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Article: Effect of a stannous fluoride dentifrice on the sulcular microbiota: a prospective cohort study in subjects with various levels of periodontal inflammation.

TitleEffect of a stannous fluoride dentifrice on the sulcular microbiota: a prospective cohort study in subjects with various levels of periodontal inflammation.
Authors
Issue Date2005
PublisherQuintessence Publishing Co Ltd. The Journal's web site is located at http://www.quintessencepublishing.co.uk/catalog/product_info.php?cPath=3&products_id=239
Citation
Oral Health & Preventive Dentistry., 2005, v. 3 n. 4, p. 263-272 How to Cite?
AbstractOBJECTIVES: To assess the effects of an experimental 0.454% stannous fluoride (SnF2) dentifrice on the oral sulcular microbiota in patients with various stages of oral diseases using checkerboard DNA-DNA hybridization. MATERIAL AND METHODS: In the present one-month, single center, single product, prospective cohort trial, 37 adults (mean age 37.6) were assigned to one of four oral health condition cohorts with seven to 10 subjects each: 1. mild gingivitis, 2. marked generalized gingivitis to moderate periodontitis, 3. caries-prone and 4. treated moderate to advanced chronic periodontitis in supportive periodontal care. All four groups were asked to use the test dentifrice and a power toothbrush twice a day for one minute during a four-week test period. Before and after the trial period, Plaque Indices (PII, Silness and Löe, 1964) and Gingival Indices (GI, Löe and Silness, 1963) were recorded. Subgingival plaque samples were collected from all patients at Baseline, as well as after two and four weeks. These samples were analyzed for content of 40 bacterial species using checkerboard DNA-DNA hybridization. RESULTS: As a result of the only one minute brushing with the stannous fluoride dentifrice, the mean PII at Baseline was significantly lower (p < 0.05) from the mean PII at four weeks. No statistically significant differences were found between premolar and molar mean values. Moreover, no statistically significant differences were found between the mean GI at Baseline and at four weeks. The microbiological analysis showed that at baseline subjects in groups 2 and 4 had significantly higher bacterial loads of bacteria than groups 1, and 3 (i.e. A. actinomyctemcomitans P. gingivalis, T. forsythia, and T. denticola. Over the study period, the total bacterial load did not change in groups 2, 3 and 4. In groups 1 and 3, however, an increase in the loads of Streptococci spp. were noticed (p < 0.05) including S. mitis, S. intermedius, and S. sanguis (p < 0.01) suggesting an increase in the presence of early colonizing and health associated bacteria. CONCLUSION: One minute brushing with a 0.454% stannous fluoride dentifrice did--after four weeks--not affect the subgingival microbial profiles in patients with moderate periodontitis and treated moderate to advanced periodontitis. However, the sulcular microbial profiles of mild gingivitis and caries-prone patients were affected, indicating a shift towards a gingival health associated microbiota in the sulcular region of patients not affected by attachment loss. RUNNING HEAD: Effect of stannous fluoride on sulcular microbiota.
Persistent Identifierhttp://hdl.handle.net/10722/154407
ISSN
2015 Impact Factor: 0.69
2015 SCImago Journal Rankings: 0.325

 

