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Article: Sequence of protein expression of bone sialoprotein and osteopontin at the developing interface between repair cementum and dentin in human deciduous teeth

TitleSequence of protein expression of bone sialoprotein and osteopontin at the developing interface between repair cementum and dentin in human deciduous teeth
Authors
Issue Date2005
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm
Citation
Cell And Tissue Research, 2005, v. 320 n. 3, p. 399-407 How to Cite?
AbstractExperimental periodontal regeneration studies have revealed the weak binding of repair cementum to the root surface, whereas attachment of cementum to dentin preconditioned by odontoclasts appears to be superior. The aim of this study has been, therefore, to analyze the structural and partial biochemical nature of the interface that develops between resorbed dentin and repair cementum by using human deciduous teeth as a model. Aldehyde-fixed and decalcified tooth samples were embedded in acrylic or epoxy resins and sectioned for light and transmission electron microscopy. Antibodies against bone sialoprotein (BSP) and osteopontin (OPN), two noncollagenous proteins accumulating at hard tissue interfaces in bone and teeth, were used for protein A-gold immunocytochemistry. Light microscopy revealed a gradually increasing staining intensity of the external dentin matrix starting after the withdrawal of the odontoclast. Labeling for both BSP and OPN was first detected among the exposed collagen fibrils and in the intratubular dentin matrix when odontoclasts had withdrawn but mesenchymal cells were present. Subsequently, collagen fibrils of the repair cementum were deposited concomitantly with the appearance of labeling for BSP and OPN over the intratubular, intertubular, and peritubular dentin matrix. Labeled mineralization foci indicated the advancing mineralization front, and the collagenous repair matrix became integrated in an electron-dense organic material that showed labeling for BSP and OPN. Thus, no distinct planar interfacial matrix layer lies between the resorbed dentin and the repair cementum. The results suggest that odontoclasts precondition the dentin matrix such that the repair cementum becomes firmly attached. © Springer-Verlag 2005.
Persistent Identifierhttp://hdl.handle.net/10722/154334
ISSN
2015 Impact Factor: 2.948
2015 SCImago Journal Rankings: 1.539
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorBosshardt, DDen_US
dc.contributor.authorDegen, Ten_US
dc.contributor.authorLang, NPen_US
dc.date.accessioned2012-08-08T08:24:40Z-
dc.date.available2012-08-08T08:24:40Z-
dc.date.issued2005en_US
dc.identifier.citationCell And Tissue Research, 2005, v. 320 n. 3, p. 399-407en_US
dc.identifier.issn0302-766Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/154334-
dc.description.abstractExperimental periodontal regeneration studies have revealed the weak binding of repair cementum to the root surface, whereas attachment of cementum to dentin preconditioned by odontoclasts appears to be superior. The aim of this study has been, therefore, to analyze the structural and partial biochemical nature of the interface that develops between resorbed dentin and repair cementum by using human deciduous teeth as a model. Aldehyde-fixed and decalcified tooth samples were embedded in acrylic or epoxy resins and sectioned for light and transmission electron microscopy. Antibodies against bone sialoprotein (BSP) and osteopontin (OPN), two noncollagenous proteins accumulating at hard tissue interfaces in bone and teeth, were used for protein A-gold immunocytochemistry. Light microscopy revealed a gradually increasing staining intensity of the external dentin matrix starting after the withdrawal of the odontoclast. Labeling for both BSP and OPN was first detected among the exposed collagen fibrils and in the intratubular dentin matrix when odontoclasts had withdrawn but mesenchymal cells were present. Subsequently, collagen fibrils of the repair cementum were deposited concomitantly with the appearance of labeling for BSP and OPN over the intratubular, intertubular, and peritubular dentin matrix. Labeled mineralization foci indicated the advancing mineralization front, and the collagenous repair matrix became integrated in an electron-dense organic material that showed labeling for BSP and OPN. Thus, no distinct planar interfacial matrix layer lies between the resorbed dentin and the repair cementum. The results suggest that odontoclasts precondition the dentin matrix such that the repair cementum becomes firmly attached. © Springer-Verlag 2005.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htmen_US
dc.relation.ispartofCell and Tissue Researchen_US
dc.subject.meshAdolescenten_US
dc.subject.meshCementogenesisen_US
dc.subject.meshChilden_US
dc.subject.meshDental Cementum - Metabolism - Pathologyen_US
dc.subject.meshDentin - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshIntegrin-Binding Sialoproteinen_US
dc.subject.meshMaleen_US
dc.subject.meshMicroscopy, Immunoelectronen_US
dc.subject.meshOsteopontinen_US
dc.subject.meshRoot Resorption - Metabolism - Pathologyen_US
dc.subject.meshSialoglycoproteins - Biosynthesisen_US
dc.subject.meshTooth, Deciduous - Metabolism - Pathologyen_US
dc.titleSequence of protein expression of bone sialoprotein and osteopontin at the developing interface between repair cementum and dentin in human deciduous teethen_US
dc.typeArticleen_US
dc.identifier.emailLang, NP:nplang@hkucc.hku.hken_US
dc.identifier.authorityLang, NP=rp00031en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00441-005-1106-8en_US
dc.identifier.pmid15846504-
dc.identifier.scopuseid_2-s2.0-21244476361en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-21244476361&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume320en_US
dc.identifier.issue3en_US
dc.identifier.spage399en_US
dc.identifier.epage407en_US
dc.identifier.isiWOS:000229511300004-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridBosshardt, DD=6603806230en_US
dc.identifier.scopusauthoridDegen, T=36840643900en_US
dc.identifier.scopusauthoridLang, NP=7201577367en_US

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