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Article: Impact of sample storage on detection of periodontal bacteria

TitleImpact of sample storage on detection of periodontal bacteria
Authors
Issue Date2005
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/OMI
Citation
Oral Microbiology And Immunology, 2005, v. 20 n. 2, p. 128-130 How to Cite?
AbstractBackground/aims: Information on the impact of sample storage prior to analysis by DNA methods is limited. The aim of this study was to investigate the effect of subgingival sample storage on bacterial detection and enumeration. Material and methods: Subgingival plaque samples were studied by a) checkerboard DNA-DNA hybridization by immediate processing, b) storage at + 4°C for 6 weeks, c) storage at -20°C for 6 months or d) storage at -20°C for 12 months. Results: No differences in total DNA were found between protocol 1 and 2, or between protocol 3 and 4. Protocol 1 yielded 2.4 times more total bacterial DNA than did protocol 3 (P < 0.001). Actinobacillus actinomycetemcomitans and Campylobacter gracilis were detected in 21.1% of the immediately processed samples but only in 6.6% of the samples after 12 months of storage. Similar changes were noticed for Treponema denticola, which was detected in 22.3% and 9.2%, respectively. Streptococci spp., Fusobacterium nucleatum and Tannerella forsythia did not seem to be affected by storage. In contrast, the level of Campylobacter rectus detection frequency changed from 2.6% if processed immediately to 15.8% if samples were stored for 12 months. Conclusions: In longitudinal clinical studies including microbiological samples and processed with DNA-DNA hybridization methods, samples should be stored for the same period of time before processing to avoid loss of microbiological information. Copyright © Blackwell Munksgaard 2005.
Persistent Identifierhttp://hdl.handle.net/10722/154300
ISSN
2011 Impact Factor: 2.807
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKatsoulis, Jen_US
dc.contributor.authorHeitzMayfield, LJRen_US
dc.contributor.authorWeibel, Men_US
dc.contributor.authorHirschi, Ren_US
dc.contributor.authorLang, NPen_US
dc.contributor.authorPersson, GRen_US
dc.date.accessioned2012-08-08T08:24:29Z-
dc.date.available2012-08-08T08:24:29Z-
dc.date.issued2005en_US
dc.identifier.citationOral Microbiology And Immunology, 2005, v. 20 n. 2, p. 128-130en_US
dc.identifier.issn0902-0055en_US
dc.identifier.urihttp://hdl.handle.net/10722/154300-
dc.description.abstractBackground/aims: Information on the impact of sample storage prior to analysis by DNA methods is limited. The aim of this study was to investigate the effect of subgingival sample storage on bacterial detection and enumeration. Material and methods: Subgingival plaque samples were studied by a) checkerboard DNA-DNA hybridization by immediate processing, b) storage at + 4°C for 6 weeks, c) storage at -20°C for 6 months or d) storage at -20°C for 12 months. Results: No differences in total DNA were found between protocol 1 and 2, or between protocol 3 and 4. Protocol 1 yielded 2.4 times more total bacterial DNA than did protocol 3 (P < 0.001). Actinobacillus actinomycetemcomitans and Campylobacter gracilis were detected in 21.1% of the immediately processed samples but only in 6.6% of the samples after 12 months of storage. Similar changes were noticed for Treponema denticola, which was detected in 22.3% and 9.2%, respectively. Streptococci spp., Fusobacterium nucleatum and Tannerella forsythia did not seem to be affected by storage. In contrast, the level of Campylobacter rectus detection frequency changed from 2.6% if processed immediately to 15.8% if samples were stored for 12 months. Conclusions: In longitudinal clinical studies including microbiological samples and processed with DNA-DNA hybridization methods, samples should be stored for the same period of time before processing to avoid loss of microbiological information. Copyright © Blackwell Munksgaard 2005.en_US
dc.languageengen_US
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/OMIen_US
dc.relation.ispartofOral Microbiology and Immunologyen_US
dc.subject.meshAnalysis Of Varianceen_US
dc.subject.meshDna, Bacterial - Analysisen_US
dc.subject.meshDental Plaque - Microbiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshLinear Modelsen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshPeriodontitis - Microbiologyen_US
dc.subject.meshPilot Projectsen_US
dc.subject.meshPreservation, Biologicalen_US
dc.subject.meshStatistics, Nonparametricen_US
dc.titleImpact of sample storage on detection of periodontal bacteriaen_US
dc.typeArticleen_US
dc.identifier.emailLang, NP:nplang@hkucc.hku.hken_US
dc.identifier.authorityLang, NP=rp00031en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1399-302X.2004.00200.xen_US
dc.identifier.pmid15720575-
dc.identifier.scopuseid_2-s2.0-14944343243en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-14944343243&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume20en_US
dc.identifier.issue2en_US
dc.identifier.spage128en_US
dc.identifier.epage130en_US
dc.identifier.isiWOS:000227130300011-
dc.publisher.placeDenmarken_US
dc.identifier.scopusauthoridKatsoulis, J=8567429400en_US
dc.identifier.scopusauthoridHeitzMayfield, LJR=8234379800en_US
dc.identifier.scopusauthoridWeibel, M=36742623900en_US
dc.identifier.scopusauthoridHirschi, R=23988898400en_US
dc.identifier.scopusauthoridLang, NP=7201577367en_US
dc.identifier.scopusauthoridPersson, GR=7101853867en_US
dc.identifier.citeulike99528-

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