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Article: Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor during healing of allogeneic bone graft

TitleBasic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor during healing of allogeneic bone graft
Authors
KeywordsAllogeneic bone graft
bFGF
DBM IM
Revascularization
VEGF
Issue Date2004
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
Citation
Archives Of Oral Biology, 2004, v. 49 n. 12, p. 1025-1033 How to Cite?
AbstractRecently we reported that basic fibroblast growth factor (bFGF) improved the healing of allogeneic bone grafts. However, the mechanism of action of the bFGF was not known. Therefore, the present study was designed to identify the expression pattern of vascular endothelial growth factor (VEGF) in the presence of bFGF reconstituted in demineralized intramembranous bone matrix (DBM IM) during the healing of allogeneic bone grafts. Eighteen critical size (15 mm × 10 mm) defects were created on rabbit mandibles bilaterally. Three groups of six defects each were grafted with allogeneic bone alone, allogeneic bone and DBM IM, and allogeneic bone and bFGF reconstituted in DBM IM. Three weeks later, the defects were retrieved for immunohistochemistry and in situ hybridization for VEGF. The percentage of positive staining area was quantified by using image analyzer. The increase (517%) in the expression of VEGF mRNA was accompanied by an increase (492%) of immunoreactive VEGF protein in allogeneic bone graft augmented by bFGF reconstituted in DBM IM. A close correlation existed between levels of VEGF production and the amount of newly formed bone. The results show that bFGF reconstituted in DBM IM markedly up-regulated the expression of VEGF in the grafted area. Basic FGF augments the healing of allogeneic bone grafts by enhancing vascularization through the up-regulation of VEGF. © 2004 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/154550
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.713
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorRabie, ABMen_HK
dc.contributor.authorLu, Men_HK
dc.date.accessioned2012-08-08T08:26:07Z-
dc.date.available2012-08-08T08:26:07Z-
dc.date.issued2004en_HK
dc.identifier.citationArchives Of Oral Biology, 2004, v. 49 n. 12, p. 1025-1033en_HK
dc.identifier.issn0003-9969en_HK
dc.identifier.urihttp://hdl.handle.net/10722/154550-
dc.description.abstractRecently we reported that basic fibroblast growth factor (bFGF) improved the healing of allogeneic bone grafts. However, the mechanism of action of the bFGF was not known. Therefore, the present study was designed to identify the expression pattern of vascular endothelial growth factor (VEGF) in the presence of bFGF reconstituted in demineralized intramembranous bone matrix (DBM IM) during the healing of allogeneic bone grafts. Eighteen critical size (15 mm × 10 mm) defects were created on rabbit mandibles bilaterally. Three groups of six defects each were grafted with allogeneic bone alone, allogeneic bone and DBM IM, and allogeneic bone and bFGF reconstituted in DBM IM. Three weeks later, the defects were retrieved for immunohistochemistry and in situ hybridization for VEGF. The percentage of positive staining area was quantified by using image analyzer. The increase (517%) in the expression of VEGF mRNA was accompanied by an increase (492%) of immunoreactive VEGF protein in allogeneic bone graft augmented by bFGF reconstituted in DBM IM. A close correlation existed between levels of VEGF production and the amount of newly formed bone. The results show that bFGF reconstituted in DBM IM markedly up-regulated the expression of VEGF in the grafted area. Basic FGF augments the healing of allogeneic bone grafts by enhancing vascularization through the up-regulation of VEGF. © 2004 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbioen_HK
dc.relation.ispartofArchives of Oral Biologyen_HK
dc.subjectAllogeneic bone graften_HK
dc.subjectbFGFen_HK
dc.subjectDBM IMen_HK
dc.subjectRevascularizationen_HK
dc.subjectVEGFen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshBone Transplantation - Physiologyen_US
dc.subject.meshBone And Bones - Anatomy & Histology - Physiologyen_US
dc.subject.meshFibroblast Growth Factor 2 - Physiologyen_US
dc.subject.meshImmunohistochemistry - Methodsen_US
dc.subject.meshIn Situ Hybridization - Methodsen_US
dc.subject.meshNeovascularization, Physiologic - Physiologyen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshRabbitsen_US
dc.subject.meshUp-Regulation - Physiologyen_US
dc.subject.meshVascular Endothelial Growth Factors - Physiologyen_US
dc.subject.meshWound Healing - Physiologyen_US
dc.titleBasic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor during healing of allogeneic bone graften_HK
dc.typeArticleen_HK
dc.identifier.emailRabie, ABM: rabie@hku.hken_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.archoralbio.2004.05.012en_HK
dc.identifier.pmid15485645-
dc.identifier.scopuseid_2-s2.0-5644227666en_HK
dc.identifier.hkuros98722-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-5644227666&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume49en_HK
dc.identifier.issue12en_HK
dc.identifier.spage1025en_HK
dc.identifier.epage1033en_HK
dc.identifier.isiWOS:000225233900010-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridRabie, ABM=7007172734en_HK
dc.identifier.scopusauthoridLu, M=7402821078en_HK

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