File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Chlorhexidine release and antibacterial properties of chlorhexidine- incorporated polymethyl methacrylate-based resin cement

TitleChlorhexidine release and antibacterial properties of chlorhexidine- incorporated polymethyl methacrylate-based resin cement
Authors
KeywordsAgar diffusion test
Antibacterial effects
Chlorhexidine
Direct contact test
HPLC
Resin cement
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0021-9304:1/
Citation
Journal Of Biomedical Materials Research - Part B Applied Biomaterials, 2010, v. 94 n. 1, p. 134-140 How to Cite?
AbstractThis study evaluated chlorhexidine release from experimental, chlorhexidine-incorporated polymethyl methacrylate (PMMA)-based resin cements prepared from Super-Bond C&B (Sun Medical) and examined the antimicrobial activity against Streptococcus mutans and Enterococcus faecalis. Chlorhexidine diacetate was added into PMMA polymer to obtain chlorhexidine concentration of 0.0, 1.0, 2.0, 3.0, and 4.0 wt %. Chlorhexidine-incorporated, cured resin disks were immersed in distilled water at 37°C for 5 weeks, and the chlorhexidine release was analyzed by high-performance liquid chromatography. The antibacterial effect of freshly mixed resin cements was examined using the agar diffusion test. For the direct contact test, the wells (n = 6) of microtiter plates were coated with cements. The coated wells were aged up to 3 weeks prior to the placement of bacterial suspensions directly on cured cements. The 3.0 and 4.0% chlorhexidine-incorporated cement exhibited chlorhexidine release for 5 weeks; however, more than 98% of chlorhexidine was retained in resin matrix. No release was detected from the 1.0 and 2.0% incorporated cement at 1 week and 2 weeks, respectively. The agar diffusion test failed to detect antibacterial effects against Enterococcus faecalis, whereas the direct contact test revealed the antibacterial effect of 3.0 and 4.0% incorporated cements against each microbe for 2 weeks. The 3.0 and 4.0% chlorhexidine-incorporated resin cement possessed prolonged chlorhexidine release and antibacterial properties for 2 weeks. © 2010 Wiley Periodicals, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/154617
ISSN
2015 Impact Factor: 2.881
2015 SCImago Journal Rankings: 0.784
ISI Accession Number ID
Funding AgencyGrant Number
Faculty of Dentistry, University of Hong Kong10207401/14207/08004/323/01
10204604/07840/08004/324/01
10207821.14207.8004.324.01
Funding Information:

Contract grant sponsor: Faculty of Dentistry, University of Hong Kong; contract grant numbers: 10207401/14207/08004/323/01, 10204604/07840/08004/324/01, 10207821.14207.8004.324.01

References

 

DC FieldValueLanguage
dc.contributor.authorHiraishi, Nen_HK
dc.contributor.authorYiu, CKYen_HK
dc.contributor.authorKing, NMen_HK
dc.contributor.authorTay, FRen_HK
dc.date.accessioned2012-08-08T08:26:30Z-
dc.date.available2012-08-08T08:26:30Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Biomedical Materials Research - Part B Applied Biomaterials, 2010, v. 94 n. 1, p. 134-140en_HK
dc.identifier.issn1552-4973en_HK
dc.identifier.urihttp://hdl.handle.net/10722/154617-
dc.description.abstractThis study evaluated chlorhexidine release from experimental, chlorhexidine-incorporated polymethyl methacrylate (PMMA)-based resin cements prepared from Super-Bond C&B (Sun Medical) and examined the antimicrobial activity against Streptococcus mutans and Enterococcus faecalis. Chlorhexidine diacetate was added into PMMA polymer to obtain chlorhexidine concentration of 0.0, 1.0, 2.0, 3.0, and 4.0 wt %. Chlorhexidine-incorporated, cured resin disks were immersed in distilled water at 37°C for 5 weeks, and the chlorhexidine release was analyzed by high-performance liquid chromatography. The antibacterial effect of freshly mixed resin cements was examined using the agar diffusion test. For the direct contact test, the wells (n = 6) of microtiter plates were coated with cements. The coated wells were aged up to 3 weeks prior to the placement of bacterial suspensions directly on cured cements. The 3.0 and 4.0% chlorhexidine-incorporated cement exhibited chlorhexidine release for 5 weeks; however, more than 98% of chlorhexidine was retained in resin matrix. No release was detected from the 1.0 and 2.0% incorporated cement at 1 week and 2 weeks, respectively. The agar diffusion test failed to detect antibacterial effects against Enterococcus faecalis, whereas the direct contact test revealed the antibacterial effect of 3.0 and 4.0% incorporated cements against each microbe for 2 weeks. The 3.0 and 4.0% chlorhexidine-incorporated resin cement possessed prolonged chlorhexidine release and antibacterial properties for 2 weeks. © 2010 Wiley Periodicals, Inc.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0021-9304:1/en_HK
dc.relation.ispartofJournal of Biomedical Materials Research - Part B Applied Biomaterialsen_HK
dc.subjectAgar diffusion testen_HK
dc.subjectAntibacterial effectsen_HK
dc.subjectChlorhexidineen_HK
dc.subjectDirect contact testen_HK
dc.subjectHPLCen_HK
dc.subjectResin cementen_HK
dc.subject.meshAnti-Bacterial Agents - Chemistry - Pharmacologyen_US
dc.subject.meshAnti-Infective Agents, Local - Chemistry - Pharmacologyen_US
dc.subject.meshBiocompatible Materials - Chemistry - Pharmacologyen_US
dc.subject.meshBoron Compounds - Chemistry - Pharmacologyen_US
dc.subject.meshChlorhexidine - Chemistry - Metabolism - Pharmacologyen_US
dc.subject.meshEnterococcus Faecalis - Drug Effects - Growth & Developmenten_US
dc.subject.meshHumansen_US
dc.subject.meshMaterials Testingen_US
dc.subject.meshMethacrylates - Chemistry - Pharmacologyen_US
dc.subject.meshMethylmethacrylates - Chemistry - Pharmacologyen_US
dc.subject.meshMicrobial Sensitivity Testsen_US
dc.subject.meshPolymethyl Methacrylate - Chemistry - Pharmacologyen_US
dc.subject.meshResin Cements - Chemistry - Pharmacologyen_US
dc.subject.meshStreptococcus Mutans - Drug Effects - Growth & Developmenten_US
dc.titleChlorhexidine release and antibacterial properties of chlorhexidine- incorporated polymethyl methacrylate-based resin cementen_HK
dc.typeArticleen_HK
dc.identifier.emailYiu, CKY: ckyyiu@hkucc.hku.hken_HK
dc.identifier.emailKing, NM: hhdbknm@hkucc.hku.hken_HK
dc.identifier.authorityYiu, CKY=rp00018en_HK
dc.identifier.authorityKing, NM=rp00006en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/jbm.b.31633en_HK
dc.identifier.pmid20524187-
dc.identifier.scopuseid_2-s2.0-77954111691en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77954111691&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume94en_HK
dc.identifier.issue1en_HK
dc.identifier.spage134en_HK
dc.identifier.epage140en_HK
dc.identifier.isiWOS:000278697400015-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHiraishi, N=8925934400en_HK
dc.identifier.scopusauthoridYiu, CKY=7007115156en_HK
dc.identifier.scopusauthoridKing, NM=7201762850en_HK
dc.identifier.scopusauthoridTay, FR=7102091962en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats