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Article: IL-1α, IL-1ra and IL-8 are differentially induced by Candida in experimental oral candidiasis

TitleIL-1α, IL-1ra and IL-8 are differentially induced by Candida in experimental oral candidiasis
Authors
Issue Date2007
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1
Citation
Oral Diseases, 2007, v. 13 n. 4, p. 426-433 How to Cite?
AbstractObjective: To investigate the expression of interleukin-1α (IL-1α), IL-1ra and IL-8 by the oral epithelium challenged by various Candida species. Materials and methods: In vitro candidiasis was induced by C. albicans wild type SC5314, its EFG1, CPH1 and secretory aspartyl proteinase (SAP) mutants and, ATCC isolates of C. albicans, C. tropicalis and C. dubliniensis using a reconstituted human oral epithelium (RHOE) model. IL-1α, IL-1ra and IL-8 levels in culture media were quantified by an enzyme-linked immunosorbent assay at 12, 24 and 48 h. Fungal invasion and IL-1ra expression in RHOE were detected by periodic acid-Schiff staining and immunohistochemistry. Results: Overall, the invasive Candida induced relatively higher levels of IL-1α, IL-1ra and IL-8 in the culture media than the noninvasive isolates. IL-1α and IL-1ra levels induced by Candida with hyphal invasion were significantly higher (P < 0.05) than those induced by the isolates without hyphal invasion at 12, 24 and 48 h. Candida albicans SC5314 induced IL-1ra expression in RHOE at 12 and 24 h but not at 48 h consistent with its hyphal invasion; while the noninvasive mutants and non-albicans Candida induced IL-1ra expression at 48 h. Conclusions: The cytokine expression profiles in experimental oral candidiasis may be associated with the invasive potential of Candida. © 2007 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/154465
ISSN
2015 Impact Factor: 2.0
2015 SCImago Journal Rankings: 0.828
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJayatilake, JAMSen_US
dc.contributor.authorSamaranayake, LPen_US
dc.contributor.authorLu, Qen_US
dc.contributor.authorJin, LJen_US
dc.date.accessioned2012-08-08T08:25:28Z-
dc.date.available2012-08-08T08:25:28Z-
dc.date.issued2007en_US
dc.identifier.citationOral Diseases, 2007, v. 13 n. 4, p. 426-433en_US
dc.identifier.issn1354-523Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/154465-
dc.description.abstractObjective: To investigate the expression of interleukin-1α (IL-1α), IL-1ra and IL-8 by the oral epithelium challenged by various Candida species. Materials and methods: In vitro candidiasis was induced by C. albicans wild type SC5314, its EFG1, CPH1 and secretory aspartyl proteinase (SAP) mutants and, ATCC isolates of C. albicans, C. tropicalis and C. dubliniensis using a reconstituted human oral epithelium (RHOE) model. IL-1α, IL-1ra and IL-8 levels in culture media were quantified by an enzyme-linked immunosorbent assay at 12, 24 and 48 h. Fungal invasion and IL-1ra expression in RHOE were detected by periodic acid-Schiff staining and immunohistochemistry. Results: Overall, the invasive Candida induced relatively higher levels of IL-1α, IL-1ra and IL-8 in the culture media than the noninvasive isolates. IL-1α and IL-1ra levels induced by Candida with hyphal invasion were significantly higher (P < 0.05) than those induced by the isolates without hyphal invasion at 12, 24 and 48 h. Candida albicans SC5314 induced IL-1ra expression in RHOE at 12 and 24 h but not at 48 h consistent with its hyphal invasion; while the noninvasive mutants and non-albicans Candida induced IL-1ra expression at 48 h. Conclusions: The cytokine expression profiles in experimental oral candidiasis may be associated with the invasive potential of Candida. © 2007 The Authors.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1en_US
dc.relation.ispartofOral Diseasesen_US
dc.subject.meshCandida - Classification - Genetics - Immunologyen_US
dc.subject.meshCandida Albicans - Immunologyen_US
dc.subject.meshCandida Tropicalis - Immunologyen_US
dc.subject.meshCandidiasis, Oral - Immunology - Pathologyen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshEpithelium - Immunology - Microbiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshHyphae - Physiology - Ultrastructureen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshInterleukin-1Alpha - Analysisen_US
dc.subject.meshInterleukin-8 - Analysisen_US
dc.subject.meshMouth Mucosa - Cytology - Immunology - Microbiologyen_US
dc.subject.meshMutation - Geneticsen_US
dc.subject.meshReceptors, Interleukin-1 Type I - Analysisen_US
dc.subject.meshTime Factorsen_US
dc.titleIL-1α, IL-1ra and IL-8 are differentially induced by Candida in experimental oral candidiasisen_US
dc.typeArticleen_US
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_US
dc.identifier.emailJin, LJ:ljjin@hkucc.hku.hken_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.identifier.authorityJin, LJ=rp00028en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1601-0825.2007.01318.xen_US
dc.identifier.pmid17577331en_US
dc.identifier.scopuseid_2-s2.0-34250650368en_US
dc.identifier.hkuros128375-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34250650368&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume13en_US
dc.identifier.issue4en_US
dc.identifier.spage426en_US
dc.identifier.epage433en_US
dc.identifier.isiWOS:000247978100012-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridJayatilake, JAMS=8441671500en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US
dc.identifier.scopusauthoridLu, Q=36128876000en_US
dc.identifier.scopusauthoridJin, LJ=7403328850en_US
dc.identifier.citeulike1396348-

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