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Article: A dominant function of IKK/NF-κB signaling in global lipopolysaccharide-induced gene expression
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TitleA dominant function of IKK/NF-κB signaling in global lipopolysaccharide-induced gene expression
 
AuthorsCarayol, N3
Chen, J2
Yang, F3
Jin, T3
Jin, L1
States, D2
Wang, CY3
 
Issue Date2006
 
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
 
CitationJournal Of Biological Chemistry, 2006, v. 281 n. 41, p. 31142-31151 [How to Cite?]
DOI: http://dx.doi.org/10.1074/jbc.M603417200
 
AbstractPorphyromonas gingivalis is an etiologic pathogen of periodontitis that is one of the most common inflammatory diseases. Recently, we found that P. gingivalis LPS activated the transcription factor nuclear factor-κB (NF-κB) through the IκB kinase complex (IKK). NF-κB is a transcription factor that controls inflammation and host responses. In this study, we examined the role of IKK/NF-κB in P. gingivalis LPS-induced gene expression on a genome-wide basis using a combination of microarray and biochemical approaches. A total of 88 early response genes were found to be induced by P. gingivalis LPS in a human THP.1 monocytic cell lines. Interestingly, the induction of most of these genes was abolished or attenuated under the inactivation of IKK/NF-κB. Among those IKK/NF-κB-dependent genes, 20 genes were NF-κB-inducible genes reported previously, and 59 genes represented putative novel NF-κB target genes. Using transcription factor binding analysis, we found that most of these putative NF-κB target genes contained one or multiple NF-κB-binding sites. Also, some transcription factor-binding motifs were overrepresented in the promoter of both known and putative NF-κB-dependent genes, indicating that these genes may be regulated in a similar fashion. Furthermore, we found that several transcription factors associated with metabolic and inflammatory responses, including nuclear receptors, activator of protein-1, and early growth responses, were induced by P. gingivalis LPS through IKK/NF-κB, indicating that IKK/NF-κB may utilize these transcription factors to mediate secondary responses. Taken together, our results demonstrate that IKK/NF-κB signaling plays a dominant role in P. gingivalis LPS-induced early response gene expression, suggesting that IKK/NF-κB is a therapeutic target for periodontitis. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc.
 
ISSN0021-9258
2013 Impact Factor: 4.600
 
DOIhttp://dx.doi.org/10.1074/jbc.M603417200
 
ISI Accession Number IDWOS:000241075900089
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorCarayol, N
 
dc.contributor.authorChen, J
 
dc.contributor.authorYang, F
 
dc.contributor.authorJin, T
 
dc.contributor.authorJin, L
 
dc.contributor.authorStates, D
 
dc.contributor.authorWang, CY
 
dc.date.accessioned2012-08-08T08:25:16Z
 
dc.date.available2012-08-08T08:25:16Z
 
dc.date.issued2006
 
dc.description.abstractPorphyromonas gingivalis is an etiologic pathogen of periodontitis that is one of the most common inflammatory diseases. Recently, we found that P. gingivalis LPS activated the transcription factor nuclear factor-κB (NF-κB) through the IκB kinase complex (IKK). NF-κB is a transcription factor that controls inflammation and host responses. In this study, we examined the role of IKK/NF-κB in P. gingivalis LPS-induced gene expression on a genome-wide basis using a combination of microarray and biochemical approaches. A total of 88 early response genes were found to be induced by P. gingivalis LPS in a human THP.1 monocytic cell lines. Interestingly, the induction of most of these genes was abolished or attenuated under the inactivation of IKK/NF-κB. Among those IKK/NF-κB-dependent genes, 20 genes were NF-κB-inducible genes reported previously, and 59 genes represented putative novel NF-κB target genes. Using transcription factor binding analysis, we found that most of these putative NF-κB target genes contained one or multiple NF-κB-binding sites. Also, some transcription factor-binding motifs were overrepresented in the promoter of both known and putative NF-κB-dependent genes, indicating that these genes may be regulated in a similar fashion. Furthermore, we found that several transcription factors associated with metabolic and inflammatory responses, including nuclear receptors, activator of protein-1, and early growth responses, were induced by P. gingivalis LPS through IKK/NF-κB, indicating that IKK/NF-κB may utilize these transcription factors to mediate secondary responses. Taken together, our results demonstrate that IKK/NF-κB signaling plays a dominant role in P. gingivalis LPS-induced early response gene expression, suggesting that IKK/NF-κB is a therapeutic target for periodontitis. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Biological Chemistry, 2006, v. 281 n. 41, p. 31142-31151 [How to Cite?]
DOI: http://dx.doi.org/10.1074/jbc.M603417200
 
