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Article: Quantitative evaluation of tissue invasion by wild type, hyphal and SAP mutants of Candida albicans, and non-albicans Candida species in reconstituted human oral epithelium

TitleQuantitative evaluation of tissue invasion by wild type, hyphal and SAP mutants of Candida albicans, and non-albicans Candida species in reconstituted human oral epithelium
Authors
Issue Date2006
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOPM
Citation
Journal of Oral Pathology And Medicine, 2006, v. 35 n. 8, p. 484-491 How to Cite?
AbstractBackground: Oral candidiasis is a common problem in compromised patients. Although several non-albicans Candida species have emerged as pathogens the majority of candidal infections are caused by Candida albicans. Morphogenesis from the blastospore to filamentous phase, and production of secretory aspartyl proteinases (SAP) are two major virulence attributes of these opportunistic yeast. Histopathology of oral candidiasis is characterized by fungal invasion of the superficial epithelium although the invasive potentials of different Candida species vary. Computerized image analysis systems (IAS) utilizing immunohistochemistry have been successfully employed for quantification of such histopathological features. The purpose of this study was to evaluate quantitatively the in vitro invasive potential of C. albicans and its hyphal and SAP mutants, and five other non-albicans Candida species using a computerized IAS. Methods: In vitro human oral candidiasis was produced using five wild type and one reference C. albicans isolates, hyphal and SAP mutants of C. albicans SC 5314, and one wild type and one reference isolate each of C. tropicalis, C. dubliniensis, C. glabrata, C. parapsilosis and C. krusei in a reconstituted human oral epithelium (RHOE) model. The infected tissues were examined histologically at 12, 24 and 48 h. Invading fungal elements were visualized by periodic acid-Schiff (PAS) staining and quantitatively evaluated as a percentage of total tissue invasive area, using a computerized IAS. Results: All C. albicans isolates including hyphal mutant cph1/cph1 and SAP mutants; sap 1-3, sap 4-6 produced hyphae and differentially (P < 0.05) invaded the tissue over 48 h. The invasive potential of hyphal mutant cph1/cph1 and SAP mutants (sap 1-3, sap 4-6) were similar to the parent wild-type isolate at 12 h although after 24 h their invasion was dissimilar (P < 0.05). Non-albicans Candida species and hyphal mutants; efg1/efg1, efg1/efg1 cph1/cph1 were all non-invasive. Conclusions: RHOE model in combination with computerized image analysis permits for the first time, the assessment of invasive potential of Candida species in a quantitative manner. The differential tissue invasive patterns of various C. albicans isolates, their mutants and other Candida species are also described. © Blackwell Munksgaard 2006. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/154418
ISSN
2015 Impact Factor: 1.859
2015 SCImago Journal Rankings: 0.731
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJayatilake, JAMSen_US
dc.contributor.authorSamaranayake, YHen_US
dc.contributor.authorCheung, LKen_US
dc.contributor.authorSamaranayake, LPen_US
dc.date.accessioned2012-08-08T08:25:12Z-
dc.date.available2012-08-08T08:25:12Z-
dc.date.issued2006en_US
dc.identifier.citationJournal of Oral Pathology And Medicine, 2006, v. 35 n. 8, p. 484-491en_US
dc.identifier.issn0904-2512en_US
dc.identifier.urihttp://hdl.handle.net/10722/154418-
