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Article: Effects of enamel matrix proteins on tissue formation along the roots of human teeth
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TitleEffects of enamel matrix proteins on tissue formation along the roots of human teeth
 
AuthorsBosshardt, DD2
Sculean, A1
Windisch, P3
Pjetursson, BE
Lang, NP
 
Issue Date2005
 
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
 
CitationJournal Of Periodontal Research, 2005, v. 40 n. 2, p. 158-167 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-0765.2005.00785.x
 
AbstractObjective: Enamel matrix-derived proteins (EMD) are thought to trigger the formation of acellular extrinsic fibre cementum (AEFC), while other reports indicate that EMD may have osteogenic potential. The aim of the present study was to characterize the tissues developing on the root surface following application of EMD. Methods: Twelve human periodontitis-affected teeth, scheduled for extraction, were treated with EMD. Two to 6 weeks later, the teeth were extracted, demineralized and processed for embedding in acrylic and epoxy resins. New tissue formation was analysed by light and transmission electron microscopy. Results: New tissue formation on the root was observed in the notch and on both scaled and unscaled root surfaces distant of the notch area in six defects. The newly formed tissues on the root were thick, collagenous, devoid of extrinsic fibres, and had an irregular surface contour. The presence of electron-dense, organic material in the collagenous matrix indicated at least partial mineralization. Embedded cells were numerous and the cells on the matrix surface were very large in size. Abundant rough endoplasmic reticulum and a prominent Golgi complex were evident. The presence of a split between the treated root surfaces and the newly formed tissue was a common observation, as was the presence of bacteria and host cells in the interfacial gap. Conclusion: Following treatment with EMD, a bone-like tissue resembling cellular intrinsic fibre cementum may develop on the root surfaces, instead of AEFC. Furthermore, EMD may both induce de novo formation of a mineralized connective tissue on scaled root surfaces and stimulate matrix deposition on old native cementum. Interfacial bonding appeared to be weak after 6 weeks of healing. Copyright © Blackwell Munksgaard Ltd.
 
ISSN0022-3484
2013 Impact Factor: 2.215
 
DOIhttp://dx.doi.org/10.1111/j.1600-0765.2005.00785.x
 
ISI Accession Number IDWOS:000227878600009
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorBosshardt, DD
 
dc.contributor.authorSculean, A
 
dc.contributor.authorWindisch, P
 
dc.contributor.authorPjetursson, BE
 
dc.contributor.authorLang, NP
 
dc.date.accessioned2012-08-08T08:24:30Z
 
dc.date.available2012-08-08T08:24:30Z
 
dc.date.issued2005
 
dc.description.abstractObjective: Enamel matrix-derived proteins (EMD) are thought to trigger the formation of acellular extrinsic fibre cementum (AEFC), while other reports indicate that EMD may have osteogenic potential. The aim of the present study was to characterize the tissues developing on the root surface following application of EMD. Methods: Twelve human periodontitis-affected teeth, scheduled for extraction, were treated with EMD. Two to 6 weeks later, the teeth were extracted, demineralized and processed for embedding in acrylic and epoxy resins. New tissue formation was analysed by light and transmission electron microscopy. Results: New tissue formation on the root was observed in the notch and on both scaled and unscaled root surfaces distant of the notch area in six defects. The newly formed tissues on the root were thick, collagenous, devoid of extrinsic fibres, and had an irregular surface contour. The presence of electron-dense, organic material in the collagenous matrix indicated at least partial mineralization. Embedded cells were numerous and the cells on the matrix surface were very large in size. Abundant rough endoplasmic reticulum and a prominent Golgi complex were evident. The presence of a split between the treated root surfaces and the newly formed tissue was a common observation, as was the presence of bacteria and host cells in the interfacial gap. Conclusion: Following treatment with EMD, a bone-like tissue resembling cellular intrinsic fibre cementum may develop on the root surfaces, instead of AEFC. Furthermore, EMD may both induce de novo formation of a mineralized connective tissue on scaled root surfaces and stimulate matrix deposition on old native cementum. Interfacial bonding appeared to be weak after 6 weeks of healing. Copyright © Blackwell Munksgaard Ltd.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Periodontal Research, 2005, v. 40 n. 2, p. 158-167 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-0765.2005.00785.x
 
dc.identifier.doihttp://dx.doi.org/10.1111/j.1600-0765.2005.00785.x
 
dc.identifier.epage167
 
dc.identifier.isiWOS:000227878600009
 
dc.identifier.issn0022-3484
2013 Impact Factor: 2.215
 
dc.identifier.issue2
 
dc.identifier.pmid15733151
 
dc.identifier.scopuseid_2-s2.0-15744391739
 
dc.identifier.spage158
 
dc.identifier.urihttp://hdl.handle.net/10722/154302
 
dc.identifier.volume40
 
dc.languageeng
 
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Periodontal Research
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshCementogenesis - Physiology
 
dc.subject.meshDental Cementum - Drug Effects
 
dc.subject.meshDental Enamel Proteins - Therapeutic Use
 
dc.subject.meshFemale
 
dc.subject.meshGuided Tissue Regeneration - Methods
 
dc.subject.meshHumans
 
dc.subject.meshMale
 
dc.subject.meshMicroscopy, Electron
 
dc.subject.meshMiddle Aged
 
dc.subject.meshPeriodontitis - Therapy
 
dc.subject.meshTooth Root - Drug Effects - Physiology - Ultrastructure
 
dc.titleEffects of enamel matrix proteins on tissue formation along the roots of human teeth
 
dc.typeArticle
 
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<contributor.author>Pjetursson, BE</contributor.author>
<contributor.author>Lang, NP</contributor.author>
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<description.abstract>Objective: Enamel matrix-derived proteins (EMD) are thought to trigger the formation of acellular extrinsic fibre cementum (AEFC), while other reports indicate that EMD may have osteogenic potential. The aim of the present study was to characterize the tissues developing on the root surface following application of EMD. Methods: Twelve human periodontitis-affected teeth, scheduled for extraction, were treated with EMD. Two to 6 weeks later, the teeth were extracted, demineralized and processed for embedding in acrylic and epoxy resins. New tissue formation was analysed by light and transmission electron microscopy. Results: New tissue formation on the root was observed in the notch and on both scaled and unscaled root surfaces distant of the notch area in six defects. The newly formed tissues on the root were thick, collagenous, devoid of extrinsic fibres, and had an irregular surface contour. The presence of electron-dense, organic material in the collagenous matrix indicated at least partial mineralization. Embedded cells were numerous and the cells on the matrix surface were very large in size. Abundant rough endoplasmic reticulum and a prominent Golgi complex were evident. The presence of a split between the treated root surfaces and the newly formed tissue was a common observation, as was the presence of bacteria and host cells in the interfacial gap. Conclusion: Following treatment with EMD, a bone-like tissue resembling cellular intrinsic fibre cementum may develop on the root surfaces, instead of AEFC. Furthermore, EMD may both induce de novo formation of a mineralized connective tissue on scaled root surfaces and stimulate matrix deposition on old native cementum. Interfacial bonding appeared to be weak after 6 weeks of healing. Copyright &#169; Blackwell Munksgaard Ltd.</description.abstract>
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Author Affiliations
  1. Radboud University Nijmegen Medical Centre
  2. Universität Bern
  3. Semmelweis Egyetem