Article: Effects of enamel matrix proteins on tissue formation along the roots of human teeth

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TitleEffects of enamel matrix proteins on tissue formation along the roots of human teeth
AuthorsBosshardt, DD2
Sculean, A1
Windisch, P3
Pjetursson, BE
Lang, NP
Issue Date2005
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
CitationJournal Of Periodontal Research, 2005, v. 40 n. 2, p. 158-167 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-0765.2005.00785.x
AbstractObjective: Enamel matrix-derived proteins (EMD) are thought to trigger the formation of acellular extrinsic fibre cementum (AEFC), while other reports indicate that EMD may have osteogenic potential. The aim of the present study was to characterize the tissues developing on the root surface following application of EMD. Methods: Twelve human periodontitis-affected teeth, scheduled for extraction, were treated with EMD. Two to 6 weeks later, the teeth were extracted, demineralized and processed for embedding in acrylic and epoxy resins. New tissue formation was analysed by light and transmission electron microscopy. Results: New tissue formation on the root was observed in the notch and on both scaled and unscaled root surfaces distant of the notch area in six defects. The newly formed tissues on the root were thick, collagenous, devoid of extrinsic fibres, and had an irregular surface contour. The presence of electron-dense, organic material in the collagenous matrix indicated at least partial mineralization. Embedded cells were numerous and the cells on the matrix surface were very large in size. Abundant rough endoplasmic reticulum and a prominent Golgi complex were evident. The presence of a split between the treated root surfaces and the newly formed tissue was a common observation, as was the presence of bacteria and host cells in the interfacial gap. Conclusion: Following treatment with EMD, a bone-like tissue resembling cellular intrinsic fibre cementum may develop on the root surfaces, instead of AEFC. Furthermore, EMD may both induce de novo formation of a mineralized connective tissue on scaled root surfaces and stimulate matrix deposition on old native cementum. Interfacial bonding appeared to be weak after 6 weeks of healing. Copyright © Blackwell Munksgaard Ltd.
ISSN0022-3484
2011 Impact Factor: 1.686
2011 SCImago Journal Rankings: 0.120
DOIhttp://dx.doi.org/10.1111/j.1600-0765.2005.00785.x
ISI Accession Number IDWOS:000227878600009
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorBosshardt, DD
dc.contributor.authorSculean, A
dc.contributor.authorWindisch, P
dc.contributor.authorPjetursson, BE
dc.contributor.authorLang, NP
dc.date.accessioned2012-08-08T08:24:30Z
dc.date.available2012-08-08T08:24:30Z
dc.date.issued2005
dc.description.abstractObjective: Enamel matrix-derived proteins (EMD) are thought to trigger the formation of acellular extrinsic fibre cementum (AEFC), while other reports indicate that EMD may have osteogenic potential. The aim of the present study was to characterize the tissues developing on the root surface following application of EMD. Methods: Twelve human periodontitis-affected teeth, scheduled for extraction, were treated with EMD. Two to 6 weeks later, the teeth were extracted, demineralized and processed for embedding in acrylic and epoxy resins. New tissue formation was analysed by light and transmission electron microscopy. Results: New tissue formation on the root was observed in the notch and on both scaled and unscaled root surfaces distant of the notch area in six defects. The newly formed tissues on the root were thick, collagenous, devoid of extrinsic fibres, and had an irregular surface contour. The presence of electron-dense, organic material in the collagenous matrix indicated at least partial mineralization. Embedded cells were numerous and the cells on the matrix surface were very large in size. Abundant rough endoplasmic reticulum and a prominent Golgi complex were evident. The presence of a split between the treated root surfaces and the newly formed tissue was a common observation, as was the presence of bacteria and host cells in the interfacial gap. Conclusion: Following treatment with EMD, a bone-like tissue resembling cellular intrinsic fibre cementum may develop on the root surfaces, instead of AEFC. Furthermore, EMD may both induce de novo formation of a mineralized connective tissue on scaled root surfaces and stimulate matrix deposition on old native cementum. Interfacial bonding appeared to be weak after 6 weeks of healing. Copyright © Blackwell Munksgaard Ltd.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Periodontal Research, 2005, v. 40 n. 2, p. 158-167 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-0765.2005.00785.x
dc.identifier.doihttp://dx.doi.org/10.1111/j.1600-0765.2005.00785.x
dc.identifier.epage167
dc.identifier.isiWOS:000227878600009
dc.identifier.issn0022-3484
2011 Impact Factor: 1.686
2011 SCImago Journal Rankings: 0.120
dc.identifier.issue2
dc.identifier.pmid15733151
dc.identifier.scopuseid_2-s2.0-15744391739
dc.identifier.spage158
dc.identifier.urihttp://hdl.handle.net/10722/154302
dc.identifier.volume40
dc.languageeng
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
dc.publisher.placeUnited States
dc.relation.ispartofJournal of Periodontal Research
dc.relation.referencesReferences in Scopus
dc.subject.meshCementogenesis - Physiology
dc.subject.meshDental Cementum - Drug Effects
dc.subject.meshDental Enamel Proteins - Therapeutic Use
dc.subject.meshFemale
dc.subject.meshGuided Tissue Regeneration - Methods
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMicroscopy, Electron
dc.subject.meshMiddle Aged
dc.subject.meshPeriodontitis - Therapy
dc.subject.meshTooth Root - Drug Effects - Physiology - Ultrastructure
dc.titleEffects of enamel matrix proteins on tissue formation along the roots of human teeth
dc.typeArticle
Author Affiliations
  1. Radboud University Nijmegen Medical Centre
  2. Universität Bern
  3. Semmelweis Egyetem