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Article: Isolation and characterization of Candida kefyr orotidine-5′- phosphate decarboxylase (URA3) gene

TitleIsolation and characterization of Candida kefyr orotidine-5′- phosphate decarboxylase (URA3) gene
Authors
Issue Date2010
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/3895
Citation
Yeast, 2010, v. 27 n. 1, p. 53-58 How to Cite?
AbstractCandida kefyr is a common yeast species that can be found in fermented milk and cheeses. As a first step to developing a gene transfer system for C. kefyr, the orotidine-5′-phosphate decarboxylase (URA3) gene was cloned, using degenerate PCR and genome walking. The uninterrupted open reading frame of the C. kefyr URA3 gene spans 801 bp, corresponding to 267 amino acid residues. The functionality of the gene was confirmed by complementation of ura3 auxotrophs of C. albicans and Saccharomyces cerevisiae. Phylogenetic analysis of the deduced amino acid sequence indicated that it shares a high degree of homology with other Candida URA3 homologues. The GenBank Accession No. of the C. kefyr URA3 gene is FJ914763. Copyright © 2009 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/154595
ISSN
2015 Impact Factor: 2.259
2015 SCImago Journal Rankings: 0.983
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTsang, PWKen_US
dc.contributor.authorWong, KSen_US
dc.contributor.authorChu, JKMen_US
dc.date.accessioned2012-08-08T08:26:23Z-
dc.date.available2012-08-08T08:26:23Z-
dc.date.issued2010en_US
dc.identifier.citationYeast, 2010, v. 27 n. 1, p. 53-58en_US
dc.identifier.issn0749-503Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/154595-
dc.description.abstractCandida kefyr is a common yeast species that can be found in fermented milk and cheeses. As a first step to developing a gene transfer system for C. kefyr, the orotidine-5′-phosphate decarboxylase (URA3) gene was cloned, using degenerate PCR and genome walking. The uninterrupted open reading frame of the C. kefyr URA3 gene spans 801 bp, corresponding to 267 amino acid residues. The functionality of the gene was confirmed by complementation of ura3 auxotrophs of C. albicans and Saccharomyces cerevisiae. Phylogenetic analysis of the deduced amino acid sequence indicated that it shares a high degree of homology with other Candida URA3 homologues. The GenBank Accession No. of the C. kefyr URA3 gene is FJ914763. Copyright © 2009 John Wiley & Sons, Ltd.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/3895en_US
dc.relation.ispartofYeasten_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshCandida - Enzymologyen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshFungal Proteins - Genetics - Metabolismen_US
dc.subject.meshGene Expression Regulation, Enzymologic - Physiologyen_US
dc.subject.meshGene Expression Regulation, Fungal - Physiologyen_US
dc.subject.meshOrotidine-5'-Phosphate Decarboxylase - Genetics - Metabolismen_US
dc.subject.meshPhylogenyen_US
dc.titleIsolation and characterization of Candida kefyr orotidine-5′- phosphate decarboxylase (URA3) geneen_US
dc.typeArticleen_US
dc.identifier.emailTsang, PWK:pwktsang@hku.hken_US
dc.identifier.authorityTsang, PWK=rp01388en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/yea.1732en_US
dc.identifier.pmid19908199-
dc.identifier.scopuseid_2-s2.0-73449094534en_US
dc.identifier.hkuros171294-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-73449094534&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume27en_US
dc.identifier.issue1en_US
dc.identifier.spage53en_US
dc.identifier.epage58en_US
dc.identifier.isiWOS:000273679100005-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridTsang, PWK=8334953500en_US
dc.identifier.scopusauthoridWong, KS=35191126000en_US
dc.identifier.scopusauthoridChu, JKM=35749436900en_US

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