dc.identifier.citeulike11369876
 
dc.identifier.doihttp://dx.doi.org/10.1074/jbc.M603417200
 
dc.identifier.epage31151
 
dc.identifier.hkuros124070
 
dc.identifier.isiWOS:000241075900089
 
dc.identifier.issn0021-9258
2013 Impact Factor: 4.600
 
dc.identifier.issue41
 
dc.identifier.pmid16914552
 
dc.identifier.scopuseid_2-s2.0-33750043186
 
dc.identifier.spage31142
 
dc.identifier.urihttp://hdl.handle.net/10722/154429
 
dc.identifier.volume281
 
dc.languageeng
 
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Biological Chemistry
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshBase Sequence
 
dc.subject.meshGene Expression Regulation
 
dc.subject.meshGenes, Dominant
 
dc.subject.meshHumans
 
dc.subject.meshI-Kappa B Kinase - Metabolism
 
dc.subject.meshLipopolysaccharides - Metabolism
 
dc.subject.meshMice
 
dc.subject.meshMolecular Sequence Data
 
dc.subject.meshMonocytes - Microbiology
 
dc.subject.meshNf-Kappa B - Metabolism
 
dc.subject.meshPorphyromonas Gingivalis - Metabolism
 
dc.subject.meshSignal Transduction
 
dc.titleA dominant function of IKK/NF-κB signaling in global lipopolysaccharide-induced gene expression
 
dc.typeArticle
 
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<contributor.author>States, D</contributor.author>
<contributor.author>Wang, CY</contributor.author>
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<description.abstract>Porphyromonas gingivalis is an etiologic pathogen of periodontitis that is one of the most common inflammatory diseases. Recently, we found that P. gingivalis LPS activated the transcription factor nuclear factor-&#954;B (NF-&#954;B) through the I&#954;B kinase complex (IKK). NF-&#954;B is a transcription factor that controls inflammation and host responses. In this study, we examined the role of IKK/NF-&#954;B in P. gingivalis LPS-induced gene expression on a genome-wide basis using a combination of microarray and biochemical approaches. A total of 88 early response genes were found to be induced by P. gingivalis LPS in a human THP.1 monocytic cell lines. Interestingly, the induction of most of these genes was abolished or attenuated under the inactivation of IKK/NF-&#954;B. Among those IKK/NF-&#954;B-dependent genes, 20 genes were NF-&#954;B-inducible genes reported previously, and 59 genes represented putative novel NF-&#954;B target genes. Using transcription factor binding analysis, we found that most of these putative NF-&#954;B target genes contained one or multiple NF-&#954;B-binding sites. Also, some transcription factor-binding motifs were overrepresented in the promoter of both known and putative NF-&#954;B-dependent genes, indicating that these genes may be regulated in a similar fashion. Furthermore, we found that several transcription factors associated with metabolic and inflammatory responses, including nuclear receptors, activator of protein-1, and early growth responses, were induced by P. gingivalis LPS through IKK/NF-&#954;B, indicating that IKK/NF-&#954;B may utilize these transcription factors to mediate secondary responses. Taken together, our results demonstrate that IKK/NF-&#954;B signaling plays a dominant role in P. gingivalis LPS-induced early response gene expression, suggesting that IKK/NF-&#954;B is a therapeutic target for periodontitis. &#169; 2006 by The American Society for Biochemistry and Molecular Biology, Inc.</description.abstract>
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Author Affiliations
  1. The University of Hong Kong
  2. University Michigan Ann Arbor
  3. University of Michigan School of Dentistry