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Article: Factors regulating endochondral ossification in the sphenooccipital synchondrosis

TitleFactors regulating endochondral ossification in the sphenooccipital synchondrosis
Authors
KeywordsCbfa 1
Endochondral ossification
Organ culture
Spheno-occipital synchondrosis
VEGF
Issue Date2008
PublisherE H Angle Education and Research Foundation, Inc. The Journal's web site is located at http://www.angle.org/
Citation
Angle Orthodontist, 2008, v. 78 n. 2, p. 215-220 How to Cite?
AbstractObjectives: To identify the temporal pattern of core-binding factor a1 (Cbfa1) and vascular endothelial growth factor (VEGF) expressions in the spheno-occipital synchondrosis in vitro with and without tensile stress. Materials and Methods: Sixty male BALB/c mice were randomly divided into an experimental group (with tensile stress) and a control group (without tensile stress) at each of five time points. Animals were sacrificed and the cranial base synchondroses were aseptically removed. In the experimental groups, mechanical stress was applied on the surgical explants with helical springs and incubated as organ culture for 6, 24, 48, 72, and 168 hours. In the control group, the springs were kept at zero stress. Tissue sections were subjected to immunohistochemical staining for quantitative analysis of Cbfa1 and VEGF expression. Results: Quantitative analysis revealed that Cbfa1 and VEGF expressions reached a peak increase at 24 and 48 hours, respectively. Compared with the control groups, both Cbfa1 and VEGF were expressed consistently higher in the experimental groups at all time points. Conclusion: Mechanical stress applied to the spheno-occipital synchondrosis elicits Cbfa1 expression and subsequently up-regulates the expression of VEGF. Increased levels of expression of both factors could play a role in the growth of the spheno-occipital synchondrosis. © 2007 by The EH Angle Education and Research Foundation, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/154506
ISSN
2015 Impact Factor: 1.579
2015 SCImago Journal Rankings: 1.254
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLei, WYen_HK
dc.contributor.authorWong, RWKen_HK
dc.contributor.authorRabie, ABMen_HK
dc.date.accessioned2012-08-08T08:25:51Z-
dc.date.available2012-08-08T08:25:51Z-
dc.date.issued2008en_HK
dc.identifier.citationAngle Orthodontist, 2008, v. 78 n. 2, p. 215-220en_HK
dc.identifier.issn0003-3219en_HK
dc.identifier.urihttp://hdl.handle.net/10722/154506-
dc.description.abstractObjectives: To identify the temporal pattern of core-binding factor a1 (Cbfa1) and vascular endothelial growth factor (VEGF) expressions in the spheno-occipital synchondrosis in vitro with and without tensile stress. Materials and Methods: Sixty male BALB/c mice were randomly divided into an experimental group (with tensile stress) and a control group (without tensile stress) at each of five time points. Animals were sacrificed and the cranial base synchondroses were aseptically removed. In the experimental groups, mechanical stress was applied on the surgical explants with helical springs and incubated as organ culture for 6, 24, 48, 72, and 168 hours. In the control group, the springs were kept at zero stress. Tissue sections were subjected to immunohistochemical staining for quantitative analysis of Cbfa1 and VEGF expression. Results: Quantitative analysis revealed that Cbfa1 and VEGF expressions reached a peak increase at 24 and 48 hours, respectively. Compared with the control groups, both Cbfa1 and VEGF were expressed consistently higher in the experimental groups at all time points. Conclusion: Mechanical stress applied to the spheno-occipital synchondrosis elicits Cbfa1 expression and subsequently up-regulates the expression of VEGF. Increased levels of expression of both factors could play a role in the growth of the spheno-occipital synchondrosis. © 2007 by The EH Angle Education and Research Foundation, Inc.en_HK
dc.languageengen_US
dc.publisherE H Angle Education and Research Foundation, Inc. The Journal's web site is located at http://www.angle.org/en_HK
dc.relation.ispartofAngle Orthodontisten_HK
dc.subjectCbfa 1en_HK
dc.subjectEndochondral ossificationen_HK
dc.subjectOrgan cultureen_HK
dc.subjectSpheno-occipital synchondrosisen_HK
dc.subjectVEGFen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshChondrocytes - Metabolismen_US
dc.subject.meshChondrogenesis - Physiologyen_US
dc.subject.meshCore Binding Factor Alpha 1 Subunit - Biosynthesisen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Balb Cen_US
dc.subject.meshOccipital Bone - Growth & Developmenten_US
dc.subject.meshOrgan Culture Techniquesen_US
dc.subject.meshOsteoblasts - Metabolismen_US
dc.subject.meshOsteogenesis - Physiologyen_US
dc.subject.meshSkull Base - Growth & Developmenten_US
dc.subject.meshSphenoid Bone - Growth & Developmenten_US
dc.subject.meshStress, Mechanicalen_US
dc.subject.meshTensile Strengthen_US
dc.subject.meshUp-Regulationen_US
dc.subject.meshVascular Endothelial Growth Factor A - Biosynthesisen_US
dc.titleFactors regulating endochondral ossification in the sphenooccipital synchondrosisen_HK
dc.typeArticleen_HK
dc.identifier.emailWong, RWK: fyoung@hku.hken_HK
dc.identifier.emailRabie, ABM: rabie@hku.hken_HK
dc.identifier.authorityWong, RWK=rp00038en_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.2319/020707-59.1en_HK
dc.identifier.pmid18251599-
dc.identifier.scopuseid_2-s2.0-40749108459en_HK
dc.identifier.hkuros141203-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-40749108459&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume78en_HK
dc.identifier.issue2en_HK
dc.identifier.spage215en_HK
dc.identifier.epage220en_HK
dc.identifier.isiWOS:000253238800005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLei, WY=23975987500en_HK
dc.identifier.scopusauthoridWong, RWK=7402127170en_HK
dc.identifier.scopusauthoridRabie, ABM=7007172734en_HK

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