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Article: Enhancement of Candida albicans virulence after exposition to cigarette mainstream smoke

TitleEnhancement of Candida albicans virulence after exposition to cigarette mainstream smoke
Authors
Issue Date2009
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0301-486X
Citation
Mycopathologia, 2009, v. 168 n. 5, p. 227-235 How to Cite?
AbstractThe habit of cigarette smoking is associated with higher oral candidal carriage and possible predisposition to oral candidosis. The effects of exposure to smoke on the virulence properties of oral yeasts remain obscure. Hence, we showed in vitro the effect of cigarette smoke condensate (CSC) on ten clinical isolates of Candida albicans obtained from nonsmoking volunteers, as well the type-strain CBS562. CSC was generated by complete burn of five commercial cigarettes in an in-house smoking machine and used to prepare the culture broth in which the strains were grown. In 24-h intervals (T24, T48, and T72), the cells were harvested, washed, subcultured, and the resultant growth were evaluated for possible variations for secreted aspartyl protease, phospholipase, chondroitinase, and hemolysins, adhesion to acrylic and cell surface hydrophobicity (CSH). The results indicated a temporal increase in the secretion rates of enzymes, particularly when yeast cells were exposed to CSC for 48-72h (P < 0.05). Similarly, adhesion to acrylic and CSH increased with exposure period (P < 0.05). Based on foregoing, we concluded that CSC may promote significant enhance in the secretion of candidal histolytic enzymes and adherence to denture surfaces, thereby promoting oral yeast carriage and possible infection. © Springer Science+Business Media B.V. 2009.
Persistent Identifierhttp://hdl.handle.net/10722/154590
ISSN
2015 Impact Factor: 1.671
2015 SCImago Journal Rankings: 0.720
ISI Accession Number ID
Funding AgencyGrant Number
Pontifical Catholic University of Parana (PUCPR)
University of Hong Kong (HKU)
National Council of Technological and Scientific Development (CNPq)
Funding Information:

This study was conducted using intramural funds from The Pontifical Catholic University of Parana (PUCPR) and The University of Hong Kong (HKU). It was part of the Scientific Initiation Program of F.B.B. that was granted by the National Council of Technological and Scientific Development (CNPq). D.B. is a research fellow supported by the Scientific Initiation Program of PUCPR.

References

 

DC FieldValueLanguage
dc.contributor.authorBaboni, FBen_US
dc.contributor.authorBarp, Den_US
dc.contributor.authorDe Azevedo Izidoro, ACSen_US
dc.contributor.authorSamaranayake, LPen_US
dc.contributor.authorRosa, EARen_US
dc.date.accessioned2012-08-08T08:26:22Z-
dc.date.available2012-08-08T08:26:22Z-
dc.date.issued2009en_US
dc.identifier.citationMycopathologia, 2009, v. 168 n. 5, p. 227-235en_US
dc.identifier.issn0301-486Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/154590-
dc.description.abstractThe habit of cigarette smoking is associated with higher oral candidal carriage and possible predisposition to oral candidosis. The effects of exposure to smoke on the virulence properties of oral yeasts remain obscure. Hence, we showed in vitro the effect of cigarette smoke condensate (CSC) on ten clinical isolates of Candida albicans obtained from nonsmoking volunteers, as well the type-strain CBS562. CSC was generated by complete burn of five commercial cigarettes in an in-house smoking machine and used to prepare the culture broth in which the strains were grown. In 24-h intervals (T24, T48, and T72), the cells were harvested, washed, subcultured, and the resultant growth were evaluated for possible variations for secreted aspartyl protease, phospholipase, chondroitinase, and hemolysins, adhesion to acrylic and cell surface hydrophobicity (CSH). The results indicated a temporal increase in the secretion rates of enzymes, particularly when yeast cells were exposed to CSC for 48-72h (P < 0.05). Similarly, adhesion to acrylic and CSH increased with exposure period (P < 0.05). Based on foregoing, we concluded that CSC may promote significant enhance in the secretion of candidal histolytic enzymes and adherence to denture surfaces, thereby promoting oral yeast carriage and possible infection. © Springer Science+Business Media B.V. 2009.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0301-486Xen_US
dc.relation.ispartofMycopathologiaen_US
dc.subject.meshAspartic Acid Proteases - Metabolismen_US
dc.subject.meshCandida Albicans - Drug Effects - Enzymology - Growth & Development - Metabolism - Pathogenicityen_US
dc.subject.meshCandidiasis, Oral - Microbiologyen_US
dc.subject.meshChondroitinases And Chondroitin Lyases - Metabolismen_US
dc.subject.meshHemolysin Proteins - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshHydrophobic And Hydrophilic Interactionsen_US
dc.subject.meshPhospholipases - Metabolismen_US
dc.subject.meshSmokeen_US
dc.subject.meshSmokingen_US
dc.subject.meshVirulence - Drug Effectsen_US
dc.titleEnhancement of Candida albicans virulence after exposition to cigarette mainstream smokeen_US
dc.typeArticleen_US
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s11046-009-9217-5en_US
dc.identifier.pmid19544010-
dc.identifier.scopuseid_2-s2.0-70350370772en_US
dc.identifier.hkuros166623-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70350370772&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume168en_US
dc.identifier.issue5en_US
dc.identifier.spage227en_US
dc.identifier.epage235en_US
dc.identifier.isiWOS:000270742500002-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridBaboni, FB=35104430300en_US
dc.identifier.scopusauthoridBarp, D=35309256600en_US
dc.identifier.scopusauthoridde Azevedo Izidoro, ACS=23472880600en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US
dc.identifier.scopusauthoridRosa, EAR=7004952748en_US
dc.identifier.citeulike4940507-

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