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Article: Proteomics of drug resistance in candida glabrata biofilms

TitleProteomics of drug resistance in candida glabrata biofilms
Authors
Keywords2-D DIGE
Biofilm
Candida glabrata
Microbiology
Stress response
Issue Date2010
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics
Citation
Proteomics, 2010, v. 10 n. 7, p. 1444-1454 How to Cite?
AbstractCandida glabrata is a fungal pathogen that causes a variety of mucosal and systemic infections among compromised patient populations with higher mortality rates. Previous studies have shown that biofilm mode of the growth of the fungus is highly resistant to antifungal agents compared with the free-floating or planktonic mode of growth. Therefore, in the present study, we used 2-D DIGE to evaluate the differential proteomic profiles of C. glabrata under planktonic and biofilm modes of growth. Candida glabrata biofilms were developed on polystyrene surfaces and age-matched planktonic cultures were obtained in parallel. Initially, biofilm architecture, viability, and antifungal susceptibility were evaluated. Differentially expressed proteins more than 1.5-fold in DIGE analysis were subjected to MS/MS. The transcriptomic regulation of these biomarkers was evaluated by quantitative real-time PCR. Candida glabrata biofilms were highly resistant to the antifungals and biocides compared with the planktonic mode of growth. Candida glabrata biofilm proteome when compared with its planktonic proteome showed upregulation of stress response proteins, while glycolysis enzymes were downregulated. Similar trend could be observed at transcriptomic level. In conclusion, C. glabrata biofilms possess higher amount of stress response proteins, which may potentially contribute to the higher antifungal resistance seen in C. glabrata biofilms. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA.
Persistent Identifierhttp://hdl.handle.net/10722/154605
ISSN
2015 Impact Factor: 4.079
2015 SCImago Journal Rankings: 1.476
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants Council, RGCHKU 7624/06M
Funding Information:

We thank Alan Wong, Lawrence Luk, and Priscilla Leung for technical assistance and Dr. Trevor Lane for editorial assistance. This work was supported by the Hong Kong Research Grants Council, RGC no. HKU 7624/06M.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorJayampath Seneviratne, Cen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorJin, Len_HK
dc.contributor.authorAbiko, Yen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2012-08-08T08:26:28Z-
dc.date.available2012-08-08T08:26:28Z-
dc.date.issued2010en_HK
dc.identifier.citationProteomics, 2010, v. 10 n. 7, p. 1444-1454en_HK
dc.identifier.issn1615-9853en_HK
dc.identifier.urihttp://hdl.handle.net/10722/154605-
dc.description.abstractCandida glabrata is a fungal pathogen that causes a variety of mucosal and systemic infections among compromised patient populations with higher mortality rates. Previous studies have shown that biofilm mode of the growth of the fungus is highly resistant to antifungal agents compared with the free-floating or planktonic mode of growth. Therefore, in the present study, we used 2-D DIGE to evaluate the differential proteomic profiles of C. glabrata under planktonic and biofilm modes of growth. Candida glabrata biofilms were developed on polystyrene surfaces and age-matched planktonic cultures were obtained in parallel. Initially, biofilm architecture, viability, and antifungal susceptibility were evaluated. Differentially expressed proteins more than 1.5-fold in DIGE analysis were subjected to MS/MS. The transcriptomic regulation of these biomarkers was evaluated by quantitative real-time PCR. Candida glabrata biofilms were highly resistant to the antifungals and biocides compared with the planktonic mode of growth. Candida glabrata biofilm proteome when compared with its planktonic proteome showed upregulation of stress response proteins, while glycolysis enzymes were downregulated. Similar trend could be observed at transcriptomic level. In conclusion, C. glabrata biofilms possess higher amount of stress response proteins, which may potentially contribute to the higher antifungal resistance seen in C. glabrata biofilms. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA.en_HK
dc.languageengen_US
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomicsen_HK
dc.relation.ispartofProteomicsen_HK
dc.subject2-D DIGEen_HK
dc.subjectBiofilmen_HK
dc.subjectCandida glabrataen_HK
dc.subjectMicrobiologyen_HK
dc.subjectStress responseen_HK
dc.subject.meshBiofilmsen_US
dc.subject.meshCandida Glabrata - Drug Effects - Genetics - Metabolism - Physiologyen_US
dc.subject.meshDrug Resistance, Fungalen_US
dc.subject.meshElectrophoresis, Gel, Two-Dimensionalen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshProteomics - Methodsen_US
dc.subject.meshStress, Physiologicalen_US
dc.titleProteomics of drug resistance in candida glabrata biofilmsen_HK
dc.typeArticleen_HK
dc.identifier.emailJayampath Seneviratne, C: jaya@hku.hken_HK
dc.identifier.emailWang, Y: yuwanghk@hku.hken_HK
dc.identifier.emailJin, L: ljjin@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_HK
dc.identifier.authorityJayampath Seneviratne, C=rp01372en_HK
dc.identifier.authorityWang, Y=rp00239en_HK
dc.identifier.authorityJin, L=rp00028en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/pmic.200900611en_HK
dc.identifier.pmid20127690-
dc.identifier.scopuseid_2-s2.0-77950657284en_HK
dc.identifier.hkuros170947-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77950657284&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume10en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1444en_HK
dc.identifier.epage1454en_HK
dc.identifier.eissn1615-9861-
dc.identifier.isiWOS:000277253200010-
dc.publisher.placeGermanyen_HK
dc.relation.projectCandida biofilms: molecular mechanisms and clinical implications-
dc.identifier.scopusauthoridJayampath Seneviratne, C=6701897753en_HK
dc.identifier.scopusauthoridWang, Y=34973733700en_HK
dc.identifier.scopusauthoridJin, L=7403328850en_HK
dc.identifier.scopusauthoridAbiko, Y=23491243700en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.customcontrol.immutablecsl 150127-

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