DC FieldValueLanguage
dc.contributor.authorBenjasupattananan, Sen_US
dc.contributor.authorLai, CSen_US
dc.contributor.authorPersson, GRen_US
dc.contributor.authorPjetursson, BEen_US
dc.contributor.authorLang, NPen_US
dc.date.accessioned2012-08-08T08:25:08Z-
dc.date.available2012-08-08T08:25:08Z-
dc.date.issued2005en_US
dc.identifier.citationOral Health & Preventive Dentistry., 2005, v. 3 n. 4, p. 263-272en_US
dc.identifier.issn1602-1622en_US
dc.identifier.urihttp://hdl.handle.net/10722/154407-
dc.description.abstractOBJECTIVES: To assess the effects of an experimental 0.454% stannous fluoride (SnF2) dentifrice on the oral sulcular microbiota in patients with various stages of oral diseases using checkerboard DNA-DNA hybridization. MATERIAL AND METHODS: In the present one-month, single center, single product, prospective cohort trial, 37 adults (mean age 37.6) were assigned to one of four oral health condition cohorts with seven to 10 subjects each: 1. mild gingivitis, 2. marked generalized gingivitis to moderate periodontitis, 3. caries-prone and 4. treated moderate to advanced chronic periodontitis in supportive periodontal care. All four groups were asked to use the test dentifrice and a power toothbrush twice a day for one minute during a four-week test period. Before and after the trial period, Plaque Indices (PII, Silness and Löe, 1964) and Gingival Indices (GI, Löe and Silness, 1963) were recorded. Subgingival plaque samples were collected from all patients at Baseline, as well as after two and four weeks. These samples were analyzed for content of 40 bacterial species using checkerboard DNA-DNA hybridization. RESULTS: As a result of the only one minute brushing with the stannous fluoride dentifrice, the mean PII at Baseline was significantly lower (p < 0.05) from the mean PII at four weeks. No statistically significant differences were found between premolar and molar mean values. Moreover, no statistically significant differences were found between the mean GI at Baseline and at four weeks. The microbiological analysis showed that at baseline subjects in groups 2 and 4 had significantly higher bacterial loads of bacteria than groups 1, and 3 (i.e. A. actinomyctemcomitans P. gingivalis, T. forsythia, and T. denticola. Over the study period, the total bacterial load did not change in groups 2, 3 and 4. In groups 1 and 3, however, an increase in the loads of Streptococci spp. were noticed (p < 0.05) including S. mitis, S. intermedius, and S. sanguis (p < 0.01) suggesting an increase in the presence of early colonizing and health associated bacteria. CONCLUSION: One minute brushing with a 0.454% stannous fluoride dentifrice did--after four weeks--not affect the subgingival microbial profiles in patients with moderate periodontitis and treated moderate to advanced periodontitis. However, the sulcular microbial profiles of mild gingivitis and caries-prone patients were affected, indicating a shift towards a gingival health associated microbiota in the sulcular region of patients not affected by attachment loss. RUNNING HEAD: Effect of stannous fluoride on sulcular microbiota.en_US
dc.languageengen_US
dc.publisherQuintessence Publishing Co Ltd. The Journal's web site is located at http://www.quintessencepublishing.co.uk/catalog/product_info.php?cPath=3&products_id=239en_US
dc.relation.ispartofOral health & preventive dentistry.en_US
dc.subject.meshActinobacillus Actinomycetemcomitans - Drug Effectsen_US
dc.subject.meshAdulten_US
dc.subject.meshBacteria - Classification - Drug Effectsen_US
dc.subject.meshBacteroides - Drug Effectsen_US
dc.subject.meshCariostatic Agents - Administration & Dosage - Therapeutic Useen_US
dc.subject.meshCohort Studiesen_US
dc.subject.meshDental Caries - Microbiology - Physiopathologyen_US
dc.subject.meshDental Plaque - Microbiologyen_US
dc.subject.meshDentifrices - Therapeutic Useen_US
dc.subject.meshFollow-Up Studiesen_US
dc.subject.meshGingiva - Microbiologyen_US
dc.subject.meshGingivitis - Microbiology - Physiopathologyen_US
dc.subject.meshHumansen_US
dc.subject.meshPeriodontitis - Classification - Microbiologyen_US
dc.subject.meshPorphyromonas Gingivalis - Drug Effectsen_US
dc.subject.meshProspective Studiesen_US
dc.subject.meshStreptococcus Intermedius - Drug Effectsen_US
dc.subject.meshStreptococcus Mitis - Drug Effectsen_US
dc.subject.meshStreptococcus Sanguis - Drug Effectsen_US
dc.subject.meshTin Fluorides - Administration & Dosage - Therapeutic Useen_US
dc.subject.meshToothbrushing - Instrumentationen_US
dc.subject.meshTreponema Denticola - Drug Effectsen_US
dc.titleEffect of a stannous fluoride dentifrice on the sulcular microbiota: a prospective cohort study in subjects with various levels of periodontal inflammation.en_US
dc.typeArticleen_US
dc.identifier.emailLang, NP:nplang@hkucc.hku.hken_US
dc.identifier.authorityLang, NP=rp00031en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid16475455-
dc.identifier.scopuseid_2-s2.0-33646711686en_US
dc.identifier.volume3en_US
dc.identifier.issue4en_US
dc.identifier.spage263en_US
dc.identifier.epage272en_US
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridBenjasupattananan, S=13604768600en_US
dc.identifier.scopusauthoridLai, CS=13605682900en_US
dc.identifier.scopusauthoridPersson, GR=7101853867en_US
dc.identifier.scopusauthoridPjetursson, BE=6506841442en_US
dc.identifier.scopusauthoridLang, NP=7201577367en_US

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