US
dc.subject.meshChitosan - Chemistryen_US
dc.subject.meshElastic Modulusen_US
dc.subject.meshHot Temperatureen_US
dc.subject.meshMaterials Testingen_US
dc.subject.meshMiceen_US
dc.subject.meshMicroscopy, Atomic Forceen_US
dc.subject.meshMicroscopy, Electron, Scanningen_US
dc.subject.meshOsteoblasts - Cytologyen_US
dc.subject.meshPolyesters - Chemistryen_US
dc.subject.meshPorosityen_US
dc.subject.meshTissue Engineering - Instrumentation - Methodsen_US
dc.subject.meshTissue Scaffolds - Chemistryen_US
dc.titleBiodegradable polycaprolactone-chitosan three-dimensional scaffolds fabricated by melt stretching and multilayer deposition for bone tissue engineering: Assessment of the physical properties and cellular responseen_US
dc.typeArticleen_US
dc.identifier.emailCheung, LK:lkcheung@hkucc.hku.hken_US
dc.identifier.authorityCheung, LK=rp00013en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1088/1748-6041/6/1/015009en_US
dc.identifier.pmid21205996-
dc.identifier.scopuseid_2-s2.0-79551491920en_US
dc.identifier.hkuros185343-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79551491920&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume6en_US
dc.identifier.issue1, article no. 015009en_US
dc.identifier.isiWOS:000286221300010-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridThuaksuban, N=36702104000en_US
dc.identifier.scopusauthoridNuntanaranont, T=7801655400en_US
dc.identifier.scopusauthoridPattanachot, W=36915567300en_US
dc.identifier.scopusauthoridSuttapreyasri, S=13104959000en_US
dc.identifier.scopusauthoridCheung, LK=7102302747en_US

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