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Article: The expression of osteoprotegerin and the receptor activator of nuclear factor kappa B ligand in human periodontal ligament cells cultured with and without 1α,25-dihydroxyvitamin D3

TitleThe expression of osteoprotegerin and the receptor activator of nuclear factor kappa B ligand in human periodontal ligament cells cultured with and without 1α,25-dihydroxyvitamin D3
Authors
Issue Date2004
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
Citation
Archives Of Oral Biology, 2004, v. 49 n. 1, p. 71-76 How to Cite?
AbstractThe receptor activator of nuclear factor kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG), are important bone metabolism molecules, which directly control osteoclastogenesis. Periodontal ligament (PDL) cells play a vital role in maintaining the homeostasis of periodontal tissues, releasing cytokines to affect bone metabolism. The purpose of this study was to investigate the expression of OPG and RANKL in cultured human periodontal ligament cells (hPDLCs) derived from permanent teeth and the expression change after stimulation by 1α,25-dihydroxyvitamin D3 (1α,25(OH)2vitD3), a kind of bone resorption promoter. HPDLCs were cultured in the presence or absence of 10-8M 1α,25(OH)2vitD3 in vitro. The expression of mRNA for OPG and RANKL in hPDLCs during 6 days' culture was examined using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The level of secreted OPG protein in the culture medium during 6 days' culture was detected by enzyme-linked immunoabsorbent assay (ELISA). The result showed that OPG and RANKL were expressed by hPDLCs. OPG expression was down-regulated by 10-8M 1α,25(OH)2vitD3 in a time-dependent manner, while RANKL mRNA was up-regulated. The ratio of OPG/ RANKL was decreased. In conclusion, our findings suggest that hPDLCs may regulate the alveolar bone metabolism through the OPG/RANKL system. © 2003 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/154280
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.713
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Den_US
dc.contributor.authorYang, YQen_US
dc.contributor.authorLi, XTen_US
dc.contributor.authorFu, MKen_US
dc.date.accessioned2012-08-08T08:24:22Z-
dc.date.available2012-08-08T08:24:22Z-
dc.date.issued2004en_US
dc.identifier.citationArchives Of Oral Biology, 2004, v. 49 n. 1, p. 71-76en_US
dc.identifier.issn0003-9969en_US
dc.identifier.urihttp://hdl.handle.net/10722/154280-
dc.description.abstractThe receptor activator of nuclear factor kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG), are important bone metabolism molecules, which directly control osteoclastogenesis. Periodontal ligament (PDL) cells play a vital role in maintaining the homeostasis of periodontal tissues, releasing cytokines to affect bone metabolism. The purpose of this study was to investigate the expression of OPG and RANKL in cultured human periodontal ligament cells (hPDLCs) derived from permanent teeth and the expression change after stimulation by 1α,25-dihydroxyvitamin D3 (1α,25(OH)2vitD3), a kind of bone resorption promoter. HPDLCs were cultured in the presence or absence of 10-8M 1α,25(OH)2vitD3 in vitro. The expression of mRNA for OPG and RANKL in hPDLCs during 6 days' culture was examined using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The level of secreted OPG protein in the culture medium during 6 days' culture was detected by enzyme-linked immunoabsorbent assay (ELISA). The result showed that OPG and RANKL were expressed by hPDLCs. OPG expression was down-regulated by 10-8M 1α,25(OH)2vitD3 in a time-dependent manner, while RANKL mRNA was up-regulated. The ratio of OPG/ RANKL was decreased. In conclusion, our findings suggest that hPDLCs may regulate the alveolar bone metabolism through the OPG/RANKL system. © 2003 Elsevier Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbioen_US
dc.relation.ispartofArchives of Oral Biologyen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAnalysis Of Varianceen_US
dc.subject.meshBicuspiden_US
dc.subject.meshCalcitriol - Pharmacologyen_US
dc.subject.meshCarrier Proteins - Geneticsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshChilden_US
dc.subject.meshCulture Media - Chemistryen_US
dc.subject.meshGene Expression Regulation - Drug Effectsen_US
dc.subject.meshGlycoproteins - Analysis - Biosynthesis - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshMembrane Glycoproteins - Geneticsen_US
dc.subject.meshOsteoprotegerinen_US
dc.subject.meshPeriodontal Ligament - Chemistry - Drug Effects - Metabolismen_US
dc.subject.meshRank Liganden_US
dc.subject.meshReceptor Activator Of Nuclear Factor-Kappa Ben_US
dc.subject.meshReceptors, Cytoplasmic And Nuclear - Analysis - Biosynthesis - Geneticsen_US
dc.subject.meshReceptors, Tumor Necrosis Factoren_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.titleThe expression of osteoprotegerin and the receptor activator of nuclear factor kappa B ligand in human periodontal ligament cells cultured with and without 1α,25-dihydroxyvitamin D3en_US
dc.typeArticleen_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0003-9969(03)00201-2en_US
dc.identifier.pmid14693199-
dc.identifier.scopuseid_2-s2.0-0842289106en_US
dc.identifier.hkuros146504-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0842289106&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume49en_US
dc.identifier.issue1en_US
dc.identifier.spage71en_US
dc.identifier.epage76en_US
dc.identifier.isiWOS:000188418200009-
dc.publisher.placeUnited Kingdomen_US

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