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Article: Cbfa1 couples chondrocytes maturation and endochondral ossification in rat mandibular condylar cartilage

TitleCbfa1 couples chondrocytes maturation and endochondral ossification in rat mandibular condylar cartilage
Authors
KeywordsCbfa1
Condylar cartilage
Endochondral ossification
Issue Date2004
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
Citation
Archives of Oral Biology, 2004, v. 49 n. 2, p. 109-118 How to Cite?
AbstractCore binding factor a1 (Cbfa1) is a crucial transcription factor for osteoblasts differentiation and chondrocytes maturation in embryonic skeletal genesis, but little is known about its function in mandibular condylar growth. The aim of this study was to determine the temporal and spatial pattern of Cbfa1 expression in condylar cartilage during natural growth. Mandibular condyles were harvested from 50 female Sprague-Dawley rats at age of 38, 42, 49, 56 and 65 days. Alcian blue and PAS staining was used for histological analysis. Type A antibody raised against Cbfa1 isoform II was observed in the pre-hypertrophic and hypertrophic chondrocytes in condylar cartilage, and in the mature osteocytes in trabecular bone. Type B antibody raised against 17 aa sequence present after the Runt domain was detected in tartrate resistant acid phosphatase (TRAP) positive osteoclasts in the erosive front of cartilage, and also in the osteoblasts on the sub-chondral bone surface. In situ hybridisation was carried out with a probe containing a fragment in exon 8 of the cDNA. Cbfa1 transcripts were localised in the osteoblasts and chondrocytes, but not in osteoclasts. Quantitative analysis demonstrated that both types of Cbfa1 proteins reached their maximum level on day 56, which coincided with the terminal maturation of hypertrophic chondrocytes and the aggregation of mineralisation deposits in extracellular matrix. These results suggest that Cbfa1 is a master gene controlling the functions of all the skeletal cell lineages by synthesising different functional isoforms. Furthermore, Cbfa1 couples the process of chondrocytes maturation, extracellular matrix mineralisation and degradation, as well as osteoblasts invasion during endochondral bone formation. Beyond its function on embryonic development, Cbfa1 regulates the postnatal growth of mandibular condyle. © 2003 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/154279
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.713
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorRabie, ABMen_HK
dc.contributor.authorTang, GHen_HK
dc.contributor.authorHägg, Uen_HK
dc.date.accessioned2012-08-08T08:24:22Z-
dc.date.available2012-08-08T08:24:22Z-
dc.date.issued2004en_HK
dc.identifier.citationArchives of Oral Biology, 2004, v. 49 n. 2, p. 109-118en_HK
dc.identifier.issn0003-9969en_HK
dc.identifier.urihttp://hdl.handle.net/10722/154279-
dc.description.abstractCore binding factor a1 (Cbfa1) is a crucial transcription factor for osteoblasts differentiation and chondrocytes maturation in embryonic skeletal genesis, but little is known about its function in mandibular condylar growth. The aim of this study was to determine the temporal and spatial pattern of Cbfa1 expression in condylar cartilage during natural growth. Mandibular condyles were harvested from 50 female Sprague-Dawley rats at age of 38, 42, 49, 56 and 65 days. Alcian blue and PAS staining was used for histological analysis. Type A antibody raised against Cbfa1 isoform II was observed in the pre-hypertrophic and hypertrophic chondrocytes in condylar cartilage, and in the mature osteocytes in trabecular bone. Type B antibody raised against 17 aa sequence present after the Runt domain was detected in tartrate resistant acid phosphatase (TRAP) positive osteoclasts in the erosive front of cartilage, and also in the osteoblasts on the sub-chondral bone surface. In situ hybridisation was carried out with a probe containing a fragment in exon 8 of the cDNA. Cbfa1 transcripts were localised in the osteoblasts and chondrocytes, but not in osteoclasts. Quantitative analysis demonstrated that both types of Cbfa1 proteins reached their maximum level on day 56, which coincided with the terminal maturation of hypertrophic chondrocytes and the aggregation of mineralisation deposits in extracellular matrix. These results suggest that Cbfa1 is a master gene controlling the functions of all the skeletal cell lineages by synthesising different functional isoforms. Furthermore, Cbfa1 couples the process of chondrocytes maturation, extracellular matrix mineralisation and degradation, as well as osteoblasts invasion during endochondral bone formation. Beyond its function on embryonic development, Cbfa1 regulates the postnatal growth of mandibular condyle. © 2003 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbioen_HK
dc.relation.ispartofArchives of Oral Biologyen_HK
dc.subjectCbfa1en_HK
dc.subjectCondylar cartilageen_HK
dc.subjectEndochondral ossificationen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshCalcification, Physiologic - Genetics - Physiologyen_US
dc.subject.meshCartilage - Growth & Development - Metabolismen_US
dc.subject.meshChondrocytes - Physiologyen_US
dc.subject.meshCore Binding Factor Alpha 1 Subuniten_US
dc.subject.meshCore Binding Factorsen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression - Geneticsen_US
dc.subject.meshMandibular Condyle - Growth & Development - Metabolismen_US
dc.subject.meshNeoplasm Proteins - Analysis - Physiologyen_US
dc.subject.meshOsteogenesis - Genetics - Physiologyen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshTibia - Metabolismen_US
dc.subject.meshTranscription Factors - Analysis - Physiologyen_US
dc.titleCbfa1 couples chondrocytes maturation and endochondral ossification in rat mandibular condylar cartilageen_HK
dc.typeArticleen_HK
dc.identifier.emailRabie, ABM: rabie@hku.hken_HK
dc.identifier.emailHägg, U: euohagg@hkusua.hku.hken_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.identifier.authorityHägg, U=rp00020en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.archoralbio.2003.09.006en_HK
dc.identifier.pmid14693204-
dc.identifier.scopuseid_2-s2.0-0742323606en_HK
dc.identifier.hkuros95053-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0742323606&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume49en_HK
dc.identifier.issue2en_HK
dc.identifier.spage109en_HK
dc.identifier.epage118en_HK
dc.identifier.isiWOS:000189228200003-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridRabie, ABM=7007172734en_HK
dc.identifier.scopusauthoridTang, GH=9842004300en_HK
dc.identifier.scopusauthoridHägg, U=7006790279en_HK

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