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Article: Direct detection of Actinomyces spp. from infected root canals in a Chinese population: A study using PCR-based, oligonucleotide-DNA hybridization technique

TitleDirect detection of Actinomyces spp. from infected root canals in a Chinese population: A study using PCR-based, oligonucleotide-DNA hybridization technique
Authors
KeywordsActinomycetes
Chinese
Oligonucleotide-DNA hybridization
Polymerase chain reaction
Root canal infection
Issue Date2003
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent
Citation
Journal Of Dentistry, 2003, v. 31 n. 8, p. 559-568 How to Cite?
AbstractObjectives. The poor sensitivity of phenotypic identification techniques has hampered the taxonomic differentiation of Actinomyces. Hence we developed a sensitive and specific, PCR-based oligonucleotide-DNA hybridization technique to detect Actinomyces spp. and, used this method to detect these organisms in samples directly obtained from infected root canals. Methods. A total of 32 samples from 28 Chinese patients, with primary root canal infections, aseptically exposed at the first patient visit, were studied. Whole bacterial genomic DNA was isolated directly from paper point samples. The variable regions of 16S ribosomal DNA of bacteria were amplified and labeled with digoxigenin for further hybridization and detection. A total of seven oligonucleotide probes specific for A. bovis, A. gerencseriae, A. israelii, A. meyeri, catalase-negative A. naeslundii (genospecies 1 and 2), catalase-positive A. naeslundii genospecies 2 and A. odontolyticus were used. Results. 16 of the 32 teeth were infected with one or more Actinomyces species. The prevalence rates of the examined species were: A. odontolyticus 31.3%, A. meyeri 9.4%, A. naeslundii 9.4%, A. israelii 6.3% and A. gerencseriae 3.1%; no A. bovis was detected in any of the canals. Furthermore, A. odontolyticus was isolated more frequently from root canals with caries or a history of caries (Fisher's exact test: P=0.0496; Odds ratio=9.00, 95% confidence interval: 0.97-83.63), and A. naeslundii was significantly associated with traumatized teeth (Fisher's exact test: P=0.0121; Odds ratio=57.00, 95% confidence interval: 2.10-1546.90). However, no significant correlation was found between Actinomyces spp. and clinical symptoms and signs, such as pain, swelling, percussion to tenderness, sinus and periapical radiolucency. Conclusion. Actinomyces spp. may be important pathogens of root canal infections. A. naeslundii in particular may be related with traumatized teeth. A. odontolyticus appears to be involved in infections related to caries, exposure of dentinal tubules during cavity preparation and/or leaking restoration, but further clarification with large samples is necessary. © 2003 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/154252
ISSN
2015 Impact Factor: 3.109
2015 SCImago Journal Rankings: 1.029
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTang, Gen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorYip, HKen_HK
dc.contributor.authorChu, FCSen_HK
dc.contributor.authorTsang, PCSen_HK
dc.contributor.authorCheung, BPKen_HK
dc.date.accessioned2012-08-08T08:24:12Z-
dc.date.available2012-08-08T08:24:12Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal Of Dentistry, 2003, v. 31 n. 8, p. 559-568en_HK
dc.identifier.issn0300-5712en_HK
dc.identifier.urihttp://hdl.handle.net/10722/154252-
dc.description.abstractObjectives. The poor sensitivity of phenotypic identification techniques has hampered the taxonomic differentiation of Actinomyces. Hence we developed a sensitive and specific, PCR-based oligonucleotide-DNA hybridization technique to detect Actinomyces spp. and, used this method to detect these organisms in samples directly obtained from infected root canals. Methods. A total of 32 samples from 28 Chinese patients, with primary root canal infections, aseptically exposed at the first patient visit, were studied. Whole bacterial genomic DNA was isolated directly from paper point samples. The variable regions of 16S ribosomal DNA of bacteria were amplified and labeled with digoxigenin for further hybridization and detection. A total of seven oligonucleotide probes specific for A. bovis, A. gerencseriae, A. israelii, A. meyeri, catalase-negative A. naeslundii (genospecies 1 and 2), catalase-positive A. naeslundii genospecies 2 and A. odontolyticus were used. Results. 16 of the 32 teeth were infected with one or more Actinomyces species. The prevalence rates of the examined species were: A. odontolyticus 31.3%, A. meyeri 9.4%, A. naeslundii 9.4%, A. israelii 6.3% and A. gerencseriae 3.1%; no A. bovis was detected in any of the canals. Furthermore, A. odontolyticus was isolated more frequently from root canals with caries or a history of caries (Fisher's exact test: P=0.0496; Odds ratio=9.00, 95% confidence interval: 0.97-83.63), and A. naeslundii was significantly associated with traumatized teeth (Fisher's exact test: P=0.0121; Odds ratio=57.00, 95% confidence interval: 2.10-1546.90). However, no significant correlation was found between Actinomyces spp. and clinical symptoms and signs, such as pain, swelling, percussion to tenderness, sinus and periapical radiolucency. Conclusion. Actinomyces spp. may be important pathogens of root canal infections. A. naeslundii in particular may be related with traumatized teeth. A. odontolyticus appears to be involved in infections related to caries, exposure of dentinal tubules during cavity preparation and/or leaking restoration, but further clarification with large samples is necessary. © 2003 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdenten_HK
dc.relation.ispartofJournal of Dentistryen_HK
dc.subjectActinomycetesen_HK
dc.subjectChineseen_HK
dc.subjectOligonucleotide-DNA hybridizationen_HK
dc.subjectPolymerase chain reactionen_HK
dc.subjectRoot canal infectionen_HK
dc.subject.meshActinomyces - Classification - Isolation & Purificationen_US
dc.subject.meshActinomycosis - Microbiologyen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshChinaen_US
dc.subject.meshConfidence Intervalsen_US
dc.subject.meshDna, Bacterial - Analysisen_US
dc.subject.meshDental Caries - Microbiologyen_US
dc.subject.meshDental Pulp Cavity - Microbiologyen_US
dc.subject.meshDental Pulp Diseases - Microbiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGenotypeen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshOdds Ratioen_US
dc.subject.meshOligonucleotide Probesen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshRna, Ribosomal, 16S - Analysisen_US
dc.subject.meshTooth Injuries - Microbiologyen_US
dc.titleDirect detection of Actinomyces spp. from infected root canals in a Chinese population: A study using PCR-based, oligonucleotide-DNA hybridization techniqueen_HK
dc.typeArticleen_HK
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_HK
dc.identifier.emailYip, HK: kevin.h.k.yip@hkusua.hku.hken_HK
dc.identifier.emailChu, FCS: cschu@hkucc.hku.hken_HK
dc.identifier.emailTsang, PCS: csptsang@hkucc.hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.identifier.authorityYip, HK=rp00027en_HK
dc.identifier.authorityChu, FCS=rp00035en_HK
dc.identifier.authorityTsang, PCS=rp00026en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0300-5712(03)00112-Xen_HK
dc.identifier.pmid14554073-
dc.identifier.scopuseid_2-s2.0-0141838584en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0141838584&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume31en_HK
dc.identifier.issue8en_HK
dc.identifier.spage559en_HK
dc.identifier.epage568en_HK
dc.identifier.isiWOS:000186001500006-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridTang, G=36882453100en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridYip, HK=25423244900en_HK
dc.identifier.scopusauthoridChu, FCS=7201881096en_HK
dc.identifier.scopusauthoridTsang, PCS=7202936002en_HK
dc.identifier.scopusauthoridCheung, BPK=7103294773en_HK

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