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Article: Noncanonical Wnt-4 signaling enhances bone regeneration of mesenchymal stem cells in craniofacial defects through activation of p38 MAPK

TitleNoncanonical Wnt-4 signaling enhances bone regeneration of mesenchymal stem cells in craniofacial defects through activation of p38 MAPK
Authors
Issue Date2007
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 2007, v. 282 n. 42, p. 30938-30948 How to Cite?
AbstractMesenchymal stem cells (MSCs) are multipotent cells that can be differentiated into osteoblasts and provide an excellent cell source for bone regeneration and repair. Recently, the canonical Wnt/β-catenin signaling pathway has been found to play a critical role in skeletal development and osteogenesis, implying that Wnts can be utilized to improve de novo bone formation mediated by MSCs. However, it is unknown whether noncanonical Wnt signaling regulates osteogenic differentiation. Here, we find that Wnt-4 enhanced in vitro osteogenic differentiation of MSCs isolated from human adult craniofacial tissues and promoted bone formation in vivo. Whereas Wnt-4 did not stabilize β-catenin, it activated p38 MAPK in a novel noncanonical signaling pathway. The activation of p38 was dependent on Axin and was required for the enhancement of MSC differentiation by Wnt-4. Moreover, using two different models of craniofacial bone injury, we found that MSCs genetically engineered to express Wnt-4 enhanced osteogenesis and improved the repair of craniofacial defects in vivo. Taken together, our results reveal that noncanonical Wnt signaling could also play a role in osteogenic differentiation. Wnt-4 may have a potential use in improving bone regeneration and repair of craniofacial defects. © 2007 by The American Society for Biochemistry and Molecular Biology, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/154494
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChang, Jen_US
dc.contributor.authorSonoyama, Wen_US
dc.contributor.authorWang, Zen_US
dc.contributor.authorJin, Qen_US
dc.contributor.authorZhang, Cen_US
dc.contributor.authorKrebsbach, PHen_US
dc.contributor.authorGiannobile, Wen_US
dc.contributor.authorShi, Sen_US
dc.contributor.authorWang, CYen_US
dc.date.accessioned2012-08-08T08:25:39Z-
dc.date.available2012-08-08T08:25:39Z-
dc.date.issued2007en_US
dc.identifier.citationJournal Of Biological Chemistry, 2007, v. 282 n. 42, p. 30938-30948en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/154494-
dc.description.abstractMesenchymal stem cells (MSCs) are multipotent cells that can be differentiated into osteoblasts and provide an excellent cell source for bone regeneration and repair. Recently, the canonical Wnt/β-catenin signaling pathway has been found to play a critical role in skeletal development and osteogenesis, implying that Wnts can be utilized to improve de novo bone formation mediated by MSCs. However, it is unknown whether noncanonical Wnt signaling regulates osteogenic differentiation. Here, we find that Wnt-4 enhanced in vitro osteogenic differentiation of MSCs isolated from human adult craniofacial tissues and promoted bone formation in vivo. Whereas Wnt-4 did not stabilize β-catenin, it activated p38 MAPK in a novel noncanonical signaling pathway. The activation of p38 was dependent on Axin and was required for the enhancement of MSC differentiation by Wnt-4. Moreover, using two different models of craniofacial bone injury, we found that MSCs genetically engineered to express Wnt-4 enhanced osteogenesis and improved the repair of craniofacial defects in vivo. Taken together, our results reveal that noncanonical Wnt signaling could also play a role in osteogenic differentiation. Wnt-4 may have a potential use in improving bone regeneration and repair of craniofacial defects. © 2007 by The American Society for Biochemistry and Molecular Biology, Inc.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAxin Proteinen_US
dc.subject.meshBone Regenerationen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCraniofacial Abnormalities - Metabolism - Pathologyen_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshEnzyme Activationen_US
dc.subject.meshFacial Bones - Injuries - Metabolism - Pathologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshMap Kinase Signaling Systemen_US
dc.subject.meshMesenchymal Stem Cells - Enzymology - Pathologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Sciden_US
dc.subject.meshMultipotent Stem Cells - Enzymology - Pathologyen_US
dc.subject.meshOsteogenesisen_US
dc.subject.meshRepressor Proteins - Metabolismen_US
dc.subject.meshWnt Proteins - Metabolismen_US
dc.subject.meshWnt4 Proteinen_US
dc.subject.meshBeta Catenin - Metabolismen_US
dc.subject.meshP38 Mitogen-Activated Protein Kinases - Metabolismen_US
dc.titleNoncanonical Wnt-4 signaling enhances bone regeneration of mesenchymal stem cells in craniofacial defects through activation of p38 MAPKen_US
dc.typeArticleen_US
dc.identifier.emailZhang, C:zhangcf@hku.hken_US
dc.identifier.authorityZhang, C=rp01408en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1074/jbc.M702391200en_US
dc.identifier.pmid17720811en_US
dc.identifier.scopuseid_2-s2.0-35648990464en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-35648990464&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume282en_US
dc.identifier.issue42en_US
dc.identifier.spage30938en_US
dc.identifier.epage30948en_US
dc.identifier.isiWOS:000250136300062-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChang, J=12762048800en_US
dc.identifier.scopusauthoridSonoyama, W=12447072100en_US
dc.identifier.scopusauthoridWang, Z=7410041989en_US
dc.identifier.scopusauthoridJin, Q=7202528058en_US
dc.identifier.scopusauthoridZhang, C=7405494609en_US
dc.identifier.scopusauthoridKrebsbach, PH=7004077362en_US
dc.identifier.scopusauthoridGiannobile, W=7004619325en_US
dc.identifier.scopusauthoridShi, S=7402200823en_US
dc.identifier.scopusauthoridWang, CY=35276383300en_US
dc.identifier.citeulike2954461-

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