File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Expression of human β-defensins-1 and -2 peptides in unresolved chronic periodontitis

TitleExpression of human β-defensins-1 and -2 peptides in unresolved chronic periodontitis
Authors
Issue Date2004
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
Citation
Journal Of Periodontal Research, 2004, v. 39 n. 4, p. 221-227 How to Cite?
AbstractBackground: Human β-defensins (hBDs) are antimicrobial peptides which contribute to host innate immunity by disrupting the membrane integrity of a broad spectrum of microorganisms. Objectives: This study aimed to determine the expression profiles of hBD-1 and -2 peptides in gingiva and to assess the possible relations of these antimicrobial peptides with periodontal health and disease. Methods: Seven periodontally healthy subjects and 22 patients with unresolved chronic periodontitis were recruited and the gingival biopsies collected consisted of healthy tissues from the healthy subjects (HT-C); periodontal pocket tissues (PoT) and inflamed connective tissues (ICT) from the base of pocket, i.e. granulation tissues, as well as clinically healthy tissues (HT-P) from the adjacent clinically healthy sites from the patients. The expression of hBD-1 and -2 peptides was detected by immunohistochemistry and quantitatively analyzed with a computerized image processing system. Results: Both hBD-1 and -2 peptides were detected in all periodontally healthy subjects, while hBD-1 was detected in all patients and hBD-2 was found in most of the patients. Their expression was mainly confined to the granular and spinous layers of gingival epithelium, in which hBD-1 was detected in both intercellular spaces and cytoplasm, whereas hBD-2 was mainly observed in the cytoplasm. HT-C expressed significantly higher levels of hBD-2 than HT-P (p < 0.05). Within the patients, both defensins were up-regulated significantly in PoT as compared with the adjacent HT-P (p < 0.05). Conclusions: The present study showed that hBD-1 and -2 were frequently expressed in the granular and spinous layers of gingival epithelia and their expression may be associated with periodontal health and disease. © Blackwell Munksgaard, 2004.
Persistent Identifierhttp://hdl.handle.net/10722/154382
ISSN
2015 Impact Factor: 2.474
2015 SCImago Journal Rankings: 0.932
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLu, Qen_US
dc.contributor.authorJin, Len_US
dc.contributor.authorDarveau, RPen_US
dc.contributor.authorSamaranayake, LPen_US
dc.date.accessioned2012-08-08T08:25:00Z-
dc.date.available2012-08-08T08:25:00Z-
dc.date.issued2004en_US
dc.identifier.citationJournal Of Periodontal Research, 2004, v. 39 n. 4, p. 221-227en_US
dc.identifier.issn0022-3484en_US
dc.identifier.urihttp://hdl.handle.net/10722/154382-
dc.description.abstractBackground: Human β-defensins (hBDs) are antimicrobial peptides which contribute to host innate immunity by disrupting the membrane integrity of a broad spectrum of microorganisms. Objectives: This study aimed to determine the expression profiles of hBD-1 and -2 peptides in gingiva and to assess the possible relations of these antimicrobial peptides with periodontal health and disease. Methods: Seven periodontally healthy subjects and 22 patients with unresolved chronic periodontitis were recruited and the gingival biopsies collected consisted of healthy tissues from the healthy subjects (HT-C); periodontal pocket tissues (PoT) and inflamed connective tissues (ICT) from the base of pocket, i.e. granulation tissues, as well as clinically healthy tissues (HT-P) from the adjacent clinically healthy sites from the patients. The expression of hBD-1 and -2 peptides was detected by immunohistochemistry and quantitatively analyzed with a computerized image processing system. Results: Both hBD-1 and -2 peptides were detected in all periodontally healthy subjects, while hBD-1 was detected in all patients and hBD-2 was found in most of the patients. Their expression was mainly confined to the granular and spinous layers of gingival epithelium, in which hBD-1 was detected in both intercellular spaces and cytoplasm, whereas hBD-2 was mainly observed in the cytoplasm. HT-C expressed significantly higher levels of hBD-2 than HT-P (p < 0.05). Within the patients, both defensins were up-regulated significantly in PoT as compared with the adjacent HT-P (p < 0.05). Conclusions: The present study showed that hBD-1 and -2 were frequently expressed in the granular and spinous layers of gingival epithelia and their expression may be associated with periodontal health and disease. © Blackwell Munksgaard, 2004.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1en_US
dc.relation.ispartofJournal of Periodontal Researchen_US
dc.subject.meshAnti-Infective Agents - Analysisen_US
dc.subject.meshChronic Diseaseen_US
dc.subject.meshCytoplasm - Ultrastructureen_US
dc.subject.meshEpithelium - Pathologyen_US
dc.subject.meshExtracellular Spaceen_US
dc.subject.meshGingiva - Pathologyen_US
dc.subject.meshGranulation Tissue - Pathologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImage Processing, Computer-Assisteden_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshPeriodontal Pocket - Pathologyen_US
dc.subject.meshPeriodontitis - Pathologyen_US
dc.subject.meshPeriodontium - Pathologyen_US
dc.subject.meshUp-Regulationen_US
dc.subject.meshBeta-Defensins - Analysisen_US
dc.titleExpression of human β-defensins-1 and -2 peptides in unresolved chronic periodontitisen_US
dc.typeArticleen_US
dc.identifier.emailJin, L:ljjin@hkucc.hku.hken_US
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_US
dc.identifier.authorityJin, L=rp00028en_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1600-0765.2004.00727.xen_US
dc.identifier.pmid15206914-
dc.identifier.scopuseid_2-s2.0-3242751259en_US
dc.identifier.hkuros88957-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-3242751259&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume39en_US
dc.identifier.issue4en_US
dc.identifier.spage221en_US
dc.identifier.epage227en_US
dc.identifier.isiWOS:000222175200003-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLu, Q=36128876000en_US
dc.identifier.scopusauthoridJin, L=7403328850en_US
dc.identifier.scopusauthoridDarveau, RP=7006419856en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats