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Article: Localization of matrix metalloproteinases (MMPs-2, 8, 9 and 20) in normal and carious dentine

TitleLocalization of matrix metalloproteinases (MMPs-2, 8, 9 and 20) in normal and carious dentine
Authors
Issue Date2009
PublisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0045-0421
Citation
Australian Dental Journal, 2009, v. 54 n. 4, p. 347-354 How to Cite?
AbstractBackground: Dentine matrix metalloproteinases (MMPs) may participate in the destruction of dentine following demineralization by bacterial acids. This study investigated the localization of MMPs in carious dentine. Methods: Frozen sections of dentine caries were prepared without demineralization and immersed in monoclonal antibody against MMP-2, -8, -9 and -20. The sections were labelled by IgG conjugated with gold colloidal particles, and observed under FE-SEM. Labelling indexes (number of gold particles /μm 2) of outer and inner carious dentine, respectively, with and without bacterial infection, were compared with that of normal dentine. Results: MMP-2 was distributed in both carious and normal dentine; the level of MMP-2 showed no significant difference among the outer caries, inner caries, and normal dentine. The labelling indexes of MMP-8 and MMP-9 both significantly decreased at the inner carious dentine compared with the level of normal dentine, but intensified again at the outer caries region. The labelling index of MMP-20 was the highest at normal dentine. Conclusions: The localization of MMPs was visibly detected using immunogold labelling. The localization of MMP-2 showed no significant difference among the three regions, while MMP-8 and MMP-9 showed significant reduction at the inner caries layer, and MMP-20 reduced toward the outer caries. © 2009 Australian Dental Association.
Persistent Identifierhttp://hdl.handle.net/10722/154600
ISSN
2015 Impact Factor: 1.272
2015 SCImago Journal Rankings: 0.482
ISI Accession Number ID
Funding AgencyGrant Number
Japan Society for the Promotion of Science18592084
Funding Information:

This study was financially supported by grants from the Japan Society for the Promotion of Science (No. 18592084).

References

 

DC FieldValueLanguage
dc.contributor.authorShimada, Yen_US
dc.contributor.authorIchinose, Sen_US
dc.contributor.authorSadr, Aen_US
dc.contributor.authorBurrow, MFen_US
dc.contributor.authorTagami, Jen_US
dc.date.accessioned2012-08-08T08:26:25Z-
dc.date.available2012-08-08T08:26:25Z-
dc.date.issued2009en_US
dc.identifier.citationAustralian Dental Journal, 2009, v. 54 n. 4, p. 347-354en_US
dc.identifier.issn0045-0421en_US
dc.identifier.urihttp://hdl.handle.net/10722/154600-
dc.description.abstractBackground: Dentine matrix metalloproteinases (MMPs) may participate in the destruction of dentine following demineralization by bacterial acids. This study investigated the localization of MMPs in carious dentine. Methods: Frozen sections of dentine caries were prepared without demineralization and immersed in monoclonal antibody against MMP-2, -8, -9 and -20. The sections were labelled by IgG conjugated with gold colloidal particles, and observed under FE-SEM. Labelling indexes (number of gold particles /μm 2) of outer and inner carious dentine, respectively, with and without bacterial infection, were compared with that of normal dentine. Results: MMP-2 was distributed in both carious and normal dentine; the level of MMP-2 showed no significant difference among the outer caries, inner caries, and normal dentine. The labelling indexes of MMP-8 and MMP-9 both significantly decreased at the inner carious dentine compared with the level of normal dentine, but intensified again at the outer caries region. The labelling index of MMP-20 was the highest at normal dentine. Conclusions: The localization of MMPs was visibly detected using immunogold labelling. The localization of MMP-2 showed no significant difference among the three regions, while MMP-8 and MMP-9 showed significant reduction at the inner caries layer, and MMP-20 reduced toward the outer caries. © 2009 Australian Dental Association.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0045-0421en_US
dc.relation.ispartofAustralian Dental Journalen_US
dc.subject.meshAdulten_US
dc.subject.meshChi-Square Distributionen_US
dc.subject.meshDental Caries - Enzymologyen_US
dc.subject.meshDentin - Enzymology - Ultrastructureen_US
dc.subject.meshGolden_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistry - Methodsen_US
dc.subject.meshMatrix Metalloproteinases - Analysisen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMolar, Thirden_US
dc.subject.meshStatistics, Nonparametricen_US
dc.titleLocalization of matrix metalloproteinases (MMPs-2, 8, 9 and 20) in normal and carious dentineen_US
dc.typeArticleen_US
dc.identifier.emailBurrow, MF:mfburr58@hku.hken_US
dc.identifier.authorityBurrow, MF=rp01306en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1834-7819.2009.01161.xen_US
dc.identifier.pmid20415934-
dc.identifier.scopuseid_2-s2.0-77949349586en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77949349586&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume54en_US
dc.identifier.issue4en_US
dc.identifier.spage347en_US
dc.identifier.epage354en_US
dc.identifier.isiWOS:000272160600011-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridShimada, Y=7402123770en_US
dc.identifier.scopusauthoridIchinose, S=7006841449en_US
dc.identifier.scopusauthoridSadr, A=35276097500en_US
dc.identifier.scopusauthoridBurrow, MF=7005876730en_US
dc.identifier.scopusauthoridTagami, J=7005967527en_US

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