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Article: In vitro growth, acidogenicity and cariogenicity of predominant human root caries flora

TitleIn vitro growth, acidogenicity and cariogenicity of predominant human root caries flora
Authors
KeywordsCariogenicity and artificial caries
Microbiology
Root caries
Issue Date2004
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent
Citation
Journal Of Dentistry, 2004, v. 32 n. 8, p. 667-678 How to Cite?
AbstractStreptococcus mutans (Sm), Lactobacillus acidophilus (La) and Actinomyces israelii (Ai) have been associated with root surface caries, which is an increasing problem in elderly Chinese. The aim of this study therefore, was to evaluate in vitro, the growth, acidogenicity and cariogenicity of these organisms, both in mono- and co-cultures using an in vitro model. Forty-eight root specimens were prepared using intact extracted human molars. Fresh, wild-type bacteria obtained from root caries lesions were assembled into seven experimental groups as either mono- or co-cultures and incubated with the root specimens. Appropriate controls were included. Growth curve of each experimental group was monitored for 24 h, aerobically, at 37°C using a microplate reader. The pH of the medium was recorded after 24-h incubation using a pH meter. Mean depths of artificial root lesions produced in each cultural group were measured using polarized light microscopy in specimens cut into thin sections (100±20 μm). Compared with mono-cultures, synergistic growth was observed in co-cultures of 'La+Sm', 'Ai+La' and 'Ai+La+Sm'. Mean lesion depth produced in La group was significantly shallower than other mono- or co-culture groups (p<0.01). The pH values of all culture media were similar after 24-h incubation. The current data elucidate the complex interactions of three predominant bacterial species considered prime agents of human root surface caries. © 2004 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/154542
ISSN
2015 Impact Factor: 3.109
2015 SCImago Journal Rankings: 1.029
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShen, Sen_US
dc.contributor.authorSamaranayake, LPen_US
dc.contributor.authorYip, HKen_US
dc.date.accessioned2012-08-08T08:26:04Z-
dc.date.available2012-08-08T08:26:04Z-
dc.date.issued2004en_US
dc.identifier.citationJournal Of Dentistry, 2004, v. 32 n. 8, p. 667-678en_US
dc.identifier.issn0300-5712en_US
dc.identifier.urihttp://hdl.handle.net/10722/154542-
dc.description.abstractStreptococcus mutans (Sm), Lactobacillus acidophilus (La) and Actinomyces israelii (Ai) have been associated with root surface caries, which is an increasing problem in elderly Chinese. The aim of this study therefore, was to evaluate in vitro, the growth, acidogenicity and cariogenicity of these organisms, both in mono- and co-cultures using an in vitro model. Forty-eight root specimens were prepared using intact extracted human molars. Fresh, wild-type bacteria obtained from root caries lesions were assembled into seven experimental groups as either mono- or co-cultures and incubated with the root specimens. Appropriate controls were included. Growth curve of each experimental group was monitored for 24 h, aerobically, at 37°C using a microplate reader. The pH of the medium was recorded after 24-h incubation using a pH meter. Mean depths of artificial root lesions produced in each cultural group were measured using polarized light microscopy in specimens cut into thin sections (100±20 μm). Compared with mono-cultures, synergistic growth was observed in co-cultures of 'La+Sm', 'Ai+La' and 'Ai+La+Sm'. Mean lesion depth produced in La group was significantly shallower than other mono- or co-culture groups (p<0.01). The pH values of all culture media were similar after 24-h incubation. The current data elucidate the complex interactions of three predominant bacterial species considered prime agents of human root surface caries. © 2004 Elsevier Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdenten_US
dc.relation.ispartofJournal of Dentistryen_US
dc.rightsJournal of Dentistry. Copyright © Elsevier Ltd.-
dc.subjectCariogenicity and artificial caries-
dc.subjectMicrobiology-
dc.subjectRoot caries-
dc.subject.meshAcids - Metabolismen_US
dc.subject.meshActinomyces - Growth & Development - Metabolism - Pathogenicityen_US
dc.subject.meshColony Count, Microbialen_US
dc.subject.meshDental Caries Activity Testsen_US
dc.subject.meshGram-Positive Bacteria - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshLactobacillus - Growth & Development - Metabolism - Pathogenicityen_US
dc.subject.meshMolar - Microbiology - Pathologyen_US
dc.subject.meshRoot Caries - Microbiology - Pathologyen_US
dc.subject.meshStreptococcus Mutans - Growth & Development - Metabolism - Pathogenicityen_US
dc.subject.meshTooth Demineralization - Microbiologyen_US
dc.subject.meshTooth Root - Microbiology - Pathologyen_US
dc.titleIn vitro growth, acidogenicity and cariogenicity of predominant human root caries floraen_US
dc.typeArticleen_US
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_US
dc.identifier.emailYip, HK:kevin.h.k.yip@hkusua.hku.hken_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.identifier.authorityYip, HK=rp00027en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jdent.2004.07.002en_US
dc.identifier.pmid15476962-
dc.identifier.scopuseid_2-s2.0-5144220396en_US
dc.identifier.hkuros96480-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-5144220396&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume32en_US
dc.identifier.issue8en_US
dc.identifier.spage667en_US
dc.identifier.epage678en_US
dc.identifier.isiWOS:000224670100010-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridShen, S=7403431743en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US
dc.identifier.scopusauthoridYip, HK=25423244900en_US

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