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Article: A comparative study of candidal invasion in rabbit tongue mucosal explants and reconstituted human oral epithelium

TitleA comparative study of candidal invasion in rabbit tongue mucosal explants and reconstituted human oral epithelium
Authors
Issue Date2008
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0301-486X
Citation
Mycopathologia, 2008, v. 165 n. 6, p. 373-380 How to Cite?
AbstractThe purpose of this study is to compare the light and scanning electron microscopic (SEM) features of tissue invasion by three Candida species (C. albicans, C. tropicalis, and C. dubliniensis) in two different tissue culture models: rabbit tongue mucosal explants (RTME) and reconstituted human oral epithelium (RHOE). Tongue mucosal biopsies of healthy New Zealand rabbits were maintained in explant culture using a transwell system. RHOE was obtained from Skinethic Laboratory (Nice, France). RTME and RHOE were inoculated with C. albicans, C. tropicalis, and C. dubliniensis separately and incubated at 37°C, 5% CO2, and 100% humidity up to 48 h. Light microscopic and SEM examinations of uninfected (controls) and infected tissues were performed at 24 and 48 h. C. albicans produced characteristic hallmarks of pathological tissue invasion in both tissue models over a period of 48 h. Hyphae penetrated through epithelial cells and intercellular gaps latter resembling thigmotropism. SEM showed cavitations on the epithelial cell surfaces particularly pronounced at sites of hyphal invasion. Some hyphae on RTME showed several clusters of blastospores attached in regular arrangements resembling "appareil sporifere". C. tropicalis and C. dubliniensis produced few hyphae mainly on RTME but they did not penetrate either model. Our findings indicate that multiple host-fungal interactions such as cavitations, thigmotropism, and morphogenesis take place during candidal tissue invasion. RTME described here appears to be useful in investigations of such pathogenic processes of Candida active at the epithelial front. © 2008 Springer Science+Business Media B.V.
Persistent Identifierhttp://hdl.handle.net/10722/154522
ISSN
2015 Impact Factor: 1.671
2015 SCImago Journal Rankings: 0.720
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJayatilake, JAMSen_US
dc.contributor.authorSamaranayake, YHen_US
dc.contributor.authorSamaranayake, LPen_US
dc.date.accessioned2012-08-08T08:25:57Z-
dc.date.available2012-08-08T08:25:57Z-
dc.date.issued2008en_US
dc.identifier.citationMycopathologia, 2008, v. 165 n. 6, p. 373-380en_US
dc.identifier.issn0301-486Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/154522-
dc.description.abstractThe purpose of this study is to compare the light and scanning electron microscopic (SEM) features of tissue invasion by three Candida species (C. albicans, C. tropicalis, and C. dubliniensis) in two different tissue culture models: rabbit tongue mucosal explants (RTME) and reconstituted human oral epithelium (RHOE). Tongue mucosal biopsies of healthy New Zealand rabbits were maintained in explant culture using a transwell system. RHOE was obtained from Skinethic Laboratory (Nice, France). RTME and RHOE were inoculated with C. albicans, C. tropicalis, and C. dubliniensis separately and incubated at 37°C, 5% CO2, and 100% humidity up to 48 h. Light microscopic and SEM examinations of uninfected (controls) and infected tissues were performed at 24 and 48 h. C. albicans produced characteristic hallmarks of pathological tissue invasion in both tissue models over a period of 48 h. Hyphae penetrated through epithelial cells and intercellular gaps latter resembling thigmotropism. SEM showed cavitations on the epithelial cell surfaces particularly pronounced at sites of hyphal invasion. Some hyphae on RTME showed several clusters of blastospores attached in regular arrangements resembling "appareil sporifere". C. tropicalis and C. dubliniensis produced few hyphae mainly on RTME but they did not penetrate either model. Our findings indicate that multiple host-fungal interactions such as cavitations, thigmotropism, and morphogenesis take place during candidal tissue invasion. RTME described here appears to be useful in investigations of such pathogenic processes of Candida active at the epithelial front. © 2008 Springer Science+Business Media B.V.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0301-486Xen_US
dc.relation.ispartofMycopathologiaen_US
dc.rightsThe original publication is available at www.springerlink.com-
dc.subject.meshAnimalsen_US
dc.subject.meshCandida - Genetics - Pathogenicityen_US
dc.subject.meshCandidiasis, Oral - Microbiology - Pathologyen_US
dc.subject.meshEpithelial Cells - Microbiologyen_US
dc.subject.meshEpithelium - Microbiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMicroscopy, Electron, Scanningen_US
dc.subject.meshMouth Mucosa - Microbiologyen_US
dc.subject.meshRabbitsen_US
dc.subject.meshTissue Culture Techniquesen_US
dc.subject.meshTongueen_US
dc.titleA comparative study of candidal invasion in rabbit tongue mucosal explants and reconstituted human oral epitheliumen_US
dc.typeArticleen_US
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0301-486X&volume=165&issue=6&spage=373&epage=80&date=2008&atitle=A+comparative+studyof+candidal+invasion+in+rabbit+tongue+mucosal+explants+and+reconstituted+human+oral+epithelium-
dc.identifier.emailJayatilake, JAMS: sumedha@hkusua.hku.hken_US
dc.identifier.emailSamaranayake, YH:hema@hkucc.hku.hken_US
dc.identifier.emailSamaranayake, LP:lakshman@hku.hk-
dc.identifier.authoritySamaranayake, YH=rp00025en_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s11046-008-9096-1en_US
dc.identifier.pmid18320346-
dc.identifier.scopuseid_2-s2.0-44449170264en_US
dc.identifier.hkuros142145-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-44449170264&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume165en_US
dc.identifier.issue6en_US
dc.identifier.spage373en_US
dc.identifier.epage380en_US
dc.identifier.isiWOS:000256091200003-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridJayatilake, JAMS=8441671500en_US
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US

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