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Article: Effects of enamel matrix proteins on tissue formation along the roots of human teeth

TitleEffects of enamel matrix proteins on tissue formation along the roots of human teeth
Authors
Issue Date2005
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
Citation
Journal Of Periodontal Research, 2005, v. 40 n. 2, p. 158-167 How to Cite?
Abstract
Objective: Enamel matrix-derived proteins (EMD) are thought to trigger the formation of acellular extrinsic fibre cementum (AEFC), while other reports indicate that EMD may have osteogenic potential. The aim of the present study was to characterize the tissues developing on the root surface following application of EMD. Methods: Twelve human periodontitis-affected teeth, scheduled for extraction, were treated with EMD. Two to 6 weeks later, the teeth were extracted, demineralized and processed for embedding in acrylic and epoxy resins. New tissue formation was analysed by light and transmission electron microscopy. Results: New tissue formation on the root was observed in the notch and on both scaled and unscaled root surfaces distant of the notch area in six defects. The newly formed tissues on the root were thick, collagenous, devoid of extrinsic fibres, and had an irregular surface contour. The presence of electron-dense, organic material in the collagenous matrix indicated at least partial mineralization. Embedded cells were numerous and the cells on the matrix surface were very large in size. Abundant rough endoplasmic reticulum and a prominent Golgi complex were evident. The presence of a split between the treated root surfaces and the newly formed tissue was a common observation, as was the presence of bacteria and host cells in the interfacial gap. Conclusion: Following treatment with EMD, a bone-like tissue resembling cellular intrinsic fibre cementum may develop on the root surfaces, instead of AEFC. Furthermore, EMD may both induce de novo formation of a mineralized connective tissue on scaled root surfaces and stimulate matrix deposition on old native cementum. Interfacial bonding appeared to be weak after 6 weeks of healing. Copyright © Blackwell Munksgaard Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/154302
ISSN
2013 Impact Factor: 2.215
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorBosshardt, DDen_US
dc.contributor.authorSculean, Aen_US
dc.contributor.authorWindisch, Pen_US
dc.contributor.authorPjetursson, BEen_US
dc.contributor.authorLang, NPen_US
dc.date.accessioned2012-08-08T08:24:30Z-
dc.date.available2012-08-08T08:24:30Z-
dc.date.issued2005en_US
dc.identifier.citationJournal Of Periodontal Research, 2005, v. 40 n. 2, p. 158-167en_US
dc.identifier.issn0022-3484en_US
dc.identifier.urihttp://hdl.handle.net/10722/154302-
dc.description.abstractObjective: Enamel matrix-derived proteins (EMD) are thought to trigger the formation of acellular extrinsic fibre cementum (AEFC), while other reports indicate that EMD may have osteogenic potential. The aim of the present study was to characterize the tissues developing on the root surface following application of EMD. Methods: Twelve human periodontitis-affected teeth, scheduled for extraction, were treated with EMD. Two to 6 weeks later, the teeth were extracted, demineralized and processed for embedding in acrylic and epoxy resins. New tissue formation was analysed by light and transmission electron microscopy. Results: New tissue formation on the root was observed in the notch and on both scaled and unscaled root surfaces distant of the notch area in six defects. The newly formed tissues on the root were thick, collagenous, devoid of extrinsic fibres, and had an irregular surface contour. The presence of electron-dense, organic material in the collagenous matrix indicated at least partial mineralization. Embedded cells were numerous and the cells on the matrix surface were very large in size. Abundant rough endoplasmic reticulum and a prominent Golgi complex were evident. The presence of a split between the treated root surfaces and the newly formed tissue was a common observation, as was the presence of bacteria and host cells in the interfacial gap. Conclusion: Following treatment with EMD, a bone-like tissue resembling cellular intrinsic fibre cementum may develop on the root surfaces, instead of AEFC. Furthermore, EMD may both induce de novo formation of a mineralized connective tissue on scaled root surfaces and stimulate matrix deposition on old native cementum. Interfacial bonding appeared to be weak after 6 weeks of healing. Copyright © Blackwell Munksgaard Ltd.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1en_US
dc.relation.ispartofJournal of Periodontal Researchen_US
dc.subject.meshCementogenesis - Physiologyen_US
dc.subject.meshDental Cementum - Drug Effectsen_US
dc.subject.meshDental Enamel Proteins - Therapeutic Useen_US
dc.subject.meshFemaleen_US
dc.subject.meshGuided Tissue Regeneration - Methodsen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPeriodontitis - Therapyen_US
dc.subject.meshTooth Root - Drug Effects - Physiology - Ultrastructureen_US
dc.titleEffects of enamel matrix proteins on tissue formation along the roots of human teethen_US
dc.typeArticleen_US
dc.identifier.emailLang, NP:nplang@hkucc.hku.hken_US
dc.identifier.authorityLang, NP=rp00031en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1600-0765.2005.00785.xen_US
dc.identifier.pmid15733151en_US
dc.identifier.scopuseid_2-s2.0-15744391739en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-15744391739&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume40en_US
dc.identifier.issue2en_US
dc.identifier.spage158en_US
dc.identifier.epage167en_US
dc.identifier.isiWOS:000227878600009-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridBosshardt, DD=6603806230en_US
dc.identifier.scopusauthoridSculean, A=7005695622en_US
dc.identifier.scopusauthoridWindisch, P=6701496175en_US
dc.identifier.scopusauthoridPjetursson, BE=6506841442en_US
dc.identifier.scopusauthoridLang, NP=7201577367en_US

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