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Article: The expression profile of lipopolysaccharide-binding protein, membrane-bound CD14, and toll-like receptors 2 and 4 in chronic periodontitis

TitleThe expression profile of lipopolysaccharide-binding protein, membrane-bound CD14, and toll-like receptors 2 and 4 in chronic periodontitis
Authors
Issue Date2005
PublisherAmerican Academy of Periodontology. The Journal's web site is located at http://www.perio.org
Citation
Journal of Periodontology, 2005, v. 76 n. 11, p. 1950-1959 How to Cite?
AbstractBackground: This study aimed to investigate the interrelationship of in vivo expression of lipopolysaccharide-binding protein (LBP) and membrane-bound CD14 (mCD14) in human gingival tissues as well as the coexpression of Toll-like receptors (TLR) 2 and 4 in association with periodontal conditions. Methods: Gingival biopsies were collected from 43 subjects with chronic periodontitis, including periodontal pocket tissues (PoTs) and clinically healthy gingival tissues (HT-Ps), and from 15 periodontally healthy subjects as controls (HT-Cs). The expression of LBP, CD14, TLR 2, and TLR 4 was detected by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Results: LBP and mCD14 peptides were simultaneously detected in 91% of PoTs, 85% of HT-Ps, and 100% of HT-Cs. LBP and mCD14 mRNAs were simultaneously detected in 55% of PoTs, 55% of HT-Ps, and 75% of HT-Cs. The expression of LBP was confined to the gingival epithelium, whereas mCD14 was observed around the epithelium-connective tissue interface. A positive correlation existed between LBP and mCD14 peptides in both detection expression (rs = 0.608; P <0.001) and expression levels (r = 0.304; P <0.05) of these two molecules. In PoTs, TLR 2 was detected in both pocket epithelia and macrophage-like cells in connective tissues, whereas TLR 4 was predominantly detected in connective tissues. In HT-Ps and HT-Cs, a weak expression of TLR 2 was found in gingival epithelia, and no TLR 4 expression was detected. In PoTs, mCD14 was codetected on CD68-labeled macrophages in the underlying connective tissues of pocket epithelium as well as on CD1a-labeled dendritic cells in the pocket epithelium and connective tissues interface. No similar expression profile was detected in HT-Ps and HT-Cs. Conclusions: This study suggests that the in vivo expression of LBP and mCD14 may be interrelated. Altered cellular expression profiles of mCD14 and TLR 2 and 4 in periodontal pocket tissues imply that these pattern recognition receptors may play a role in periodontal pathogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/154373
ISSN
2015 Impact Factor: 2.844
2015 SCImago Journal Rankings: 1.070
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorRen, Len_US
dc.contributor.authorLeung, WKen_US
dc.contributor.authorDarveau, RPen_US
dc.contributor.authorJin, Len_US
dc.date.accessioned2012-08-08T08:24:57Z-
dc.date.available2012-08-08T08:24:57Z-
dc.date.issued2005en_US
dc.identifier.citationJournal of Periodontology, 2005, v. 76 n. 11, p. 1950-1959en_US
dc.identifier.issn0022-3492en_US
dc.identifier.urihttp://hdl.handle.net/10722/154373-
dc.description.abstractBackground: This study aimed to investigate the interrelationship of in vivo expression of lipopolysaccharide-binding protein (LBP) and membrane-bound CD14 (mCD14) in human gingival tissues as well as the coexpression of Toll-like receptors (TLR) 2 and 4 in association with periodontal conditions. Methods: Gingival biopsies were collected from 43 subjects with chronic periodontitis, including periodontal pocket tissues (PoTs) and clinically healthy gingival tissues (HT-Ps), and from 15 periodontally healthy subjects as controls (HT-Cs). The expression of LBP, CD14, TLR 2, and TLR 4 was detected by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Results: LBP and mCD14 peptides were simultaneously detected in 91% of PoTs, 85% of HT-Ps, and 100% of HT-Cs. LBP and mCD14 mRNAs were simultaneously detected in 55% of PoTs, 55% of HT-Ps, and 75% of HT-Cs. The expression of LBP was confined to the gingival epithelium, whereas mCD14 was observed around the epithelium-connective tissue interface. A positive correlation existed between LBP and mCD14 peptides in both detection expression (rs = 0.608; P <0.001) and expression levels (r = 0.304; P <0.05) of these two molecules. In PoTs, TLR 2 was detected in both pocket epithelia and macrophage-like cells in connective tissues, whereas TLR 4 was predominantly detected in connective tissues. In HT-Ps and HT-Cs, a weak expression of TLR 2 was found in gingival epithelia, and no TLR 4 expression was detected. In PoTs, mCD14 was codetected on CD68-labeled macrophages in the underlying connective tissues of pocket epithelium as well as on CD1a-labeled dendritic cells in the pocket epithelium and connective tissues interface. No similar expression profile was detected in HT-Ps and HT-Cs. Conclusions: This study suggests that the in vivo expression of LBP and mCD14 may be interrelated. Altered cellular expression profiles of mCD14 and TLR 2 and 4 in periodontal pocket tissues imply that these pattern recognition receptors may play a role in periodontal pathogenesis.en_US
dc.languageengen_US
dc.publisherAmerican Academy of Periodontology. The Journal's web site is located at http://www.perio.orgen_US
dc.relation.ispartofJournal of Periodontologyen_US
dc.subject.meshAcute-Phase Proteins - Analysisen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAlveolar Bone Loss - Pathologyen_US
dc.subject.meshAntigens, Cd14 - Analysisen_US
dc.subject.meshBiopsyen_US
dc.subject.meshCarrier Proteins - Analysisen_US
dc.subject.meshChronic Diseaseen_US
dc.subject.meshConnective Tissue - Pathologyen_US
dc.subject.meshDendritic Cells - Pathologyen_US
dc.subject.meshEpithelium - Pathologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGingiva - Pathologyen_US
dc.subject.meshHumansen_US
dc.subject.meshLipopolysaccharides - Analysisen_US
dc.subject.meshMacrophages - Pathologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Glycoproteins - Analysisen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPeriodontal Attachment Loss - Pathologyen_US
dc.subject.meshPeriodontal Pocket - Pathologyen_US
dc.subject.meshPeriodontitis - Pathologyen_US
dc.subject.meshToll-Like Receptor 2 - Analysisen_US
dc.subject.meshToll-Like Receptor 4 - Analysisen_US
dc.titleThe expression profile of lipopolysaccharide-binding protein, membrane-bound CD14, and toll-like receptors 2 and 4 in chronic periodontitisen_US
dc.typeArticleen_US
dc.identifier.emailRen, L: h0196804@hkusua.hku.hken_US
dc.identifier.emailLeung, WK: ewkleung@hkucc.hku.hken_US
dc.identifier.emailJin, L: ljjin@hkucc.hku.hk-
dc.identifier.authorityLeung, WK=rp00019en_US
dc.identifier.authorityJin, L=rp00028en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1902/jop.2005.76.11.1950en_US
dc.identifier.pmid16274315-
dc.identifier.scopuseid_2-s2.0-28644452355en_US
dc.identifier.hkuros112530-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-28644452355&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume76en_US
dc.identifier.issue11en_US
dc.identifier.spage1950en_US
dc.identifier.epage1959en_US
dc.identifier.isiWOS:000233396300018-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridRen, L=35216223200en_US
dc.identifier.scopusauthoridLeung, WK=25224691800en_US
dc.identifier.scopusauthoridDarveau, RP=7006419856en_US
dc.identifier.scopusauthoridJin, L=7403328850en_US

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