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Article: Evaluation of Ellagic acid on the activities of oral bacteria with the use of adenosine triphosphate (ATP) bioluminescence assay

TitleEvaluation of Ellagic acid on the activities of oral bacteria with the use of adenosine triphosphate (ATP) bioluminescence assay
Authors
KeywordsAdenosine Triphosphate Bioluminescence Assay
Ellagic Acid
Galla Chinensis
Oral Bacteria
Issue Date2010
PublisherAcademic Journals. The Journal's web site is located at http://www.academicjournals.org/AJB/index.htm
Citation
African Journal Of Biotechnology, 2010, v. 9 n. 25, p. 3938-3943 How to Cite?
AbstractEllagic acid, a natural herb extract from Galla Chinensis in traditional Chinese medicine, shows antimicrobial activity to certain bacteria. The present study evaluated the effect of Ellagic acid on the growth of oral bacteria as well as their generation of water-insoluble glucan and adhesion to saliva-coated hydroxyapatite (S-HA) beads. Streptococcus mutans ATCC 25175, Streptococcus sanguis ATCC 10556, Streptococcus salivarius ATCC 25975, Actinomyces naeslundii ATCC 12104, Actinomyces viscosus ATCC 15987, Lactobacillus rhamnosus ATCC 53103, Porphyromonas gingivalis ATCC 33277 and Bacteroides forsythus ATCC 43037 were the bacterial cell lines used in this study. Antibacterial activity of Ellagic acid was determined by using adenosine triphosphate (ATP) bioluminescence assay at various concentrations from 0.125 to 8 mg/ml. Anthrone method was used to evaluate the level of water-insoluble glucan generated by oral bacteria. The numbers of 3H-thymidine labeled bacteria attached to S-HA was counted by scintillation counting method. Sprague Dawley rats were orally fed with 0.5mg/mL ellagic acid for 28 days and their behaviours and excretions were monitored. Ellagic acid reduced bacterial metabolic rates and inhibited the growth of the tested bacterial strains. The water-insoluble glucan generated by S. mutans and its adhesion to S-HA were reduced. Ellagic acid demonstrated no toxicity in animals fed for 28 days. Ellagic acid might be a promising compound for the development of antimicrobial agents against oral pathogens in human, thereby reducing the incidence of dental caries. © 2010 Academic Journals.
Persistent Identifierhttp://hdl.handle.net/10722/154621
ISSN
2010 Impact Factor: 0.573
2015 SCImago Journal Rankings: 0.295
References

 

DC FieldValueLanguage
dc.contributor.authorLoo, WTYen_US
dc.contributor.authorJin, LJen_US
dc.contributor.authorCheung, MNBen_US
dc.contributor.authorChow, LWCen_US
dc.date.accessioned2012-08-08T08:26:32Z-
dc.date.available2012-08-08T08:26:32Z-
dc.date.issued2010en_US
dc.identifier.citationAfrican Journal Of Biotechnology, 2010, v. 9 n. 25, p. 3938-3943en_US
dc.identifier.issn1684-5315en_US
dc.identifier.urihttp://hdl.handle.net/10722/154621-
dc.description.abstractEllagic acid, a natural herb extract from Galla Chinensis in traditional Chinese medicine, shows antimicrobial activity to certain bacteria. The present study evaluated the effect of Ellagic acid on the growth of oral bacteria as well as their generation of water-insoluble glucan and adhesion to saliva-coated hydroxyapatite (S-HA) beads. Streptococcus mutans ATCC 25175, Streptococcus sanguis ATCC 10556, Streptococcus salivarius ATCC 25975, Actinomyces naeslundii ATCC 12104, Actinomyces viscosus ATCC 15987, Lactobacillus rhamnosus ATCC 53103, Porphyromonas gingivalis ATCC 33277 and Bacteroides forsythus ATCC 43037 were the bacterial cell lines used in this study. Antibacterial activity of Ellagic acid was determined by using adenosine triphosphate (ATP) bioluminescence assay at various concentrations from 0.125 to 8 mg/ml. Anthrone method was used to evaluate the level of water-insoluble glucan generated by oral bacteria. The numbers of 3H-thymidine labeled bacteria attached to S-HA was counted by scintillation counting method. Sprague Dawley rats were orally fed with 0.5mg/mL ellagic acid for 28 days and their behaviours and excretions were monitored. Ellagic acid reduced bacterial metabolic rates and inhibited the growth of the tested bacterial strains. The water-insoluble glucan generated by S. mutans and its adhesion to S-HA were reduced. Ellagic acid demonstrated no toxicity in animals fed for 28 days. Ellagic acid might be a promising compound for the development of antimicrobial agents against oral pathogens in human, thereby reducing the incidence of dental caries. © 2010 Academic Journals.en_US
dc.languageengen_US
dc.publisherAcademic Journals. The Journal's web site is located at http://www.academicjournals.org/AJB/index.htmen_US
dc.relation.ispartofAfrican Journal of Biotechnologyen_US
dc.subjectAdenosine Triphosphate Bioluminescence Assayen_US
dc.subjectEllagic Aciden_US
dc.subjectGalla Chinensisen_US
dc.subjectOral Bacteriaen_US
dc.titleEvaluation of Ellagic acid on the activities of oral bacteria with the use of adenosine triphosphate (ATP) bioluminescence assayen_US
dc.typeArticleen_US
dc.identifier.emailJin, LJ:ljjin@hkucc.hku.hken_US
dc.identifier.authorityJin, LJ=rp00028en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-77954874816en_US
dc.identifier.hkuros180183-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77954874816&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume9en_US
dc.identifier.issue25en_US
dc.identifier.spage3938en_US
dc.identifier.epage3943en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLoo, WTY=7003567474en_US
dc.identifier.scopusauthoridJin, LJ=7403328850en_US
dc.identifier.scopusauthoridCheung, MNB=7201897548en_US
dc.identifier.scopusauthoridChow, LWC=34975216600en_US

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