dc.description.abstractBackground: Oral candidiasis is a common problem in compromised patients. Although several non-albicans Candida species have emerged as pathogens the majority of candidal infections are caused by Candida albicans. Morphogenesis from the blastospore to filamentous phase, and production of secretory aspartyl proteinases (SAP) are two major virulence attributes of these opportunistic yeast. Histopathology of oral candidiasis is characterized by fungal invasion of the superficial epithelium although the invasive potentials of different Candida species vary. Computerized image analysis systems (IAS) utilizing immunohistochemistry have been successfully employed for quantification of such histopathological features. The purpose of this study was to evaluate quantitatively the in vitro invasive potential of C. albicans and its hyphal and SAP mutants, and five other non-albicans Candida species using a computerized IAS. Methods: In vitro human oral candidiasis was produced using five wild type and one reference C. albicans isolates, hyphal and SAP mutants of C. albicans SC 5314, and one wild type and one reference isolate each of C. tropicalis, C. dubliniensis, C. glabrata, C. parapsilosis and C. krusei in a reconstituted human oral epithelium (RHOE) model. The infected tissues were examined histologically at 12, 24 and 48 h. Invading fungal elements were visualized by periodic acid-Schiff (PAS) staining and quantitatively evaluated as a percentage of total tissue invasive area, using a computerized IAS. Results: All C. albicans isolates including hyphal mutant cph1/cph1 and SAP mutants; sap 1-3, sap 4-6 produced hyphae and differentially (P < 0.05) invaded the tissue over 48 h. The invasive potential of hyphal mutant cph1/cph1 and SAP mutants (sap 1-3, sap 4-6) were similar to the parent wild-type isolate at 12 h although after 24 h their invasion was dissimilar (P < 0.05). Non-albicans Candida species and hyphal mutants; efg1/efg1, efg1/efg1 cph1/cph1 were all non-invasive. Conclusions: RHOE model in combination with computerized image analysis permits for the first time, the assessment of invasive potential of Candida species in a quantitative manner. The differential tissue invasive patterns of various C. albicans isolates, their mutants and other Candida species are also described. © Blackwell Munksgaard 2006. All rights reserved.en_US
dc.languageengen_US
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOPMen_US
dc.relation.ispartofJournal of Oral Pathology and Medicineen_US
dc.subject.meshAspartic Acid Endopeptidases - Analysisen_US
dc.subject.meshCandida Albicans - Genetics - Pathogenicityen_US
dc.subject.meshCandidiasis, Oral - Microbiology - Pathologyen_US
dc.subject.meshEpithelium - Microbiology - Pathologyen_US
dc.subject.meshFungal Proteins - Analysisen_US
dc.subject.meshHumansen_US
dc.subject.meshHyphaeen_US
dc.subject.meshStatistics, Nonparametricen_US
dc.titleQuantitative evaluation of tissue invasion by wild type, hyphal and SAP mutants of Candida albicans, and non-albicans Candida species in reconstituted human oral epitheliumen_US
dc.typeArticleen_US
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0904-2512&volume=35&spage=484&epage=91&date=2006&atitle=Quantitative+evaluation+of+tissue+invasion+by+wild+type,+hypal+and+SAP+mutants+of+Candida+albicans,+and+non-albicans+Candida+species+in+reconstituted+human+oral+epithelium-
dc.identifier.emailJayatilake, JAMS: sumedha@hkusua.hku.hken_US
dc.identifier.emailSamaranayake, YH:hema@hkucc.hku.hken_US
dc.identifier.emailCheung, LK:lkcheung@hkucc.hku.hken_US
dc.identifier.emailSamaranayake, LP:lakshman@hku.hk-
dc.identifier.authoritySamaranayake, YH=rp00025en_US
dc.identifier.authorityCheung, LK=rp00013en_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1600-0714.2006.00435.xen_US
dc.identifier.pmid16918600-
dc.identifier.scopuseid_2-s2.0-33747040246en_US
dc.identifier.hkuros116022-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33747040246&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume35en_US
dc.identifier.issue8en_US
dc.identifier.spage484en_US
dc.identifier.epage491en_US
dc.identifier.isiWOS:000239622700005-
dc.publisher.placeDenmarken_US
dc.identifier.scopusauthoridJayatilake, JAMS=8441671500en_US
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_US
dc.identifier.scopusauthoridCheung, LK=7102302747en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US
dc.identifier.citeulike790593-

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